PMID- 26525667 OWN - NLM STAT- MEDLINE DCOM- 20161020 LR - 20170103 IS - 1873-4847 (Electronic) IS - 0955-2863 (Linking) VI - 27 DP - 2016 Jan TI - Dietary extra virgin olive oil attenuates kidney injury in pristane-induced SLE model via activation of HO-1/Nrf-2 antioxidant pathway and suppression of JAK/STAT, NF-kappaB and MAPK activation. PG - 278-88 LID - S0955-2863(15)00253-3 [pii] LID - 10.1016/j.jnutbio.2015.09.017 [doi] AB - Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by a widespread organ involvement. Recent studies have suggested that extra virgin olive oil (EVOO) might possess preventive effects on this immunoinflammation-related disease. However, its role in SLE remained unknown. In this work, we evaluated the effects of EVOO diet in a pristane-induced SLE model in mice. Three-month-old mice received an injection of pristane or saline solution and were fed with different experimental diets: sunflower oil diet or EVOO diet. After 24weeks, mice were sacrificed, spleens were collected and kidneys were removed for immunoinflammatory detections. The kidney expression of microsomal prostaglandin E synthase 1, heme oxygenase 1 (HO-1), nuclear factor E2-related factor 2 (Nrf-2), mitogen-activated protein kinases (MAPKs), Janus kinase/signal transducer and activator of transcription (JAK/STAT) and nuclear transcription factor-kappa B (NF-kappaB) pathways were studied by western blotting. In addition to macroscopic and histological analyses, serum matrix metalloproteinase 3 (MMP-3) levels and proinflammatory cytokines production in splenocytes were evaluated by enzyme-linked immunoassay. We have demonstrated that EVOO diet significantly reduced renal damage and decreased MMP-3 serum and PGE2 kidney levels as well as the proinflammatory cytokines production in splenocytes. Our data indicate that Nrf-2 and HO-1 protein expressions were up-regulated in those mice fed with EVOO and the activation of JAK/STAT, MAPK and NF-kappaB pathways were drastically ameliorated. These results support the interest of EVOO as a beneficial functional food exerting a preventive/palliative role in the management of SLE. CI - Copyright (c) 2015 Elsevier Inc. All rights reserved. FAU - Aparicio-Soto, Marina AU - Aparicio-Soto M AD - Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41004 Sevilla, Spain. FAU - Sanchez-Hidalgo, Marina AU - Sanchez-Hidalgo M AD - Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41004 Sevilla, Spain. FAU - Cardeno, Ana AU - Cardeno A AD - Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41004 Sevilla, Spain. FAU - Rosillo, Maria Angeles AU - Rosillo MA AD - Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41004 Sevilla, Spain. FAU - Sanchez-Fidalgo, Susana AU - Sanchez-Fidalgo S AD - Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41004 Sevilla, Spain. FAU - Utrilla, Jose AU - Utrilla J AD - Department of Cytology and Histology, School of Medicine, University of Seville, 41004 Sevilla, Spain. FAU - Martin-Lacave, Ines AU - Martin-Lacave I AD - Department of Cytology and Histology, School of Medicine, University of Seville, 41004 Sevilla, Spain. FAU - Alarcon-de-la-Lastra, Catalina AU - Alarcon-de-la-Lastra C AD - Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41004 Sevilla, Spain. Electronic address: calarcon@us.es. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20151029 PL - United States TA - J Nutr Biochem JT - The Journal of nutritional biochemistry JID - 9010081 RN - 0 (Antioxidants) RN - 0 (Cytokines) RN - 0 (NF-E2-Related Factor 2) RN - 0 (NF-kappa B) RN - 0 (Nfe2l2 protein, mouse) RN - 0 (Olive Oil) RN - EC 1.14.14.18 (Heme Oxygenase-1) RN - EC 5.3.- (Intramolecular Oxidoreductases) RN - EC 5.3.99.3 (Prostaglandin-E Synthases) SB - IM MH - Animals MH - Antioxidants/*metabolism MH - Cytokines/biosynthesis MH - *Disease Models, Animal MH - Down-Regulation MH - Female MH - Heme Oxygenase-1/*metabolism MH - Intramolecular Oxidoreductases/metabolism MH - Kidney Diseases/*etiology MH - Lupus Erythematosus, Systemic/*complications MH - *MAP Kinase Signaling System MH - Mice MH - Mice, Inbred BALB C MH - NF-E2-Related Factor 2/*metabolism MH - NF-kappa B/metabolism MH - Olive Oil/*administration & dosage MH - Prostaglandin-E Synthases OTO - NOTNLM OT - EVOO OT - Immunomodulation OT - Inflammation OT - Polyphenols OT - SLE EDAT- 2015/11/04 06:00 MHDA- 2016/10/21 06:00 CRDT- 2015/11/04 06:00 PHST- 2015/03/20 00:00 [received] PHST- 2015/09/14 00:00 [revised] PHST- 2015/09/18 00:00 [accepted] PHST- 2015/11/04 06:00 [entrez] PHST- 2015/11/04 06:00 [pubmed] PHST- 2016/10/21 06:00 [medline] AID - S0955-2863(15)00253-3 [pii] AID - 10.1016/j.jnutbio.2015.09.017 [doi] PST - ppublish SO - J Nutr Biochem. 2016 Jan;27:278-88. doi: 10.1016/j.jnutbio.2015.09.017. Epub 2015 Oct 29.