PMID- 26527255
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20190827
IS  - 1096-0805 (Electronic)
IS  - 0022-2011 (Linking)
VI  - 132
DP  - 2015 Nov
TI  - Detection of undescribed ostreid herpesvirus 1 (OsHV-1) specimens from Pacific 
      oyster, Crassostrea gigas.
PG  - 182-189
LID - S0022-2011(15)30025-2 [pii]
LID - 10.1016/j.jip.2015.10.005 [doi]
AB  - The ostreid herpesvirus 1 (OsHV-1) and variants were implicated in mass mortality 
      affecting the young Pacific cupped oysters, Crassostrea gigas, in European 
      countries and those around the world. From 2008 onwards, oyster mortality had 
      greatly increased on the French coast and was associated with the detection of a 
      new OsHV-1 variant, entitled OsHV-1 muVar. The OsHV-1 muVar is predominant in 
      oysters; however, other OsHV-1 variants have been detected in samples collected 
      during mortality periods or collected out of mortality periods in France, 
      Ireland, Spain, Portugal, Italy, Mexico, United States, South Korea, Australia, 
      and New Zealand. A retrospective study conducted on 1047 OsHV-1 specimens sampled 
      mainly in France between 2009 and 2012, revealed 17 undescribed OsHV-1 variants 
      found in 65 oyster samples. These specimens presented point mutations situated 
      downstream and upstream from the microsatellite area in the C region (ORF 4/5) 
      which were different from the OsHV-1 reference and the OsHV-1 muVar. In the 
      present work, investigation was performed to further characterize these OsHV-1 
      specimens by sequencing two habitually targeted regions to study genetic 
      polymorphism of the virus: ORF 41/42 and ORF 35-38. An OsHV-1 variant detected in 
      six oyster samples, contained a nucleotide substitution in the C region which 
      impacted the amino acid sequence and might modify the function of the unknown 
      protein encoding by ORF 4. For the ORF 41/42 region, only two specimens presented 
      a synonymous mutation in comparison with the OsHV-1 muVar. All specimens contained 
      the same deletion with the OsHV-1 muVar in ORF 35-38. Then, a phylogenetic 
      analysis based on the C region was performed to investigate the distribution of 
      undescribed specimens among 21 OsHV-1 DNA sequences notified in GenBank and 
      collected from different countries (France, Japan, New Zealand, China, Ireland, 
      and United States) between 1995 and 2012. All analyzed samples and the OsHV-1 
      muVar were placed in the same group, excepted for a Japan specimen. Our results 
      contribute to improve the description of the genetic diversity of the OsHV-1 and 
      the C region (ORF 4/5) appears to be a better target than ORF 42/42 and 35-38 to 
      distinguish variants between themselves.
CI  - Crown Copyright (c) 2015. Published by Elsevier Inc. All rights reserved.
FAU - Martenot, Claire
AU  - Martenot C
AD  - LABEO Frank Duncombe, 1 Route de Rosel, 14053 Caen Cedex 4, France; ICORE, 
      Universite de Caen Basse-Normandie, Caen Cedex 14032, France; CNRS INEE, FRE 3484 
      BOREA, Caen Cedex 14032, France. Electronic address: claire.martenot@calvados.fr.
FAU - Lethuillier, Ophelie
AU  - Lethuillier O
AD  - LABEO Frank Duncombe, 1 Route de Rosel, 14053 Caen Cedex 4, France.
FAU - Fourour, Sarah
AU  - Fourour S
AD  - LABEO Frank Duncombe, 1 Route de Rosel, 14053 Caen Cedex 4, France.
FAU - Oden, Elise
AU  - Oden E
AD  - LABEO Frank Duncombe, 1 Route de Rosel, 14053 Caen Cedex 4, France; ICORE, 
      Universite de Caen Basse-Normandie, Caen Cedex 14032, France; CNRS INEE, FRE 3484 
      BOREA, Caen Cedex 14032, France.
FAU - Trancart, Suzanne
AU  - Trancart S
AD  - LABEO Frank Duncombe, 1 Route de Rosel, 14053 Caen Cedex 4, France.
FAU - Travaille, Emmanuelle
AU  - Travaille E
AD  - LABEO Frank Duncombe, 1 Route de Rosel, 14053 Caen Cedex 4, France; ICORE, 
      Universite de Caen Basse-Normandie, Caen Cedex 14032, France; CNRS INEE, FRE 3484 
      BOREA, Caen Cedex 14032, France.
FAU - Houssin, Maryline
AU  - Houssin M
AD  - LABEO Frank Duncombe, 1 Route de Rosel, 14053 Caen Cedex 4, France; ICORE, 
      Universite de Caen Basse-Normandie, Caen Cedex 14032, France; CNRS INEE, FRE 3484 
      BOREA, Caen Cedex 14032, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151023
PL  - United States
TA  - J Invertebr Pathol
JT  - Journal of invertebrate pathology
JID - 0014067
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Crassostrea/*virology
MH  - DNA, Viral/chemistry
MH  - Ecthyma, Contagious
MH  - France
MH  - Genetic Variation
MH  - Herpesviridae/*genetics/isolation & purification
MH  - Microsatellite Repeats
MH  - Phylogeny
MH  - Point Mutation
MH  - Retrospective Studies
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - Crassostrea gigas
OT  - Genetic diversity
OT  - OsHV-1
OT  - Variants
EDAT- 2015/11/04 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/11/04 06:00
PHST- 2015/08/24 00:00 [received]
PHST- 2015/10/16 00:00 [revised]
PHST- 2015/10/20 00:00 [accepted]
PHST- 2015/11/04 06:00 [entrez]
PHST- 2015/11/04 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S0022-2011(15)30025-2 [pii]
AID - 10.1016/j.jip.2015.10.005 [doi]
PST - ppublish
SO  - J Invertebr Pathol. 2015 Nov;132:182-189. doi: 10.1016/j.jip.2015.10.005. Epub 
      2015 Oct 23.