PMID- 26531772
OWN - NLM
STAT- MEDLINE
DCOM- 20160502
LR  - 20231111
IS  - 1559-4106 (Electronic)
IS  - 1934-8630 (Print)
IS  - 1559-4106 (Linking)
VI  - 11
IP  - 2
DP  - 2015 Jun 3
TI  - Three-dimensional localization of polymer nanoparticles in cells using ToF-SIMS.
PG  - 02A304
LID - 10.1116/1.4934795 [doi]
LID - 02A304
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) three-dimensional (3D) 
      depth profiling and a novel background subtraction method were used to localize 
      polymeric nanoparticles within cells. Results showed that ToF-SIMS 3D depth 
      profiling is capable of localizing polymer nanoparticles within HeLa cells. 
      ToF-SIMS results compared well with optical images of cells incubated with 
      fluorescently labeled polymer nanoparticles, with both imaging techniques 
      demonstrating clustering of nanoparticles in punctate regions consistent with 
      endosomal localization as anticipated based on the nanoparticle design.
FAU - Graham, Daniel J
AU  - Graham DJ
AD  - National ESCA and Surface Analysis Center for Biomedical Problems, Seattle, 
      Washington, 98195 and Department of Bioengineering, University of Washington, 
      Seattle, Washington 98195.
FAU - Wilson, John T
AU  - Wilson JT
AD  - Department of Bioengineering, University of Washington, Seattle, Washington 98195 
      and Department of Chemical and Biomolecular Engineering, Vanderbilt University, 
      Nashville, Tennessee 37212.
FAU - Lai, James J
AU  - Lai JJ
AD  - Department of Bioengineering, University of Washington, Seattle, Washington 
      98195.
FAU - Stayton, Patrick S
AU  - Stayton PS
AD  - Department of Bioengineering, University of Washington, Seattle, Washington 
      98195.
FAU - Castner, David G
AU  - Castner DG
AD  - National ESCA and Surface Analysis Center for Biomedical Problems, Seattle, 
      Washington, 98195; Department of Bioengineering, University of Washington, 
      Seattle, Washington 98195; and Department of Chemical Engineering, University of 
      Washington, Seattle, Washington 98185.
LA  - eng
GR  - P41 EB002027/EB/NIBIB NIH HHS/United States
GR  - EB-002027/EB/NIBIB NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150603
PL  - United States
TA  - Biointerphases
JT  - Biointerphases
JID - 101275679
RN  - 0 (Polymers)
SB  - IM
MH  - Epithelial Cells/*chemistry
MH  - HeLa Cells
MH  - Humans
MH  - Imaging, Three-Dimensional/methods
MH  - Nanoparticles/*analysis/chemistry
MH  - Polymers/*analysis
MH  - Spectrometry, Mass, Secondary Ion/*methods
PMC - PMC4636497
EDAT- 2015/11/05 06:00
MHDA- 2016/05/03 06:00
PMCR- 2016/11/03
CRDT- 2015/11/05 06:00
PHST- 2015/11/05 06:00 [entrez]
PHST- 2015/11/05 06:00 [pubmed]
PHST- 2016/05/03 06:00 [medline]
PHST- 2016/11/03 00:00 [pmc-release]
AID - 1.4934795 [pii]
AID - 304602BIP [pii]
AID - 10.1116/1.4934795 [doi]
PST - epublish
SO  - Biointerphases. 2015 Jun 3;11(2):02A304. doi: 10.1116/1.4934795.