PMID- 26536775
OWN - NLM
STAT- MEDLINE
DCOM- 20160211
LR  - 20151105
IS  - 0047-1860 (Print)
IS  - 0047-1860 (Linking)
VI  - 63
IP  - 4
DP  - 2015 Apr
TI  - [Factors Affecting Non-Specific Reaction in Latex Agglutination Turbidimetric 
      Immunoassay for Detection of Matrix Metalloproteinase-3].
PG  - 427-34
AB  - PURPOSE: Measurement of matrix metalloproteinase-3(MMP-3), a marker for 
      rheumatoid arthritis, by means of latex agglutination turbidimetric immunoassay 
      (LTIA), has come to replace ELISA kit, but pre-improvement LTIA kit S(Sekisui 
      Medical) frequently shows false values resulting from non-specific reactions. We 
      analyzed factors influencing the frequency of non-specific reactions for MMP-3 
      detection using various methods. METHOD: Serum MMP-3 levels were measured in 
      1,214 routine samples or 57 panels with various immunoserological abnormalities 
      by means of pre-improvement and improved kit S, LTIA kit E (Eiken Chemical) and 
      ELISA kit. Non-specific reaction samples were selected either from the routine 
      samples based on the results of correlativity tests between the kits and of 
      dilution linearity tests, or from 57 panels with immunoserological abnormalities 
      based on the results of recovery tests. To explore the factors causing non- 
      specific reactions, titers of rheumatoid factor (RF), immunoglobulins and four 
      heterophil antibodies were measured in these samples. In addition, changes in 
      findings as a result of reduction treatment or IgM absorption were examined in 
      selected samples to clarify the effect of heterophil antibodies. RESULTS: Samples 
      which showed suspected false values probably due to non-specific reaction 
      numbered 75, 15, 5 and 17 as measured with pre-improvement kit S, improved kit S, 
      kit E and ELISA kit, respectively. Kit S also showed high rate of deviation in 
      recovery tests on panels with immunoserological abnormalities. Rate of RF titer 
      in samples with non-specific reactions was higher than that of other factors. 
      Non-specific reaction could be inhibited in two samples as a result of reduction 
      treatment or absorption of IgM subtype. CONCLUSION: Pre-improvement kit S showed 
      a higher rate of non-specific reactions than other kits. The most common cause of 
      non-specific reactions is thought to be high RF titer, but high levels of IgM, 
      including heterophil antibodies, may also affect the values of MMP-3.
FAU - Kitaaki, Shoko
AU  - Kitaaki S
FAU - Hayashi, Nobuhide
AU  - Hayashi N
FAU - Sato, Itsuko
AU  - Sato I
FAU - Watanabe, Katsunori
AU  - Watanabe K
FAU - Saegusa, Jun
AU  - Saegusa J
FAU - Kawano, Seiji
AU  - Kawano S
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Rinsho Byori
JT  - Rinsho byori. The Japanese journal of clinical pathology
JID - 2984781R
RN  - 0 (Antibodies, Heterophile)
RN  - 0 (Biomarkers)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - 9009-79-4 (Rheumatoid Factor)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Antibodies, Heterophile
MH  - Arthritis, Rheumatoid/diagnosis
MH  - Biomarkers/blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoassay/*methods
MH  - Immunoglobulin M
MH  - Latex Fixation Tests/*methods
MH  - Matrix Metalloproteinase 3/*blood
MH  - Nephelometry and Turbidimetry/*methods
MH  - *Reagent Kits, Diagnostic
MH  - Rheumatoid Factor
EDAT- 2015/11/06 06:00
MHDA- 2016/02/13 06:00
CRDT- 2015/11/06 06:00
PHST- 2015/11/06 06:00 [entrez]
PHST- 2015/11/06 06:00 [pubmed]
PHST- 2016/02/13 06:00 [medline]
PST - ppublish
SO  - Rinsho Byori. 2015 Apr;63(4):427-34.