PMID- 26541434
OWN - NLM
STAT- MEDLINE
DCOM- 20160708
LR  - 20220311
IS  - 1468-2079 (Electronic)
IS  - 0007-1161 (Linking)
VI  - 100
IP  - 3
DP  - 2016 Mar
TI  - MicroRNAs in retina during development of experimental autoimmune uveoretinitis 
      in rats.
PG  - 425-31
LID - 10.1136/bjophthalmol-2015-306924 [doi]
AB  - PURPOSE: To determine the changes in the expression profiles of microRNAs 
      (miRNAs) in retinas during the development of experimental autoimmune 
      uveoretinitis (EAU) in rats. METHODS: The levels of interleukin-1beta (IL-1beta) and 
      monocyte chemoattractant protein-1 (MCP-1) were measured in aqueous humour 
      samples and supernatants of homogenised posterior eye cups obtained from Lewis 
      rats immunised with interphotoreceptor retinoid binding protein peptide (R14) and 
      complete Freund's adjuvant. Microarray analysis was performed to determine the 
      miRNA profiles in the retina of eyes with EAU on days 0 (baseline), 7, 14 and 21 
      after immunisation. RESULTS: The levels of IL-1beta and MCP-1 in the aqueous humour 
      and the supernatants of posterior eye cups were significantly elevated in eyes 
      with EAU, and the levels corresponded with the stage of the EAU. On day 14 after 
      immunisation, the expressions of nine miRNAs (miRNA-223, 142-5p, 142-3p, 21, 
      146a, 146b, 1949, 1188-3p and 193) were significantly elevated, and the 
      expressions of four miRNAs (miRNA-181a, 183*, 124* and 331) were downregulated 
      relative to the baseline. Quantitative PCR analyses confirmed the elevation of 
      miRNA-223 and miRNA-146 and the downregulation of miRNA-181a in retinas with EAU 
      on day 14 after immunisation. In situ hybridisation confirmed increased 
      expression of miR-223 and miR-146 in retinas with EAU. CONCLUSIONS: Several 
      miRNAs were significantly increased or decreased in retinas during the course of 
      EAU. The expression of miR-223 and miR-146a corresponded with the clinical score 
      of the EAU and elevation of IL-1beta/MCP-1 in the eye with EAU. Further studies are 
      required to clarify the role of miRNA in eyes with autoimmune uveoretinitis.
CI  - Published by the BMJ Publishing Group Limited. For permission to use (where not 
      already granted under a licence) please go to 
      http://www.bmj.com/company/products-services/rights-and-licensing/
FAU - Watanabe, Takayo
AU  - Watanabe T
AD  - Department of Ophthalmology, Kyorin University School of Medicine, Tokyo, Japan.
FAU - Keino, Hiroshi
AU  - Keino H
AD  - Department of Ophthalmology, Kyorin University School of Medicine, Tokyo, Japan.
FAU - Kudo, Akihiko
AU  - Kudo A
AD  - Department of Anatomy, Kyorin University School of Medicine, Tokyo, Japan.
FAU - Sato, Yasuhiko
AU  - Sato Y
AD  - Division of Radioisotope Research, Kyorin University School of Medicine, Tokyo, 
      Japan.
FAU - Okada, Annabelle A
AU  - Okada AA
AD  - Department of Ophthalmology, Kyorin University School of Medicine, Tokyo, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151105
PL  - England
TA  - Br J Ophthalmol
JT  - The British journal of ophthalmology
JID - 0421041
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1beta)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Aqueous Humor/metabolism
MH  - Autoimmune Diseases/*genetics/metabolism/pathology
MH  - Chemokine CCL2/metabolism
MH  - *Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*physiology
MH  - In Situ Hybridization
MH  - Interleukin-1beta/metabolism
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Oligonucleotide Array Sequence Analysis
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Real-Time Polymerase Chain Reaction
MH  - Retinitis/*genetics/metabolism/pathology
MH  - Uveitis/*genetics/metabolism/pathology
OTO - NOTNLM
OT  - Immunology
OT  - Inflammation
EDAT- 2015/11/07 06:00
MHDA- 2016/07/09 06:00
CRDT- 2015/11/07 06:00
PHST- 2015/03/27 00:00 [received]
PHST- 2015/10/16 00:00 [accepted]
PHST- 2015/11/07 06:00 [entrez]
PHST- 2015/11/07 06:00 [pubmed]
PHST- 2016/07/09 06:00 [medline]
AID - bjophthalmol-2015-306924 [pii]
AID - 10.1136/bjophthalmol-2015-306924 [doi]
PST - ppublish
SO  - Br J Ophthalmol. 2016 Mar;100(3):425-31. doi: 10.1136/bjophthalmol-2015-306924. 
      Epub 2015 Nov 5.