PMID- 26539490
OWN - NLM
STAT- MEDLINE
DCOM- 20160818
LR  - 20240325
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2015
DP  - 2015
TI  - Low-Molecular-Weight Polyethyleneimine Grafted Polythiophene for Efficient siRNA 
      Delivery.
PG  - 406389
LID - 10.1155/2015/406389 [doi]
LID - 406389
AB  - Owing to its hydrophilicity, negative charge, small size, and labile degradation 
      by endogenous nucleases, small interfering RNA (siRNA) delivery must be achieved 
      by a carrier system. In this study, cationic copolymers composed of 
      low-molecular-weight polyethylenimine and polythiophenes were synthesized and 
      evaluated as novel self-tracking siRNA delivery vectors. The concept underlying 
      the design of these copolymers is that hydrophobicity and rigidity of 
      polythiophenes should enhance the transport of siRNA across the cell membrane and 
      endosomal membrane. A gel retardation assay showed that the nanosized complexes 
      formed between the copolymers and siRNA were stable even at a molar ratio of 1 : 
      2. The high cellular uptake (>80%) and localization of the copolymer vectors 
      inside the cells were easily analyzed by tracking the fluorescence of 
      polythiophene using fluorescent microscopy and cytometry. An in vitro luciferase 
      knockdown (KD) assay in A549-luc cells demonstrated that the siRNA complexes with 
      more hydrophobic copolymers achieved a higher KD efficiency of 52.8% without 
      notable cytotoxicity, indicating protein-specific KD activity rather than solely 
      the cytotoxicity of the materials. Our polythiophene copolymers should serve as 
      novel, efficient, low cell toxicity, and label-free siRNA delivery systems.
FAU - He, Pan
AU  - He P
AD  - Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, 
      Japan.
FAU - Hagiwara, Kyoji
AU  - Hagiwara K
AD  - Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, 
      Japan.
FAU - Chong, Hui
AU  - Chong H
AD  - Responsive Organic Materials Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 
      351-0198, Japan.
FAU - Yu, Hsiao-hua
AU  - Yu HH
AD  - Responsive Organic Materials Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 
      351-0198, Japan ; Institute of Chemistry, Academia Sinica, 128 Academic Road, 
      Section 2, Nankang, Taipei 11529, Taiwan.
FAU - Ito, Yoshihiro
AU  - Ito Y
AD  - Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, 
      Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151011
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Cations)
RN  - 0 (Polymers)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Thiophenes)
RN  - 25233-34-5 (polythiophene)
RN  - 9002-98-6 (Polyethyleneimine)
SB  - IM
MH  - Cations/administration & dosage/chemistry
MH  - Cell Line
MH  - Electrophoretic Mobility Shift Assay
MH  - *Gene Transfer Techniques
MH  - Genetic Vectors
MH  - Humans
MH  - Molecular Weight
MH  - Polyethyleneimine/*administration & dosage/chemistry
MH  - Polymers/*administration & dosage/chemistry
MH  - RNA, Small Interfering/administration & dosage/*genetics
MH  - Thiophenes/*administration & dosage/chemistry
MH  - Transfection
PMC - PMC4619845
EDAT- 2015/11/06 06:00
MHDA- 2016/08/19 06:00
PMCR- 2015/10/11
CRDT- 2015/11/06 06:00
PHST- 2015/04/04 00:00 [received]
PHST- 2015/06/13 00:00 [revised]
PHST- 2015/06/23 00:00 [accepted]
PHST- 2015/11/06 06:00 [entrez]
PHST- 2015/11/06 06:00 [pubmed]
PHST- 2016/08/19 06:00 [medline]
PHST- 2015/10/11 00:00 [pmc-release]
AID - 10.1155/2015/406389 [doi]
PST - ppublish
SO  - Biomed Res Int. 2015;2015:406389. doi: 10.1155/2015/406389. Epub 2015 Oct 11.