PMID- 26526992
OWN - NLM
STAT- MEDLINE
DCOM- 20160824
LR  - 20181202
IS  - 2211-1247 (Electronic)
VI  - 13
IP  - 6
DP  - 2015 Nov 10
TI  - MicroRNA-223 Regulates the Differentiation and Function of Intestinal Dendritic 
      Cells and Macrophages by Targeting C/EBPbeta.
PG  - 1149-1160
LID - S2211-1247(15)01115-8 [pii]
LID - 10.1016/j.celrep.2015.09.073 [doi]
AB  - Dendritic cells (DCs) and macrophages play important roles in maintaining 
      intestinal homeostasis. However, the molecular mechanisms that regulate the 
      differentiation and responses of intestinal DCs and macrophages remain poorly 
      understood. Here, we have identified microRNA miR-223 as a key molecule for 
      regulating these processes. Deficiency of miR-223 led to a significantly 
      decreased number of intestinal CX3CR1(hi) macrophages at steady state. Both 
      intestinal CX3CR1(hi) macrophages and CD103(+) conventional DCs (cDCs) in 
      miR-223-deficient mice exhibited a strong pro-inflammatory phenotype. Moreover, 
      miR-223-deficient monocytes gave rise to more monocyte-derived DCs (moDCs) and 
      produced more pro-inflammatory cytokines upon stimulation. Using a mouse model of 
      colitis, we demonstrated that the miR-223 deficiency resulted in more severe 
      colitis. Target gene analysis further identified that the effects of miR-223 on 
      DCs and macrophages were mediated by directly targeting C/EBPbeta. Taken together, 
      our study identifies a role for miR-223 as a critical regulator of intestinal 
      homeostasis.
CI  - Copyright (c) 2015 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Zhou, Haibo
AU  - Zhou H
AD  - Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University School of 
      Medicine, Beijing 100084, China.
FAU - Xiao, Jing
AU  - Xiao J
AD  - Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University School of 
      Medicine, Beijing 100084, China.
FAU - Wu, Ning
AU  - Wu N
AD  - Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University School of 
      Medicine, Beijing 100084, China.
FAU - Liu, Chunxi
AU  - Liu C
AD  - Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University School of 
      Medicine, Beijing 100084, China.
FAU - Xu, Jie
AU  - Xu J
AD  - Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University School of 
      Medicine, Beijing 100084, China.
FAU - Liu, Fang
AU  - Liu F
AD  - Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University School of 
      Medicine, Beijing 100084, China.
FAU - Wu, Li
AU  - Wu L
AD  - Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University School of 
      Medicine, Beijing 100084, China. Electronic address: wuli@tsinghua.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Rep
JT  - Cell reports
JID - 101573691
RN  - 0 (CCAAT-Enhancer-Binding Protein-beta)
RN  - 0 (CX3C Chemokine Receptor 1)
RN  - 0 (Cebpb protein, mouse)
RN  - 0 (Cx3cr1 protein, mouse)
RN  - 0 (MIRN223 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Animals
MH  - CCAAT-Enhancer-Binding Protein-beta/*genetics/metabolism
MH  - CX3C Chemokine Receptor 1
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Colitis/*metabolism
MH  - Dendritic Cells/cytology/*metabolism
MH  - Intestinal Mucosa/metabolism
MH  - Intestines/cytology
MH  - Macrophages/cytology/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*genetics
MH  - Receptors, Chemokine/genetics/metabolism
EDAT- 2015/11/04 06:00
MHDA- 2016/08/25 06:00
CRDT- 2015/11/04 06:00
PHST- 2015/02/12 00:00 [received]
PHST- 2015/07/31 00:00 [revised]
PHST- 2015/09/24 00:00 [accepted]
PHST- 2015/11/04 06:00 [entrez]
PHST- 2015/11/04 06:00 [pubmed]
PHST- 2016/08/25 06:00 [medline]
AID - S2211-1247(15)01115-8 [pii]
AID - 10.1016/j.celrep.2015.09.073 [doi]
PST - ppublish
SO  - Cell Rep. 2015 Nov 10;13(6):1149-1160. doi: 10.1016/j.celrep.2015.09.073.