PMID- 26526911
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160202
LR  - 20151103
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 898
DP  - 2015 Oct 22
TI  - Analytical capabilities of high performance liquid chromatography - Atmospheric 
      pressure photoionization - Orbitrap mass spectrometry (HPLC-APPI-Orbitrap-MS) for 
      the trace determination of novel and emerging flame retardants in fish.
PG  - 60-72
LID - S0003-2670(15)01242-8 [pii]
LID - 10.1016/j.aca.2015.10.008 [doi]
AB  - A new analytical method was established and validated for the analysis of 27 
      brominated flame retardants (BFRs), including so called "emerging" and "novel" 
      BFRs (EBFRs and NBFRs) in fish samples. High performance liquid chromatography 
      (HPLC) coupled to Orbitrap mass spectrometry (Orbitrap-MS) employing atmospheric 
      pressure photoionization (APPI) interface operated in negative mode was used for 
      the identification/quantitation of contaminants. HPLC-Orbitrap-MS analysis 
      provided a fast separation of selected analytes within 14 min, thus demonstrating 
      a high throughput processing of samples. The developed methodology was tested by 
      intralaboratory validation in terms of recovery, repeatability, linear 
      calibration ranges, instrumental and method limits of quantitation (i-LOQ and 
      m-LOQ), and where possible, trueness was verified by analysis of certified 
      reference materials (CRMs). Recoveries of analytes were between 80 and 119%, 
      while the repeatability in terms of relative standard deviations (RSDs) was in 
      the range from 1.2 to 15.5%. The measured values for both analyzed CRMs agreed 
      with the provided consensus values, revealing the recovery of reference 
      concentrations in 72-119% range. The elaborated method met the sensitivity 
      criterion according to Commission Recommendation 2014/118/EU on monitoring of 
      BFRs in food products for majority of the compounds. The concentrations of 
      polybrominated diphenyl ethers (PBDEs) in real samples determined by 
      HPLC-APPI-Orbitrap-MS method and validated gas chromatography-high-resolution 
      mass spectrometry (GC-HRMS) method were found to be in a good agreement.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Zacs, D
AU  - Zacs D
AD  - Institute of Food Safety, Animal Health and Environment "BIOR", Lejupes iela 3, 
      Riga, LV-1076, Latvia. Electronic address: dzintars.zachs@gmail.com.
FAU - Bartkevics, V
AU  - Bartkevics V
AD  - Institute of Food Safety, Animal Health and Environment "BIOR", Lejupes iela 3, 
      Riga, LV-1076, Latvia; University of Latvia, Department of Chemistry, Kr. 
      Valdemara iela 48, Riga, LV-1013, Latvia.
LA  - eng
PT  - Journal Article
DEP - 20151020
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
OTO - NOTNLM
OT  - Atmospheric pressure photoionization
OT  - Brominated flame retardants
OT  - Liquid chromatography
OT  - Orbitrap mass spectrometry
EDAT- 2015/11/04 06:00
MHDA- 2015/11/04 06:01
CRDT- 2015/11/04 06:00
PHST- 2015/06/22 00:00 [received]
PHST- 2015/09/22 00:00 [revised]
PHST- 2015/10/05 00:00 [accepted]
PHST- 2015/11/04 06:00 [entrez]
PHST- 2015/11/04 06:00 [pubmed]
PHST- 2015/11/04 06:01 [medline]
AID - S0003-2670(15)01242-8 [pii]
AID - 10.1016/j.aca.2015.10.008 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2015 Oct 22;898:60-72. doi: 10.1016/j.aca.2015.10.008. Epub 2015 
      Oct 20.