PMID- 26543792
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151106
LR  - 20200930
IS  - 2193-1801 (Print)
IS  - 2193-1801 (Electronic)
IS  - 2193-1801 (Linking)
VI  - 4
DP  - 2015
TI  - Hydrolyzed fish proteins reduced activation of caspase-3 in H2O2 induced 
      oxidative stressed liver cells isolated from Atlantic salmon (Salmo salar).
PG  - 658
LID - 10.1186/s40064-015-1432-6 [doi]
LID - 658
AB  - Hydrolyzed fish proteins (H-pro) contains high concentrations of free amino acids 
      and low molecular peptides that potentially benefit health. The following study 
      aimed to test whether the water soluble phase of H-pro could reduce apoptosis and 
      inflammation in primary liver cells isolated from Atlantic salmon following H2O2 
      provoked oxidative stress. Cells were grown as monocultures or co-cultured with 
      head kidney cells to assess possible cross talk in inflammation and metabolism 
      during treatments. Cells were grown in media with or without H-pro for 2 days 
      before being stressed with 200 microM H2O2 then harvested 24 h post exposure. Both 
      treatments were compared to the respective treatments without H2O2 
      supplementation. Oxidative stressed cells had increased activation of caspase-3, 
      but supplementation with H-pro in the media prior to the oxidative stress reduced 
      caspase-3 activation. In conclusion, free amino acids and low molecular weight 
      peptides from H-pro attenuated oxidative stress, and made cells able to withstand 
      apoptosis after H2O2 provoked oxidative stress.
FAU - Espe, M
AU  - Espe M
AD  - National Institute of Nutrition and Seafood Research (NIFES), PoBox 2029, 5817 
      Nordnes, Norway.
FAU - Holen, E
AU  - Holen E
AD  - National Institute of Nutrition and Seafood Research (NIFES), PoBox 2029, 5817 
      Nordnes, Norway.
FAU - He, J
AU  - He J
AD  - National Institute of Nutrition and Seafood Research (NIFES), PoBox 2029, 5817 
      Nordnes, Norway ; Fish Nutrition Laboratory, Institute of Aquatic Economic 
      Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
FAU - Provan, F
AU  - Provan F
AD  - International Research Institute of Stavanger AS (IRIS), PoBox 8046, 4068 
      Stavanger, Norway.
FAU - Chen, L
AU  - Chen L
AD  - National Institute of Nutrition and Seafood Research (NIFES), PoBox 2029, 5817 
      Nordnes, Norway ; School of Life Science, East China Normal University, Shanghai, 
      China.
FAU - Oysaed, K B
AU  - Oysaed KB
AD  - International Research Institute of Stavanger AS (IRIS), PoBox 8046, 4068 
      Stavanger, Norway.
FAU - Seliussen, J
AU  - Seliussen J
AD  - Hordafor AS, Salthella, 5397 Bekkjarvik, Norway.
LA  - eng
PT  - Journal Article
DEP - 20151031
PL  - Switzerland
TA  - Springerplus
JT  - SpringerPlus
JID - 101597967
PMC - PMC4628607
OTO - NOTNLM
OT  - Atlantic salmon
OT  - Caspase-3
OT  - Co-cultures
OT  - Head kidney cells
OT  - Hydrolyzed proteins
OT  - Oxidative stress
OT  - Primary liver cells
OT  - Viability
EDAT- 2015/11/07 06:00
MHDA- 2015/11/07 06:01
PMCR- 2015/10/31
CRDT- 2015/11/07 06:00
PHST- 2015/08/25 00:00 [received]
PHST- 2015/10/12 00:00 [accepted]
PHST- 2015/11/07 06:00 [entrez]
PHST- 2015/11/07 06:00 [pubmed]
PHST- 2015/11/07 06:01 [medline]
PHST- 2015/10/31 00:00 [pmc-release]
AID - 1432 [pii]
AID - 10.1186/s40064-015-1432-6 [doi]
PST - epublish
SO  - Springerplus. 2015 Oct 31;4:658. doi: 10.1186/s40064-015-1432-6. eCollection 
      2015.