PMID- 26541633
OWN - NLM
STAT- MEDLINE
DCOM- 20160929
LR  - 20151125
IS  - 1096-0937 (Electronic)
IS  - 1087-1845 (Linking)
VI  - 85
DP  - 2015 Dec
TI  - Role of BGT-1 and BGT-2, two predicted GPI-anchored glycoside 
      hydrolases/glycosyltransferases, in cell wall remodeling in Neurospora crassa.
PG  - 58-70
LID - S1087-1845(15)30042-6 [pii]
LID - 10.1016/j.fgb.2015.11.001 [doi]
AB  - Neurospora crassa BGT-1 (NCU06381) and BGT-2 (NCU09175) are two putative 
      glycoside hydrolases (GHs) with additional predicted glycosyltransferase activity 
      and binding sites for a glycosyl phosphatidyl inositol (GPI) anchor that would 
      facilitate their attachment to the plasma membrane (PM). To discern their role in 
      key morphogenetic events during vegetative development of N. crassa, BGT-1 and 
      BGT-2 were labeled with the green fluorescent protein (GFP). The gfp was inserted 
      immediately after the signal peptide sequence, within the bgt-1 encoding 
      sequence, or directly before the GPI-binding site in the case of bgt-2. Both 
      BGT-1-GFP and BGT-2-GFP were observed at the PM of the hyphal apical dome, 
      excluding the foremost apical region and the Spitzenkorper (Spk), where chitin 
      and beta-1,3-glucan synthases have been previously found. These and previous studies 
      suggest a division of labor of the cell wall synthesizing machinery at the hyphal 
      dome: at the very tip, glucans are synthesized by enzymes that accumulate at the 
      Spk, before getting incorporated into the PM, whereas at the subtending zone 
      below the apex, glucans are presumably hydrolyzed, producing amenable ends for 
      further branching and crosslinking with other cell wall polymers. Additionally, 
      BGT-1-GFP and BGT-2-GFP were observed at the leading edge of new developing 
      septa, at unreleased interconidial junctions, at conidial poles, at germling and 
      hyphal fusion sites, and at sites of branch emergence, all of them processes that 
      seemingly involve cell wall remodeling. Even though single and double mutant 
      strains for the corresponding genes did not show a drastic reduction of growth 
      rate, bgt-2Delta and bgt-1Delta::bgt-2Delta strains exhibited an increased resistance to the 
      cell wall stressors calcofluor white (CW) and congo red (CR) than the reference 
      strain, which suggests they present significant architectural changes in their 
      cell wall. Furthermore, the conidiation defects observed in the mutants indicate 
      a significant role of BGT-1 and BGT-2 on the re-arrangement of glucans needed at 
      the conidiophore cell wall to allow conidial separation.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Martinez-Nunez, Leonora
AU  - Martinez-Nunez L
AD  - Department of Microbiology, Centro de Investigacion Cientifica y de Educacion 
      Superior de Ensenada-CICESE, Ensenada, Baja California 22860, Mexico.
FAU - Riquelme, Meritxell
AU  - Riquelme M
AD  - Department of Microbiology, Centro de Investigacion Cientifica y de Educacion 
      Superior de Ensenada-CICESE, Ensenada, Baja California 22860, Mexico. Electronic 
      address: riquelme@cicese.mx.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151102
PL  - United States
TA  - Fungal Genet Biol
JT  - Fungal genetics and biology : FG & B
JID - 9607601
RN  - 0 (Fungal Proteins)
RN  - 0 (Glycosylphosphatidylinositols)
RN  - 0 (beta-Glucans)
RN  - 1398-61-4 (Chitin)
RN  - 9051-97-2 (beta-1,3-glucan)
RN  - EC 2.4.- (Glycosyltransferases)
RN  - EC 3.2.1.- (Glycoside Hydrolases)
SB  - IM
MH  - Cell Membrane/metabolism
MH  - Cell Wall/*metabolism
MH  - Chitin/metabolism
MH  - Fungal Proteins/genetics/metabolism
MH  - Genes, Reporter
MH  - Glycoside Hydrolases/genetics/*metabolism
MH  - Glycosylphosphatidylinositols/metabolism
MH  - Glycosyltransferases/genetics/*metabolism
MH  - Hyphae
MH  - Neurospora crassa/cytology/*enzymology/genetics/growth & development
MH  - Spores, Fungal
MH  - beta-Glucans/metabolism
OTO - NOTNLM
OT  - Cell wall remodeling
OT  - GFP tagging
OT  - Neurospora crassa
OT  - beta-1,3-Endoglucanases
EDAT- 2015/11/07 06:00
MHDA- 2016/09/30 06:00
CRDT- 2015/11/07 06:00
PHST- 2015/08/06 00:00 [received]
PHST- 2015/10/25 00:00 [revised]
PHST- 2015/11/01 00:00 [accepted]
PHST- 2015/11/07 06:00 [entrez]
PHST- 2015/11/07 06:00 [pubmed]
PHST- 2016/09/30 06:00 [medline]
AID - S1087-1845(15)30042-6 [pii]
AID - 10.1016/j.fgb.2015.11.001 [doi]
PST - ppublish
SO  - Fungal Genet Biol. 2015 Dec;85:58-70. doi: 10.1016/j.fgb.2015.11.001. Epub 2015 
      Nov 2.