PMID- 26528374
OWN - NLM
STAT- Publisher
LR  - 20191120
IS  - 1759-9660 (Print)
IS  - 1759-9679 (Electronic)
IS  - 1759-9660 (Linking)
VI  - 7
IP  - 20
DP  - 2015 Oct 21
TI  - Magnetically-actuated, bead-enhanced silicon photonic immunosensor.
PG  - 8539-8544
AB  - Magnetic actuation has been introduced to an optical immunosensor technology 
      resulting in improvements in both rapidity and limit of detection for an assay 
      quantitating low concentrations of a representative protein biomarker. For 
      purposes of demonstration, an assay was designed for monocyte chemotactic protein 
      1 (MCP-1), a small cytokine which regulates migration and infiltration of 
      monocytes and macrophages, and is an emerging biomarker for several diseases. The 
      immunosensor is based on arrays of highly multiplexed silicon photonic microring 
      resonators. A one-step sandwich immunoassay was performed and the signal was 
      further enhanced through a tertiary recognition event between biotinylated tracer 
      antibodies and streptavidin-coated magnetic beads. By integrating a magnet under 
      the sensor chip, magnetic beads were rapidly directed towards the sensor surface 
      resulting in improved assay performance metrics. Notably, the time required in 
      the bead binding step was reduced by a factor of 11 (4 vs 45 min), leading to an 
      overall decrease in assay time from 73 min to 32 min. The magnetically-actuated 
      assay also lowered the limit of detection (LOD) for MCP-1 from 124 pg mL(-1) down 
      to 57 pg mL(-1). In sum, the addition of magnetic actuation into bead-enhanced 
      sandwich assays on a silicon photonic biosensor platform might facilitate 
      improved detection of biomarkers in point-of-care diagnostics settings.
FAU - Valera, Enrique
AU  - Valera E
AD  - Department of Chemistry, University of Illinois at Urbana-Champaign 600 South 
      Matthews Avenue, Urbana, IL 61801, USA.
FAU - McClellan, Melinda S
AU  - McClellan MS
AD  - Department of Chemistry, University of Illinois at Urbana-Champaign 600 South 
      Matthews Avenue, Urbana, IL 61801, USA.
FAU - Bailey, Ryan C
AU  - Bailey RC
AD  - Department of Chemistry, University of Illinois at Urbana-Champaign 600 South 
      Matthews Avenue, Urbana, IL 61801, USA.
LA  - eng
GR  - DP2 OD002190/OD/NIH HHS/United States
GR  - R33 CA177462/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20150626
PL  - England
TA  - Anal Methods
JT  - Analytical methods : advancing methods and applications
JID - 101519733
PMC - PMC4627713
MID - NIHMS708402
EDAT- 2015/11/04 06:00
MHDA- 2015/11/04 06:00
PMCR- 2016/10/21
CRDT- 2015/11/04 06:00
PHST- 2015/11/04 06:00 [entrez]
PHST- 2015/11/04 06:00 [pubmed]
PHST- 2015/11/04 06:00 [medline]
PHST- 2016/10/21 00:00 [pmc-release]
AID - 10.1039/C5AY01477H [doi]
PST - ppublish
SO  - Anal Methods. 2015 Oct 21;7(20):8539-8544. doi: 10.1039/C5AY01477H. Epub 2015 Jun 
      26.