PMID- 26540984
OWN - NLM
STAT- MEDLINE
DCOM- 20160712
LR  - 20181202
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 29
IP  - 7
DP  - 2015 Jul
TI  - [DIFFERENTIATION OF HUMAN UMBILICAL CORD MESENCHYMAL STEM CELLS INTO HEPATOCYTES 
      INDUCED BY RAT FIBROTIC LIVER TISSUE EXTRACTS].
PG  - 878-83
AB  - OBJECTIVE: To investigate the differentiation potential of human umbilical cord 
      mesenchymal stem cells (HUCMSCs) into hepatocytes induced by rat fibrotic liver 
      tissue extracts. METHODS: Liver fibrosis was induced in the Sprague Dawley rats 
      (weighting, 180-220 g) by repeated intraperitoneal injections of 3% 
      thioacetamide-saline at a dose of 200 mg/kg twice a week for 4 weeks; fibrotic 
      liver tissues were used to prepare liver homogenate supernatants. The HUCMSCs at 
      passage 3 were cultured in DMEM/F12 with 10% fetal bovine serum (FBS) (control 
      group) and in DMEM/F12 with 10% FBS and 50 g/L liver homogenate supernatants 
      (experimental group) for 7 days. The morphological changes of the cells were 
      recorded; the protein levels of cytokeratin 18 (CK18), alpha fetoprotein (AFP), 
      and CYP3A4 were measured using Western blot. The glycogen storing ability of the 
      cells was detected by periodic acid-schiff (PAS) staining. Furthermore, the 
      synthesis of albumin (ALB) and blood urea nitrogen (BUN) was measured. RESULTS: 
      In experimental group, after 1 day of induction, the stem cells of fusiform shape 
      began to lose sharp edges and progressively shrunk, and then they changed into 
      hepatocyte-like cells with round and irregular shape at 7 days. Positive 
      expressions of AFP, CK18, and CYP3A4 were observed in the experimental group, but 
      negative expression in the control group. The concentrations of BUN and ALB were 
      (0.43 +/- 0.07) mmol/L and (8.08 +/- 0.41) microg/mL in the control group and were (2.52 
      +/- 0.20) mmol/L and (41.48 +/- 4.11) microg/mL in the experimental group, showing 
      significant differences (t=24.160, P = 0.000; t = 19.810, P = 0.000). PAS 
      staining results showed navy blue nucleus and lavender cytoplasm in the control 
      group, but dark purple cell body and visible nucleus in the experimental group. 
      CONCLUSION: HUCMSCs could differentiate into hepatocyte-like cells induced by rat 
      fibrotic liver tissue extracts, which have hepatocyte biomarkers (AFP, CK18, and 
      CYP3A4) and hepatocyte-specific functions of glycogen storage, urea production 
      and ALB secretion, so they could partially replace the function of hepatocytes, 
      that may be one of the therapeutic mechanisms of stem cell transplantation.
FAU - Yan, Cheng
AU  - Yan C
FAU - Xue, Gai
AU  - Xue G
FAU - Wu, Liying
AU  - Wu L
FAU - Liu, Jianfang
AU  - Liu J
FAU - Hou, Yanning
AU  - Hou Y
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Keratin-18)
RN  - 0 (Liver Extracts)
RN  - 0 (Tissue Extracts)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*drug effects/physiology
MH  - Cells, Cultured
MH  - Cord Blood Stem Cell Transplantation
MH  - Fetal Blood/*cytology
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Hepatocytes/*cytology/drug effects/metabolism
MH  - Humans
MH  - Keratin-18/biosynthesis
MH  - Liver/embryology
MH  - Liver Extracts/*pharmacology
MH  - Mesenchymal Stem Cells/*cytology/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cells/cytology/drug effects
MH  - Tissue Extracts
EDAT- 2015/11/07 06:00
MHDA- 2016/07/13 06:00
CRDT- 2015/11/07 06:00
PHST- 2015/11/07 06:00 [entrez]
PHST- 2015/11/07 06:00 [pubmed]
PHST- 2016/07/13 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2015 Jul;29(7):878-83.