PMID- 26543853
OWN - NLM
STAT- MEDLINE
DCOM- 20160927
LR  - 20181113
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2015
DP  - 2015
TI  - Decreased Splenic CD4(+) T-Lymphocytes in Apolipoprotein M Gene Deficient Mice.
PG  - 293512
LID - 10.1155/2015/293512 [doi]
LID - 293512
AB  - Spleen T-lymphocytes, especially CD4(+) T-cells, have been demonstrated to be 
      involved in broad immunomodulation and host-defense activity in vivo. 
      Apolipoprotein M gene (apoM) may have an important role in the regulation of 
      immunoprocess and inflammation, which could be hypothesized to the apoM 
      containing sphingosine-1-phosphate (S1P). In the present study we demonstrate 
      that the splenic CD4(+) T-lymphocytes were obviously decreased in the apoM gene 
      deficient (apoM(-/-)) mice compared to the wild type (apoM(+/+)). Moreover, these 
      mice were treated with lipopolysaccharide (LPS) and it was found that even more 
      pronounced decreasing CD4(+) T-lymphocytes occurred in the spleen compared to the 
      apoM(+/+) mice. The similar phenomena were found in the ratio of CD4(+)/CD8(+) 
      T-lymphocytes. After administration of LPS, the hepatic mRNA levels of tumor 
      necrosis factor-alpha (TNF-alpha) and monocyte chemotactic protein-1 (MCP-1) were 
      markedly increased; however, there were no statistical differences observed 
      between apoM(+/+) mice and apoM(-/-) mice. The present study demonstrated that 
      apoM might facilitate the maintenance of CD4(+) T-lymphocytes or could modify the 
      T-lymphocytes subgroups in murine spleen, which may further explore the 
      importance of apoM in the regulation of the host immunomodulation, although the 
      detailed mechanism needs continuing investigation.
FAU - Wang, Zhigang
AU  - Wang Z
AD  - Department of Cardiothoracic Surgery, Third Affiliated Hospital of Soochow 
      University, Changzhou 213003, China.
FAU - Luo, Guanghua
AU  - Luo G
AD  - Comprehensive Laboratory, Third Affiliated Hospital of Soochow University, 
      Changzhou 213003, China.
FAU - Feng, Yuehua
AU  - Feng Y
AD  - Comprehensive Laboratory, Third Affiliated Hospital of Soochow University, 
      Changzhou 213003, China.
FAU - Zheng, Lu
AU  - Zheng L
AD  - Comprehensive Laboratory, Third Affiliated Hospital of Soochow University, 
      Changzhou 213003, China.
FAU - Liu, Hongyao
AU  - Liu H
AD  - Department of Cardiothoracic Surgery, Third Affiliated Hospital of Soochow 
      University, Changzhou 213003, China.
FAU - Liang, Yun
AU  - Liang Y
AD  - Department of Cardiothoracic Surgery, Third Affiliated Hospital of Soochow 
      University, Changzhou 213003, China.
FAU - Liu, Zhonghua
AU  - Liu Z
AD  - Department of Cardiothoracic Surgery, Third Affiliated Hospital of Soochow 
      University, Changzhou 213003, China.
FAU - Shao, Peng
AU  - Shao P
AD  - Department of Cardiothoracic Surgery, Third Affiliated Hospital of Soochow 
      University, Changzhou 213003, China.
FAU - Berggren-Soderlund, Maria
AU  - Berggren-Soderlund M
AD  - Division of Clinical Chemistry and Pharmacology, Department of Laboratory 
      Medicine, Lund University, 221 85 Lund, Sweden.
FAU - Zhang, Xiaoying
AU  - Zhang X
AD  - Department of Cardiothoracic Surgery, Third Affiliated Hospital of Soochow 
      University, Changzhou 213003, China.
FAU - Xu, Ning
AU  - Xu N
AD  - Division of Clinical Chemistry and Pharmacology, Department of Laboratory 
      Medicine, Lund University, 221 85 Lund, Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151012
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (ApoM protein, mouse)
RN  - 0 (Apolipoproteins)
RN  - 0 (Apolipoproteins M)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Lysophospholipids)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 26993-30-6 (sphingosine 1-phosphate)
RN  - NGZ37HRE42 (Sphingosine)
SB  - IM
MH  - Adaptive Immunity
MH  - Animals
MH  - Apolipoproteins/*genetics
MH  - Apolipoproteins M
MH  - CD4-Positive T-Lymphocytes/*cytology
MH  - CD8-Positive T-Lymphocytes/cytology
MH  - Chemokine CCL2/genetics
MH  - Cytokines/metabolism
MH  - Flow Cytometry
MH  - Inflammation
MH  - Lipopolysaccharides/metabolism
MH  - Liver/metabolism
MH  - Lysophospholipids/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Real-Time Polymerase Chain Reaction
MH  - Sphingosine/analogs & derivatives/metabolism
MH  - Spleen/*cytology/immunology
MH  - Tumor Necrosis Factor-alpha/genetics
PMC - PMC4620415
EDAT- 2015/11/07 06:00
MHDA- 2016/09/28 06:00
PMCR- 2015/10/12
CRDT- 2015/11/07 06:00
PHST- 2015/07/16 00:00 [received]
PHST- 2015/09/23 00:00 [revised]
PHST- 2015/09/28 00:00 [accepted]
PHST- 2015/11/07 06:00 [entrez]
PHST- 2015/11/07 06:00 [pubmed]
PHST- 2016/09/28 06:00 [medline]
PHST- 2015/10/12 00:00 [pmc-release]
AID - 10.1155/2015/293512 [doi]
PST - ppublish
SO  - Biomed Res Int. 2015;2015:293512. doi: 10.1155/2015/293512. Epub 2015 Oct 12.