PMID- 26546328
OWN - NLM
STAT- MEDLINE
DCOM- 20160929
LR  - 20151128
IS  - 2049-632X (Electronic)
IS  - 2049-632X (Linking)
VI  - 74
IP  - 1
DP  - 2016 Feb
TI  - Persistence of non-typeable Haemophilus Influenzae in the pharynx of children 
      with adenotonsillar hypertrophy after treatment with azithromycin.
PG  - ftv106
LID - ftv106 [pii]
LID - 10.1093/femspd/ftv106 [doi]
AB  - This study was performed in children with adenotonsillar hypertrophy to evaluate 
      the effect of azithromycin (AZT) on the presence of NTHi in monocyte/macrophages 
      (CD14(+) cells) of adenoids/tonsils and the persistence of NTHi after 
      adenotonsillectomy. A total of 36 pediatric patients participated in the study: 
      20 children were treated with AZT before adenotonsillectomy, and 16 children did 
      not receive the antibiotic prior to surgery. NTHi were identified by culture and 
      PCR in swabs and tissue samples. NTHi was detected in the lysates of CD14(+) 
      cells by fluorescence in situ hybridization (FISH) and by culture. The molecular 
      typing was used to cluster NTHi isolates from each child. The intracellular NTHi 
      was found in 10 (62.5%) untreated patients and was identified in three (15%) 
      azithromycin-treated patients (P = 0.003). The proportion of the persistent NTHi 
      strains was similar in both groups. AZT treatment followed by adenotonsillectomy 
      did not completely eliminate NTHi from pharynges; however, it significantly 
      reduced the risk of carriage of Haemophilus influenzae inside the CD14(+) cells.
CI  - (c) FEMS 2015. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Olszewska-Sosinska, O
AU  - Olszewska-Sosinska O
AD  - Department of Otolaryngology Children's Hospital, Nieklanska 4/24, 03-924 Warsaw, 
      Poland.
FAU - Zielnik-Jurkiewicz, B
AU  - Zielnik-Jurkiewicz B
AD  - Department of Otolaryngology Children's Hospital, Nieklanska 4/24, 03-924 Warsaw, 
      Poland.
FAU - Stepinska, M
AU  - Stepinska M
AD  - Department of Microbiology, Military Institute of Hygiene and Epidemiology, 
      Kozielska 4, 01-163 Warsaw, Poland.
FAU - Antos-Bielska, M
AU  - Antos-Bielska M
AD  - Department of Microbiology, Military Institute of Hygiene and Epidemiology, 
      Kozielska 4, 01-163 Warsaw, Poland.
FAU - Lau-Dworak, M
AU  - Lau-Dworak M
AD  - Department of Laboratory Diagnostics, Children's Hospital, Nieklanska 4/24, 
      03-924 Warsaw, Poland.
FAU - Kozlowska, K
AU  - Kozlowska K
AD  - Department of Microbiology, Military Institute of Hygiene and Epidemiology, 
      Kozielska 4, 01-163 Warsaw, Poland.
FAU - Trafny, E A
AU  - Trafny EA
AD  - Department of Microbiology, Military Institute of Hygiene and Epidemiology, 
      Kozielska 4, 01-163 Warsaw, Poland trafny.elzbieta@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151106
PL  - United States
TA  - Pathog Dis
JT  - Pathogens and disease
JID - 101595366
RN  - 0 (Anti-Bacterial Agents)
RN  - 83905-01-5 (Azithromycin)
SB  - IM
MH  - Anti-Bacterial Agents/*therapeutic use
MH  - Azithromycin/*therapeutic use
MH  - Carrier State/drug therapy/*microbiology
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Haemophilus Infections/drug therapy/*microbiology
MH  - Haemophilus influenzae/classification/*isolation & purification
MH  - Humans
MH  - Hypertrophy/*pathology
MH  - Lymph Nodes/pathology
MH  - Male
MH  - Molecular Typing
MH  - Palatine Tonsil/pathology
MH  - Pharynx/*microbiology
MH  - Prospective Studies
OTO - NOTNLM
OT  - NTHi
OT  - adenoid
OT  - azithromycin
OT  - children
OT  - macrophages
EDAT- 2015/11/08 06:00
MHDA- 2016/09/30 06:00
CRDT- 2015/11/08 06:00
PHST- 2015/10/30 00:00 [accepted]
PHST- 2015/11/08 06:00 [entrez]
PHST- 2015/11/08 06:00 [pubmed]
PHST- 2016/09/30 06:00 [medline]
AID - ftv106 [pii]
AID - 10.1093/femspd/ftv106 [doi]
PST - ppublish
SO  - Pathog Dis. 2016 Feb;74(1):ftv106. doi: 10.1093/femspd/ftv106. Epub 2015 Nov 6.