PMID- 26536910
OWN - NLM
STAT- MEDLINE
DCOM- 20160509
LR  - 20190402
IS  - 0304-4920 (Print)
IS  - 0304-4920 (Linking)
VI  - 58
IP  - 5
DP  - 2015 Oct 31
TI  - Beneficial Effects of Hypoxic Preconditioning on Human Umbilical Cord Mesenchymal 
      Stem Cells.
PG  - 343-53
LID - CJP.2015.BAE369 [pii]
LID - 10.4077/CJP.2015.BAE369 [doi]
AB  - As human umbilical cord mesenchymal stem cells (hUC-MSCs) transplanation may be 
      promising in heart failure treatment, it is important to know whether hypoxic 
      preconditioning (HP) promote hUC-MSCs proliferation and differentiation and 
      protect them against chemical hypoxic damages. This study aimed to investigate 
      the effects of HP on proliferation and differentiation of human umbilical cord 
      mesenchymal stem cells (hUC-MSCs). The study also aimed to confirm our hypothesis 
      that HP could promote hUC-MSCs proliferation and differentiation to 
      cardiomyocyte-like cells as well as effectively protecting hUC-MSCs and 
      cardiomyocyte-like cells against chemical hypoxic damages. Isolated hUC-MSCs were 
      cultured in hypoxia at 1%, 3% and 5% O(2) for 72 hours. 5-azacytidine (5-AZA) 
      induced differentiation of hUC-MSCs to cardiomyocyte-like cells was determined by 
      streptavidin-perosidase (SP) immunohistochemical staining and the content of 
      troponin (TnI). Flow cytometry was used to measure cell cycle in hUC-MSCs and 
      cardiomyocyte-like cells. The mitochondrial membrane potential (DeltaPsi(m)) and 
      mitochondrial Ca(2)(+) concentration ([Ca(2)(+)](m)), were measured in hUC-MSCs and 
      cardiomyocyte-like cells during chemical hypoxia induced by cobalt chloride (100 
      mumol/L). HP optimally promoted the proliferation of hUC-MSCs at 3% O(2) and 
      enhanced the differentiation of hUC-MSCs to cardiomyocyte-like cells by 5-AZA in 
      a concentration-dependent manner. The cell cycle distribution of 
      cardiomyocyte-like cells, but not hUC-MSCs, was clearly changed by HP. Chemical 
      hypoxic damage, decreased DeltaPsi(m) and increased [Ca(2)(+)](m), were alleviated 
      significantly in HP-treated cells compared with the normaxia-treated cells. The 
      results demonstrate that HP promoted hUC-MSCs proliferation and differentiation 
      to cardiomyocyte-like cells, and protected both cell types against chemical 
      hypoxic damage.
FAU - Zhang, Li
AU  - Zhang L
AD  - Department of Physiology, Hebei Medical University, Shijiazhuang 050017, Hebei, 
      People's Republic of China.
AD  - Department of Cardiology, Bethune International Peace Hospital, Shijiazhuang 
      050082, Hebei, People's Republic of China.
FAU - Yang, Jing
AU  - Yang J
AD  - Department of Physiology, Hebei Medical University, Shijiazhuang 050017, Hebei, 
      People's Republic of China.
FAU - Tian, Yan-Ming
AU  - Tian YM
AD  - Department of Physiology, Hebei Medical University, Shijiazhuang 050017, Hebei, 
      People's Republic of China.
FAU - Guo, Hui
AU  - Guo H
AD  - Department of Gynaecology and Obstetrics, the Fourth Hospital, Hebei Medical 
      University, Shijiazhuang 050000, Hebei, People's Republic of China.
FAU - Zhang, Yi
AU  - Zhang Y
AD  - Department of Physiology, Hebei Medical University, Shijiazhuang 050017, Hebei, 
      People's Republic of China.
AD  - Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease, 
      Shijiazhuang 050000, Hebei, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Chin J Physiol
JT  - The Chinese journal of physiology
JID - 7804502
SB  - IM
MH  - Cell Cycle
MH  - *Cell Differentiation
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - Humans
MH  - *Hypoxia
MH  - Ischemic Preconditioning
MH  - Mesenchymal Stem Cells/*physiology
MH  - Myocytes, Cardiac/*ultrastructure
EDAT- 2015/11/06 06:00
MHDA- 2016/05/10 06:00
CRDT- 2015/11/06 06:00
PHST- 2015/11/06 06:00 [entrez]
PHST- 2015/11/06 06:00 [pubmed]
PHST- 2016/05/10 06:00 [medline]
AID - CJP.2015.BAE369 [pii]
AID - 10.4077/CJP.2015.BAE369 [doi]
PST - ppublish
SO  - Chin J Physiol. 2015 Oct 31;58(5):343-53. doi: 10.4077/CJP.2015.BAE369.