PMID- 26529644
OWN - NLM
STAT- MEDLINE
DCOM- 20160919
LR  - 20151218
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1630
DP  - 2016 Jan 1
TI  - Effects of hindlimb unloading on neurotrophins in the rat spinal cord and soleus 
      muscle.
PG  - 1-9
LID - S0006-8993(15)00813-6 [pii]
LID - 10.1016/j.brainres.2015.10.042 [doi]
AB  - The aim of the present study was to investigate the effects of hindlimb unloading 
      (HU) on the expression of neurotrophin-3 (NT-3) and brain-derived neurotrophic 
      factor (BDNF), together with the expression of their high-affinity receptors 
      tropomyosin receptor kinase C (TrkC) and tropomyosin receptor kinase B (TrkB), in 
      lumbar (L4-6) segment of the spinal cord and in the soleus muscle. The mRNA and 
      protein levels of the genes of interest were compared using quantitative PCR and 
      western blot assays. Immunohistochemistry for NT-3 and BDNF was used to detect 
      the levels of protein in the motoneurons in the lateral motor column. In this 
      study, NT-3 and BDNF mRNA and protein expression were significantly increased in 
      the spinal cord and soleus muscle after HU. NT-3 immunoreactivity, but not BDNF 
      immunoreactivity, was significantly increased in the large motoneurons located in 
      lateral motor column after 14 days of HU. The level of TrkC protein in the spinal 
      cord and soleus muscle were significantly elevated after both 7 days and 14 days 
      of HU. However, TrkC mRNA, TrkB mRNA and TrkB protein levels did not change 
      significantly. Elevated BDNF, NT-3 and TrkC levels in the neuromuscular system 
      indicate that neurotrophins are involved in HU-induced neuromuscular plasticity. 
      NT-3 is a candidate to mediate the synaptic efficacy between alpha motoneurons 
      and group Ia afferents.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Yang, Wei
AU  - Yang W
AD  - Department of Physiology, Key Laboratory of Cellular Physiology, Ministry of 
      Education, Shanxi Medical University, Taiyuan, China. Electronic address: 
      vividmail@163.com.
FAU - Zhang, Hao
AU  - Zhang H
AD  - Key Laboratory of Ministry of Education, Shanxi Medical University, Taiyuan, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151031
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (Receptor, trkC)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Hindlimb Suspension/*physiology
MH  - Immunohistochemistry
MH  - Lumbar Vertebrae
MH  - Muscle, Skeletal/*metabolism/pathology
MH  - Neurotrophin 3/metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/metabolism
MH  - Random Allocation
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/metabolism
MH  - Receptor, trkC/metabolism
MH  - Spinal Cord/*metabolism/pathology
OTO - NOTNLM
OT  - Disuse
OT  - Neuromuscular plasticity
OT  - Neurotrophin
OT  - Skeletal muscle
OT  - Spinal cord
EDAT- 2015/11/04 06:00
MHDA- 2016/09/20 06:00
CRDT- 2015/11/04 06:00
PHST- 2015/05/26 00:00 [received]
PHST- 2015/10/15 00:00 [revised]
PHST- 2015/10/25 00:00 [accepted]
PHST- 2015/11/04 06:00 [entrez]
PHST- 2015/11/04 06:00 [pubmed]
PHST- 2016/09/20 06:00 [medline]
AID - S0006-8993(15)00813-6 [pii]
AID - 10.1016/j.brainres.2015.10.042 [doi]
PST - ppublish
SO  - Brain Res. 2016 Jan 1;1630:1-9. doi: 10.1016/j.brainres.2015.10.042. Epub 2015 
      Oct 31.