PMID- 26526910
OWN - NLM
STAT- MEDLINE
DCOM- 20160708
LR  - 20151103
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 898
DP  - 2015 Oct 22
TI  - Porous membrane-protected molecularly imprinted polymer micro-solid-phase 
      extraction for analysis of urinary cocaine and its metabolites using liquid 
      chromatography - Tandem mass spectrometry.
PG  - 50-9
LID - S0003-2670(15)01236-2 [pii]
LID - 10.1016/j.aca.2015.10.002 [doi]
AB  - Porous membrane-protected micro-solid phase extraction (mu-SPE) using a 
      molecularly imprinted polymer (MIP) as an adsorbent has been proposed as an 
      integrated extraction-cleanup procedure for isolating cocaine (COC) and its 
      metabolites [benzoylecgonine (BZE), ecgonine methyl ester (EME), and cocaethylene 
      (CE)] from human urine. MIP beads have been synthesized using COC as a template 
      molecule, ethylene dimethacrylate (EDMA) as a functional monomer, divinylbenzene 
      (DVB) as a cross-linker, and 2,2'-azobisisobutyronitrile (AIBN) as an initiator. 
      High performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS) 
      has been used for quantifying the analytes after MIP-mu-SPE. Variables such as 
      urine pH, adsorption temperature and time, mechanical (orbital-horizontal) 
      stirring; and composition of the eluting solution and eluting time, were 
      evaluated. The proposed method was shown to be precise and accurate [relative 
      standard deviations (RSDs) of intra- and inter-day tests ranging from 3 to 8% and 
      from 2 to 10%, respectively]; and analytical recoveries in the range of 89-100%). 
      In addition, excellent accuracy was also verified after analyzing a FDT +25% 
      control material for BZE. The detection limits were in the range of 
      0.16-1.7 ng L(-1), low enough for confirmative conclusions regarding cocaine 
      abuse. The method was finally applied for screening/quantifying cocaine and 
      metabolites in urine samples from poly-drug abusers.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Sanchez-Gonzalez, Juan
AU  - Sanchez-Gonzalez J
AD  - Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of 
      Chemistry, University of Santiago de Compostela, Avenida das Ciencias, s/n, 
      15782, Santiago de Compostela, Spain.
FAU - Tabernero, Maria Jesus
AU  - Tabernero MJ
AD  - Department of Pathologic Anatomy and Forensic Sciences, Faculty of Medicine, 
      University of Santiago de Compostela, Rua de San Francisco, s/n, 15782, Santiago 
      de Compostela, Spain.
FAU - Bermejo, Ana Maria
AU  - Bermejo AM
AD  - Department of Pathologic Anatomy and Forensic Sciences, Faculty of Medicine, 
      University of Santiago de Compostela, Rua de San Francisco, s/n, 15782, Santiago 
      de Compostela, Spain.
FAU - Bermejo-Barrera, Pilar
AU  - Bermejo-Barrera P
AD  - Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of 
      Chemistry, University of Santiago de Compostela, Avenida das Ciencias, s/n, 
      15782, Santiago de Compostela, Spain.
FAU - Moreda-Pineiro, Antonio
AU  - Moreda-Pineiro A
AD  - Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of 
      Chemistry, University of Santiago de Compostela, Avenida das Ciencias, s/n, 
      15782, Santiago de Compostela, Spain. Electronic address: antonio.moreda@usc.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151021
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Polymers)
RN  - I5Y540LHVR (Cocaine)
SB  - IM
MH  - Chromatography, Liquid/*methods
MH  - Cocaine/*urine
MH  - Humans
MH  - Microscopy, Electron, Scanning
MH  - *Molecular Imprinting
MH  - Polymers/*chemistry
MH  - Porosity
MH  - Reproducibility of Results
MH  - Solid Phase Microextraction/*methods
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Cocaine
OT  - High performance liquid chromatography tandem mass spectrometry
OT  - Human urine
OT  - Micro-solid phase extraction
OT  - Molecularly imprinted polymer
OT  - Porous membrane-protected
EDAT- 2015/11/04 06:00
MHDA- 2016/07/09 06:00
CRDT- 2015/11/04 06:00
PHST- 2015/07/31 00:00 [received]
PHST- 2015/09/30 00:00 [revised]
PHST- 2015/10/07 00:00 [accepted]
PHST- 2015/11/04 06:00 [entrez]
PHST- 2015/11/04 06:00 [pubmed]
PHST- 2016/07/09 06:00 [medline]
AID - S0003-2670(15)01236-2 [pii]
AID - 10.1016/j.aca.2015.10.002 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2015 Oct 22;898:50-9. doi: 10.1016/j.aca.2015.10.002. Epub 2015 
      Oct 21.