PMID- 26538054
OWN - NLM
STAT- MEDLINE
DCOM- 20161006
LR  - 20161230
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 180
DP  - 2016 Feb
TI  - Apoptosis-like cell death induced by nematocyst venom from Chrysaora helvola 
      Brandt jellyfish and an in vitro evaluation of commonly used antidotes.
PG  - 31-9
LID - S1532-0456(15)00143-X [pii]
LID - 10.1016/j.cbpc.2015.10.012 [doi]
AB  - The present work investigated the in vitro cytotoxicity of nematocyst venom (NV) 
      from Chrysaora helvola Brandt (C. helvola) jellyfish against human MCF-7 and 
      CNE-2 tumor cell lines. Potent cytotoxicity was quantified using the MTT assay 
      (LC50=12.07+/-3.13 and 1.6+/-0.22mug/mL (n=4), respectively). Apoptosis-like cell 
      death was further confirmed using the LDH release assay and Annexin V/PI double 
      staining-based flow cytometry analysis. However, only activation of caspase-4 was 
      observed. It is possible that some caspase-independent pathways were activated by 
      the NV treatment. Since no reference or antivenom is available, the effects of 
      several commonly used antidotes on the cytotoxicity of NV were examined on more 
      sensitive CNE-2 cells to determine the appropriate emergency measures for 
      envenomation by C. helvola. The phospholipase A2 (PLA2) inhibitor 
      para-bromophenacyl bromide (pBPB) showed no protective effect, while Mg(2+) 
      potentiated cytotoxicity. Voltage-gated L-type Ca(2+) channel blockers 
      (verapamil, nifedipine and felodipine) and Na-Ca(2+) exchanger inhibitor KB-R7943 
      also showed no effect. Assays using Ca(2+)-free culture media or the 
      intracellular Ca(2+) chelator BAPTA also could not inhibit the cytotoxicity. 
      Taken together, these results suggest that PLA2 and Ca(2+) are not directly 
      involved in the cytotoxicity of NV from C. helvola. Our work also suggests 
      caution regarding the choice for first aid for envenomation by C. helvola 
      jellyfish.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Qu, Xiaosheng
AU  - Qu X
AD  - Engineering Lab for Endangered Medicinal Resources of Southwest China, Guangxi 
      Medicinal Herb Garden, Nanning 530023, China. Electronic address: 
      239653129@qq.com.
FAU - Xia, Xianghua
AU  - Xia X
AD  - Engineering Lab for Endangered Medicinal Resources of Southwest China, Guangxi 
      Medicinal Herb Garden, Nanning 530023, China.
FAU - Lai, Zefeng
AU  - Lai Z
AD  - Department of Pharmacology, Guangxi Medicinal University, Nanning 530021, China.
FAU - Zhong, Taozheng
AU  - Zhong T
AD  - Engineering Lab for Endangered Medicinal Resources of Southwest China, Guangxi 
      Medicinal Herb Garden, Nanning 530023, China.
FAU - Li, Gang
AU  - Li G
AD  - Engineering Lab for Endangered Medicinal Resources of Southwest China, Guangxi 
      Medicinal Herb Garden, Nanning 530023, China.
FAU - Fan, Lanlan
AU  - Fan L
AD  - Guangxi University of Traditional Chinese Medicine, Nanning, 530200, China.
FAU - Shu, Wei
AU  - Shu W
AD  - Department of Cell Biology and Genetics, Guangxi Medicinal University, Nanning 
      530021, China. Electronic address: shuwei7866@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151030
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 0 (Antidotes)
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Calcium Chelating Agents)
RN  - 0 (Cnidarian Venoms)
RN  - EC 3.4.22.- (Caspases)
RN  - I38ZP9992A (Magnesium)
SB  - IM
MH  - Animals
MH  - Antidotes/*pharmacology
MH  - *Apoptosis
MH  - Calcium Channel Blockers/*pharmacology
MH  - Calcium Chelating Agents/*pharmacology
MH  - Caspases
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Cnidarian Venoms/*toxicity
MH  - Humans
MH  - Magnesium
MH  - Scyphozoa/*physiology
OTO - NOTNLM
OT  - Antidote
OT  - Apoptosis
OT  - Ca(2+) channel blockers
OT  - Chrysaora helvola
OT  - Cytotoxicity
OT  - Nematocyst venom
OT  - PLA(2)
EDAT- 2015/11/06 06:00
MHDA- 2016/10/08 06:00
CRDT- 2015/11/06 06:00
PHST- 2015/07/17 00:00 [received]
PHST- 2015/10/26 00:00 [revised]
PHST- 2015/10/28 00:00 [accepted]
PHST- 2015/11/06 06:00 [entrez]
PHST- 2015/11/06 06:00 [pubmed]
PHST- 2016/10/08 06:00 [medline]
AID - S1532-0456(15)00143-X [pii]
AID - 10.1016/j.cbpc.2015.10.012 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2016 Feb;180:31-9. doi: 
      10.1016/j.cbpc.2015.10.012. Epub 2015 Oct 30.