PMID- 26539042 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20151105 LR - 20200930 IS - 1229-8093 (Print) IS - 2092-9323 (Electronic) IS - 1229-8093 (Linking) VI - 43 IP - 3 DP - 2015 Sep TI - Isolation and Identification of Yeasts from Wild Flowers Collected around Jangseong Lake in Jeollanam-do, Republic of Korea, and Characterization of the Unrecorded Yeast Bullera coprosmaensis. PG - 266-71 LID - 10.5941/MYCO.2015.43.3.266 [doi] AB - Several types of yeasts were isolated from wild flowers around Jangseong Lake in Jeollanam-do, Republic of Korea and identified by comparing the nucleotide sequences of the PCR amplicons for the D1/D2 variable domain of the 26S ribosomal DNA using Basic Local Alignment Search Tool (BLAST) analysis. In total, 60 strains from 18 species were isolated, and Pseudozyma spp. (27 strains), which included Pseudozyma rugulosa (7 strains) and Pseudozyma aphidis (6 strains), was dominant species. Among the 60 strains, Bullera coprosmaensis JS00600 represented a newly recorded yeast strain in Korea, and its microbiological characteristics were investigated. The yeast cell has an oval-shaped morphology measuring 1.4 x 1.7 microm in size. Bullera coprosmaensis JS00600 is an asporous yeast that exhibits no pseudomycelium formation. It grew well in vitamin-free medium as well as in yeast extract-malt extract broth and yeast extract-peptone-dextrose (YPD) broth, and it is halotolerant growing in 10% NaCl-containing YPD broth. FAU - Han, Sang-Min AU - Han SM AD - Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon 35345, Korea. FAU - Hyun, Se-Hee AU - Hyun SH AD - Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon 35345, Korea. FAU - Lee, Hyang Burm AU - Lee HB AD - Division of Food Technology, Biotechnology and Agrochemistry, Chonnam National University, Gwangju 61186, Korea. FAU - Lee, Hye Won AU - Lee HW AD - Division of Food Technology, Biotechnology and Agrochemistry, Chonnam National University, Gwangju 61186, Korea. FAU - Kim, Ha-Kun AU - Kim HK AD - Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon 35345, Korea. FAU - Lee, Jong-Soo AU - Lee JS AD - Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon 35345, Korea. LA - eng PT - Journal Article DEP - 20150930 PL - Korea (South) TA - Mycobiology JT - Mycobiology JID - 100960027 PMC - PMC4630432 OTO - NOTNLM OT - Bullera coprosmaensis OT - Characteristics OT - Jangseong Lake OT - Unrecorded yeast OT - Wild flowers EDAT- 2015/11/06 06:00 MHDA- 2015/11/06 06:01 PMCR- 2015/09/01 CRDT- 2015/11/06 06:00 PHST- 2015/07/16 00:00 [received] PHST- 2015/09/08 00:00 [revised] PHST- 2015/09/08 00:00 [accepted] PHST- 2015/11/06 06:00 [entrez] PHST- 2015/11/06 06:00 [pubmed] PHST- 2015/11/06 06:01 [medline] PHST- 2015/09/01 00:00 [pmc-release] AID - 10.5941/MYCO.2015.43.3.266 [doi] PST - ppublish SO - Mycobiology. 2015 Sep;43(3):266-71. doi: 10.5941/MYCO.2015.43.3.266. Epub 2015 Sep 30.