PMID- 26544637
OWN - NLM
STAT- MEDLINE
DCOM- 20170417
LR  - 20221207
IS  - 1827-1839 (Electronic)
IS  - 0392-9590 (Linking)
VI  - 35
IP  - 4
DP  - 2016 Aug
TI  - Association of TNF-alpha gene variations with thoracic aortic dissection risk in a 
      Chinese Han population.
PG  - 418-24
AB  - BACKGROUND: Chronic inflammation may be involved in pathogenesis of thoracic 
      aortic dissection (TAD). Tumor necrosis factor-alpha (TNF-alpha) is a proinflammatory 
      cytokine that plays an important role in pathological TAD progression. In this 
      study, we determined wether genetic variants of TNF-alpha were associated with TAD. 
      METHODS: Frequency distributions of TNF-alpha promoter polymorphisms 
      (-1031C/T,-857C/T,-308G/A, and -238G/A) were determined by direct sequencing. 
      TNF-alpha plasma levels were measured by enzyme-linked immunosorbent assay. Plasma 
      levels of TNF-alpha mRNA in peripheral-blood mononuclear cells were analyzed by 
      real-time quantitative polymerase chain reaction amplification. RESULTS: We found 
      the TNF-alpha promoter -857C/T polymorphism is associated with disease progression 
      susceptibility in TAD patients. The CC homozygote of TAD patients had a 
      significantly higher risk of TAD than did T allele carriers (P< 0.05). Plasma 
      TNF-alpha concentrations were also significantly higher in TAD patients than control 
      subjects (P<0.05), and CC genotype carriers showed increased TNF-alpha levels 
      compared with T allele carriers (P<0.05). Moreover, peripheral-blood mononuclear 
      cells carrying the CC genotype showed increased TNF-alpha mRNA levels compared with 
      cells carrying the T allele. CONCLUSIONS: The -857C/T polymorphism of TNF-alpha 
      promoter plays a role in the genetic variation underlying susceptibility of 
      individuals to TAD progression. The CC genotype is associated with increased 
      TNF-alpha expression in TAD patients, and may be an independent predictive factor for 
      TAD.
FAU - DU, Xiao M
AU  - DU XM
AD  - Department of Cardiology, Cardiovascular Research Institute, the General Hospital 
      of Shenyang Military, Shenyang, Liaoning, China - wxiaozeng@163.com.
FAU - Liu, Li W
AU  - Liu LW
FAU - DU, Zhan K
AU  - DU ZK
FAU - Gu, Ruo X
AU  - Gu RX
FAU - Han, Ya L
AU  - Han YL
FAU - Wang, Xiao Z
AU  - Wang XZ
LA  - eng
PT  - Journal Article
DEP - 20151106
PL  - Italy
TA  - Int Angiol
JT  - International angiology : a journal of the International Union of Angiology
JID - 8402693
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Aortic Dissection/diagnosis/ethnology/*genetics
MH  - Aortic Aneurysm, Thoracic/blood/diagnosis/ethnology/*genetics
MH  - Asian People/genetics
MH  - Case-Control Studies
MH  - China/epidemiology
MH  - Disease Progression
MH  - Female
MH  - Gene Frequency
MH  - Genetic Association Studies
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Phenotype
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/blood/genetics
MH  - Risk Factors
MH  - Tumor Necrosis Factor-alpha/blood/*genetics
EDAT- 2015/11/07 06:00
MHDA- 2017/04/18 06:00
CRDT- 2015/11/07 06:00
PHST- 2015/11/07 06:00 [entrez]
PHST- 2015/11/07 06:00 [pubmed]
PHST- 2017/04/18 06:00 [medline]
AID - R34Y9999N00A150071 [pii]
PST - ppublish
SO  - Int Angiol. 2016 Aug;35(4):418-24. Epub 2015 Nov 6.