PMID- 26537366
OWN - NLM
STAT- MEDLINE
DCOM- 20160923
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 5
DP  - 2015 Nov 5
TI  - Wnt/beta-catenin coupled with HIF-1alpha/VEGF signaling pathways involved in galangin 
      neurovascular unit protection from focal cerebral ischemia.
PG  - 16151
LID - 10.1038/srep16151 [doi]
LID - 16151
AB  - Microenvironmental regulation has become a promising strategy for complex disease 
      treatment. The neurovascular unit (NVU), as the key structural basis to maintain 
      an optimal brain microenvironment, has emerged as a new paradigm to understand 
      the pathology of stroke. In this study, we investigated the effects of galangin, 
      a natural flavonoid isolated from the rhizome of Alpina officinarum Hance, on NVU 
      microenvironment improvement and associated signal pathways in rats impaired by 
      middle cerebral artery occlusion (MCAO). Galangin ameliorated neurological 
      scores, cerebral infarct volume and cerebral edema and reduced the concentration 
      of Evans blue (EB) in brain tissue. NVU ultrastructural changes were also 
      improved by galangin. RT-PCR and western blot revealed that galangin protected 
      NVUs through the Wnt/beta-catenin pathway coupled with HIF-1alpha and vascular 
      endothelial growth factor (VEGF). VEGF and beta-catenin could be the key nodes of 
      these two coupled pathways. In conclusion, Galangin might function as an 
      anti-ischemic stroke drug by improving the microenvironment of NVUs.
FAU - Wu, Chuanhong
AU  - Wu C
AD  - Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 
      Beijing 100700, China.
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, 999078, China.
FAU - Chen, Jianxin
AU  - Chen J
AD  - Beijing University of Chinese Medicine, Beijing 100029, China.
FAU - Chen, Chang
AU  - Chen C
AD  - Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 
      Beijing 100700, China.
FAU - Wang, Wei
AU  - Wang W
AD  - Beijing University of Chinese Medicine, Beijing 100029, China.
FAU - Wen, Limei
AU  - Wen L
AD  - The first Affiliated Hospital of Xinjiang Medical University, Xinjiang, 830054, 
      China.
FAU - Gao, Kuo
AU  - Gao K
AD  - Beijing University of Chinese Medicine, Beijing 100029, China.
FAU - Chen, Xiuping
AU  - Chen X
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, 999078, China.
FAU - Xiong, Sihuai
AU  - Xiong S
AD  - Beijing No.166 High School, Beijing 100006, China.
FAU - Zhao, Huihui
AU  - Zhao H
AD  - Beijing University of Chinese Medicine, Beijing 100029, China.
FAU - Li, Shaojing
AU  - Li S
AD  - Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 
      Beijing 100700, China.
AD  - Beijing University of Chinese Medicine, Beijing 100029, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151105
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Flavonoids)
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Plant Extracts)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (beta Catenin)
RN  - 0 (vascular endothelial growth factor A, rat)
RN  - 142FWE6ECS (galangin)
SB  - IM
MH  - Animals
MH  - Brain/drug effects/metabolism
MH  - Brain Ischemia/*drug therapy/metabolism
MH  - Flavonoids/*pharmacology
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism
MH  - Infarction, Middle Cerebral Artery/drug therapy/metabolism
MH  - Male
MH  - Neuroprotective Agents/*pharmacology
MH  - Plant Extracts/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
MH  - Vascular Endothelial Growth Factor A/*metabolism
MH  - Wnt Signaling Pathway/*drug effects
MH  - beta Catenin/*metabolism
PMC - PMC4633613
EDAT- 2015/11/06 06:00
MHDA- 2016/09/24 06:00
PMCR- 2015/11/05
CRDT- 2015/11/06 06:00
PHST- 2015/01/09 00:00 [received]
PHST- 2015/06/17 00:00 [accepted]
PHST- 2015/11/06 06:00 [entrez]
PHST- 2015/11/06 06:00 [pubmed]
PHST- 2016/09/24 06:00 [medline]
PHST- 2015/11/05 00:00 [pmc-release]
AID - srep16151 [pii]
AID - 10.1038/srep16151 [doi]
PST - epublish
SO  - Sci Rep. 2015 Nov 5;5:16151. doi: 10.1038/srep16151.