PMID- 26539518
OWN - NLM
STAT- MEDLINE
DCOM- 20160826
LR  - 20181113
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2015
DP  - 2015
TI  - Effect of Laryngeal Squamous Cell Carcinoma Tissue Implantation on the Chick 
      Embryo Chorioallantoic Membrane: Morphometric Measurements and Vascularity.
PG  - 629754
LID - 10.1155/2015/629754 [doi]
LID - 629754
AB  - BACKGROUND: The aim of this study was to develop chick embryo chorioallantoic 
      membrane (CAM) model of laryngeal squamous cell carcinoma (LSCC) and to evaluate 
      the morphological and morphometric characteristics and angiogenic features of it. 
      METHODS: Fresh LSCC tissue samples obtained from 6 patients were implanted onto 
      15 chick embryo CAMs. Morphological, morphometric, and angiogenic changes in the 
      CAM and chorionic epithelium were evaluated up to 4 days after the tumor 
      implantation. Immunohistochemical analysis (34betaE12, CD31, and Ki67 staining) was 
      performed to detect cytokeratins and tumor endothelial cells and to evaluate the 
      proliferative capacity of the tumor before and after implantation on the CAM. 
      RESULTS: The implanted LSCC tissue samples survived on the CAM in all the 
      experiments and retained the essential morphologic characteristics and 
      proliferative capacity of the original tumor. Implants induced thickening of both 
      the CAM (103-417%, p = 0.0001) and the chorionic epithelium (70-140%, p = 0.0001) 
      and increase in number of blood vessels (75-148%, p = 0.0001) in the CAM. 
      CONCLUSIONS: This study clarifies that chick embryo CAM is a relevant assay for 
      implanting LSCC tissue and provides the first morphological and morphometric 
      characterization of the LSCC CAM model that opens new perspectives to study this 
      disease.
FAU - Uloza, Virgilijus
AU  - Uloza V
AD  - Department of Otorhinolaryngology, Lithuanian University of Health Sciences, 
      LT-50009 Kaunas, Lithuania.
FAU - Kuzminiene, Alina
AU  - Kuzminiene A
AD  - Department of Otorhinolaryngology, Lithuanian University of Health Sciences, 
      LT-50009 Kaunas, Lithuania.
FAU - Salomskaite-Davalgiene, Sonata
AU  - Salomskaite-Davalgiene S
AD  - Department of Histology and Embryology, Lithuanian University of Health Sciences, 
      LT-50009 Kaunas, Lithuania.
FAU - Palubinskiene, Jolita
AU  - Palubinskiene J
AD  - Department of Histology and Embryology, Lithuanian University of Health Sciences, 
      LT-50009 Kaunas, Lithuania.
FAU - Balnyte, Ingrida
AU  - Balnyte I
AD  - Department of Histology and Embryology, Lithuanian University of Health Sciences, 
      LT-50009 Kaunas, Lithuania.
FAU - Uloziene, Ingrida
AU  - Uloziene I
AD  - Department of Otorhinolaryngology, Lithuanian University of Health Sciences, 
      LT-50009 Kaunas, Lithuania.
FAU - Saferis, Viktoras
AU  - Saferis V
AD  - Department of Physics, Mathematics and Biophysics, Lithuanian University of 
      Health Sciences, LT-50009 Kaunas, Lithuania.
FAU - Valanciute, Angelija
AU  - Valanciute A
AD  - Department of Histology and Embryology, Lithuanian University of Health Sciences, 
      LT-50009 Kaunas, Lithuania.
LA  - eng
PT  - Journal Article
DEP - 20151011
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
SB  - IM
MH  - Animals
MH  - Carcinoma, Squamous Cell/*pathology
MH  - Chick Embryo
MH  - Chorioallantoic Membrane/*growth & development/pathology
MH  - Chorion/pathology
MH  - Humans
MH  - Laryngeal Neoplasms/*pathology
MH  - *Neovascularization, Pathologic
PMC - PMC4619851
EDAT- 2015/11/06 06:00
MHDA- 2016/08/27 06:00
PMCR- 2015/10/11
CRDT- 2015/11/06 06:00
PHST- 2015/07/10 00:00 [received]
PHST- 2015/09/09 00:00 [revised]
PHST- 2015/09/10 00:00 [accepted]
PHST- 2015/11/06 06:00 [entrez]
PHST- 2015/11/06 06:00 [pubmed]
PHST- 2016/08/27 06:00 [medline]
PHST- 2015/10/11 00:00 [pmc-release]
AID - 10.1155/2015/629754 [doi]
PST - ppublish
SO  - Biomed Res Int. 2015;2015:629754. doi: 10.1155/2015/629754. Epub 2015 Oct 11.