PMID- 26546748
OWN - NLM
STAT- MEDLINE
DCOM- 20161005
LR  - 20161230
IS  - 1872-7077 (Electronic)
IS  - 1382-6689 (Linking)
VI  - 40
IP  - 3
DP  - 2015 Nov
TI  - p-Methoxycinnamic acid, an active phenylpropanoid induces mitochondrial mediated 
      apoptosis in HCT-116 human colon adenocarcinoma cell line.
PG  - 966-74
LID - S1382-6689(15)30091-0 [pii]
LID - 10.1016/j.etap.2015.09.013 [doi]
AB  - Among the eight phytochemicals (dihydrocarveol, sinapic acid, vanillic acid, 
      ethylgallate, myrtenol, transcarveol, p-methoxycinnamic acid, and isoferulic 
      acid) we tested, p-methoxycinnamic acid (p-MCA) [10 muM] showed the most potent in 
      vitro growth inhibition on human colon adenocarcinoma (HCT-116 cells). 
      Antiproliferative activity of p-MCA at 24h was associated with DNA damage, 
      morphological changes and the results were comparable with doxorubicin. p-MCA 
      induced phosphatidylserine translocation, increased the levels of reactive oxygen 
      species (ROS), thiobarbituric acid reactive substances (TBARS), protein carbonyl 
      content (PCC) and decreased enzymic antioxidant status (SOD, CAT, GPx) in 
      HCT-116. p-MCA treatment increased the percentage of apoptotic cells, decreased 
      the mitochondrial membrane potential and triggered cytochrome C release to 
      cytosol. The induction of apoptosis by p-MCA was accompanied by an increase in 
      caspase 3 and caspase 9 activities, increased expression of Bax and decreased 
      expression of Bcl-2. Thus p-MCA induces mitochondria mediated intrinsic pathway 
      of apoptosis in HCT-116 and has potential for treatment and prevention of colon 
      cancer.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Gunasekaran, Sivagami
AU  - Gunasekaran S
AD  - Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai 
      University, Annamalai Nagar, Tamil Nadu 608 002, India.
FAU - Venkatachalam, Karthikkumar
AU  - Venkatachalam K
AD  - Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai 
      University, Annamalai Nagar, Tamil Nadu 608 002, India.
FAU - Namasivayam, Nalini
AU  - Namasivayam N
AD  - Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai 
      University, Annamalai Nagar, Tamil Nadu 608 002, India. Electronic address: 
      nalininam@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20150926
PL  - Netherlands
TA  - Environ Toxicol Pharmacol
JT  - Environmental toxicology and pharmacology
JID - 9612020
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Cinnamates)
RN  - 0 (Phytochemicals)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Adenocarcinoma/*metabolism
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cinnamates/*pharmacology
MH  - Colonic Neoplasms/*metabolism
MH  - HCT116 Cells
MH  - HT29 Cells
MH  - Humans
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Mitochondria/*drug effects
MH  - Oxidative Stress/drug effects
MH  - Phytochemicals/pharmacology
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - Caspase 3
OT  - Caspase 9
OT  - Cell viability
OT  - Mitochondrial membrane potential
OT  - Phosphatidylserine translocation
OT  - p-Methoxycinnamic acid
EDAT- 2015/11/08 06:00
MHDA- 2016/10/07 06:00
CRDT- 2015/11/08 06:00
PHST- 2015/06/16 00:00 [received]
PHST- 2015/09/22 00:00 [accepted]
PHST- 2015/11/08 06:00 [entrez]
PHST- 2015/11/08 06:00 [pubmed]
PHST- 2016/10/07 06:00 [medline]
AID - S1382-6689(15)30091-0 [pii]
AID - 10.1016/j.etap.2015.09.013 [doi]
PST - ppublish
SO  - Environ Toxicol Pharmacol. 2015 Nov;40(3):966-74. doi: 
      10.1016/j.etap.2015.09.013. Epub 2015 Sep 26.