PMID- 26543079
OWN - NLM
STAT- MEDLINE
DCOM- 20160912
LR  - 20151106
IS  - 1790-6245 (Electronic)
IS  - 1109-6535 (Linking)
VI  - 12
IP  - 6
DP  - 2015 Nov-Dec
TI  - Oligo-based High-resolution aCGH Analysis Enhances Routine Cytogenetic 
      Diagnostics in Haematological Malignancies.
PG  - 301-37
AB  - BACKGROUND: The purpose of the present study was to evaluate the detection rate 
      of genomic aberrations in haematological malignancies using oligobased array-CGH 
      (oaCGH) analysis in combination with karyotyping and fluorescence in situ 
      hybridization (FISH) analyses, and its feasibility in a clinical pragmatic 
      approach. MATERIALS AND METHODS: The 4x180K Cancer Cytochip array was applied in 
      96 patients with various haematological malignancies in a prospective setting and 
      in 41 acute myeloid leukemia (AML) patients retrospectively. RESULTS: Combined 
      use of oaCGH analysis and karyotyping improved the overall detection rate in 
      comparison to karyotyping-alone and vice versa. In cases with normal karyotypes 
      oaCGH analysis detected genomic aberrations in 66% (39/60) of cases. In the group 
      of simple karyotypes oaCGH analysis extended karyotypic findings in 39% (12/31) 
      while oaCGH analysis extended the karyotypic findings in 89% (39/44) of cases 
      with complex karyotypes. In 7% (5/75) of cases oaCGH analysis failed in detecting 
      the observed abnormalities by karyotyping. CONCLUSION: oaCGH analysis is a 
      valuable asset in routine cytogenetics of haematological malignancies.
CI  - Copyright(c) 2015, International Institute of Anticancer Research (Dr. John G. 
      Delinasios), All rights reserved.
FAU - Kjeldsen, Eigil
AU  - Kjeldsen E
AD  - HaemoDiagnostic Laboratory, Cancer Cytogenetics Section, Department of 
      Hematology, Aarhus University Hospital, Aarhus, Denmark 
      Eigil.Kjeldsen@clin.au.dk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Cancer Genomics Proteomics
JT  - Cancer genomics & proteomics
JID - 101188791
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - Chromosome Banding
MH  - *Comparative Genomic Hybridization
MH  - Cytogenetics/*methods
MH  - Female
MH  - Gene Rearrangement
MH  - Hematologic Neoplasms/*diagnosis/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/*diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Oligonucleotide Array Sequence Analysis
MH  - Prospective Studies
MH  - Retrospective Studies
OTO - NOTNLM
OT  - FISH
OT  - Haematological malignancy
OT  - aCGH
OT  - chromothripsis
OT  - karyotyping
EDAT- 2015/11/07 06:00
MHDA- 2016/09/13 06:00
CRDT- 2015/11/07 06:00
PHST- 2015/11/07 06:00 [entrez]
PHST- 2015/11/07 06:00 [pubmed]
PHST- 2016/09/13 06:00 [medline]
AID - 12/6/301 [pii]
PST - ppublish
SO  - Cancer Genomics Proteomics. 2015 Nov-Dec;12(6):301-37.