PMID- 26536760
OWN - NLM
STAT- MEDLINE
DCOM- 20151114
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 33
IP  - 6
DP  - 2015 Jun
TI  - [Determination of anthocyanins in the peel of sweet cherry by ultra performance 
      liquid chromatography tandem mass spectrometry].
PG  - 577-82
AB  - A method for the determination of seven anthocyanins in the peel of sweet cherry 
      was developed by ultra performance liquid chromatography-tandem mass spectrometry 
      (UPLC-MS/MS). The sample was extracted by methanol containing 0.1% (v/v) 
      hydrochloric acid, and then purified by AB-8 macroporous resins. The separation 
      was carried out on a Phenomenex Kinetex column (100 mm x 4.6 mm, 2.6 Rm) with 
      mobile phase of 0.1% (v/v) formic acid aqueous solution containing 5 mmol/L 
      ammonium formate and methanol. The sample solution was detected by UPLC-MS/MS 
      with ESI under positive ion and multi reaction monitoring (MRM) modes. The 
      results showed that the limits of quantification (LOQs) for the seven target 
      compounds were 0.26-1.42 microg/kg. The seven anthocyanin standards showed a good 
      linearity in the range of 0-100 microg/L with the correlation coefficients (r2) of 
      0.996 4-0.999 3. The average recoveries of the seven anthocyanins were 
      97.2%-105.4%, and the relative standard deviations (RSDs) were 1.9%-5.8%. The 
      mature fruit samples of sweet cherry red variety "Tieton" and the yellow variety 
      "13-33" were analyzed by this method. The results showed that the anthocyanin 
      composition and contents were significantly different between the two varieties. 
      This method can be used for rapid identification and the determination of 
      anthocyanin components in sweet cherry fruits due to its simple operation, high 
      sensitivity, good reproducibility and covering a wide range of anthocyanins.
FAU - Wei, Hairong
AU  - Wei H
FAU - Yi, Xibin
AU  - Yi X
FAU - Tan, Yue
AU  - Tan Y
FAU - Zong, Xiaojuan
AU  - Zong X
FAU - Wang, Jiawei
AU  - Wang J
FAU - Xu, Li
AU  - Xu L
FAU - Liu, Qingzhong
AU  - Liu Q
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Anthocyanins)
SB  - IM
MH  - Anthocyanins/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Fruit/*chemistry
MH  - Prunus/*chemistry
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry
EDAT- 2015/11/06 06:00
MHDA- 2015/11/15 06:00
CRDT- 2015/11/06 06:00
PHST- 2015/11/06 06:00 [entrez]
PHST- 2015/11/06 06:00 [pubmed]
PHST- 2015/11/15 06:00 [medline]
AID - 10.3724/sp.j.1123.2015.01020 [doi]
PST - ppublish
SO  - Se Pu. 2015 Jun;33(6):577-82. doi: 10.3724/sp.j.1123.2015.01020.