PMID- 26537835
OWN - NLM
STAT- MEDLINE
DCOM- 20160923
LR  - 20220311
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 5
DP  - 2015 Nov 5
TI  - 2A self-cleaving peptide-based multi-gene expression system in the silkworm 
      Bombyx mori.
PG  - 16273
LID - 10.1038/srep16273 [doi]
LID - 16273
AB  - Fundamental and applied studies of silkworms have entered the functional genomics 
      era. Here, we report a multi-gene expression system (MGES) based on 2A 
      self-cleaving peptide (2A), which regulates the simultaneous expression and 
      cleavage of multiple gene targets in the silk gland of transgenic silkworms. 
      First, a glycine-serine-glycine spacer (GSG) was found to significantly improve 
      the cleavage efficiency of 2A. Then, the cleavage efficiency of six types of 2As 
      with GSG was analyzed. The shortest porcine teschovirus-1 2A (P2A-GSG) exhibited 
      the highest cleavage efficiency in all insect cell lines that we tested. Next, 
      P2A-GSG successfully cleaved the artificial human serum albumin (66 kDa) linked 
      with human acidic fibroblast growth factor (20.2 kDa) fusion genes and 
      vitellogenin receptor fragment (196 kD) of silkworm linked with EGFP fusion 
      genes, importantly, vitellogenin receptor protein was secreted to the outside of 
      cells. Furthermore, P2A-GSG successfully mediated the simultaneous expression and 
      cleavage of a DsRed and EGFP fusion gene in silk glands and caused secretion into 
      the cocoon of transgenic silkworms using our sericin1 expression system. We 
      predicted that the MGES would be an efficient tool for gene function research and 
      innovative research on various functional silk materials in medicine, cosmetics, 
      and other biomedical areas.
FAU - Wang, Yuancheng
AU  - Wang Y
AD  - State key laboratory of silkworm genome biology, Southwest University, Chongqing, 
      China.
AD  - College of biology and technology, Southwest University, Chongqing, China.
FAU - Wang, Feng
AU  - Wang F
AD  - State key laboratory of silkworm genome biology, Southwest University, Chongqing, 
      China.
AD  - College of biology and technology, Southwest University, Chongqing, China.
FAU - Wang, Riyuan
AU  - Wang R
AD  - State key laboratory of silkworm genome biology, Southwest University, Chongqing, 
      China.
FAU - Zhao, Ping
AU  - Zhao P
AD  - State key laboratory of silkworm genome biology, Southwest University, Chongqing, 
      China.
AD  - College of biology and technology, Southwest University, Chongqing, China.
FAU - Xia, Qingyou
AU  - Xia Q
AD  - State key laboratory of silkworm genome biology, Southwest University, Chongqing, 
      China.
AD  - College of biology and technology, Southwest University, Chongqing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151105
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Egg Proteins)
RN  - 0 (Peptides)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Silk)
RN  - 0 (vitellogenin receptor)
RN  - 62031-54-3 (Fibroblast Growth Factors)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified/genetics/metabolism
MH  - Bombyx/*genetics/*metabolism
MH  - Cell Line
MH  - Egg Proteins/genetics/metabolism
MH  - Fibroblast Growth Factors/genetics/metabolism
MH  - Gene Expression/*genetics
MH  - Genetic Vectors/genetics
MH  - Humans
MH  - Peptides/*genetics/*metabolism
MH  - Receptors, Cell Surface/genetics/metabolism
MH  - Silk/genetics/metabolism
PMC - PMC4633692
EDAT- 2015/11/06 06:00
MHDA- 2016/09/24 06:00
PMCR- 2015/11/05
CRDT- 2015/11/06 06:00
PHST- 2015/05/05 00:00 [received]
PHST- 2015/10/07 00:00 [accepted]
PHST- 2015/11/06 06:00 [entrez]
PHST- 2015/11/06 06:00 [pubmed]
PHST- 2016/09/24 06:00 [medline]
PHST- 2015/11/05 00:00 [pmc-release]
AID - srep16273 [pii]
AID - 10.1038/srep16273 [doi]
PST - epublish
SO  - Sci Rep. 2015 Nov 5;5:16273. doi: 10.1038/srep16273.