PMID- 26535827
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20191210
IS  - 1942-7522 (Electronic)
IS  - 0145-5613 (Linking)
VI  - 94
IP  - 10-11
DP  - 2015 Oct-Nov
TI  - Effects of different combinations of growth factors on the differentiation of 
      neural stem cells into hair-like cells.
PG  - E23-8
AB  - The differentiation of neural stem cells (NSCs) is influenced by a variety of 
      factors. Therefore, it is important to explore the best external conditions that 
      will induce NSCs to differentiate into hair cells. In this study, we investigated 
      the best in vitro conditions for differentiation of NSCs derived from the 
      hippocampus of newborn guinea pigs into hair-like cells. NSCs were separated and 
      induced in different combinations of growth factors-in a control group and 7 
      combinations. Myosin VIIa-positive cells were detected to compare the effects of 
      various combinations of growth factors on the differentiation of NSCs into 
      hair-like cells. NSCs were differentiated into hair-like cells in all groups, but 
      cell growth was best in the basic fibroblast growth factor (bFGF) + epidermal 
      growth factor (EGF) group and the bFGF + EGF + brain-derived neurotrophic factor 
      (BDNF) group. The rates of myosin VIIa-positive cells in the 8 groups studied 
      ranged from 13.53 to 22.71%, but the results in the bFGF+EGF and bFGF+EGF+BDNF 
      groups had statistical significance compared with other groups (p < 0.05). While 
      bFGF, EGF, and BDNF all can induce the differentiation of NSCs into hair-like 
      cells, the synergies of bFGF+EGF and bFGF+EGF+BDNF are the best.
FAU - Wang, Ying
AU  - Wang Y
AD  - Department of Otolaryngology, the First Affiliated Hospital of Zhengzhou 
      University, Number One, Constructive East Road, Zhengzhou City 450052, China.
FAU - Dong, Ming-min
AU  - Dong MM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ear Nose Throat J
JT  - Ear, nose, & throat journal
JID - 7701817
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Myosin VIIa)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 3.6.4.1 (Myosins)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Differentiation/*drug effects
MH  - Cell Enlargement
MH  - Cells, Cultured
MH  - Epidermal Growth Factor/*pharmacology
MH  - Fibroblast Growth Factor 2/*pharmacology
MH  - Guinea Pigs
MH  - Hair Cells, Auditory/cytology
MH  - Hippocampus/cytology
MH  - Microscopy, Fluorescence
MH  - Myosin VIIa
MH  - Myosins/analysis
MH  - Neural Stem Cells/*cytology/drug effects
EDAT- 2015/11/05 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/11/05 06:00
PHST- 2015/11/05 06:00 [entrez]
PHST- 2015/11/05 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PST - ppublish
SO  - Ear Nose Throat J. 2015 Oct-Nov;94(10-11):E23-8.