PMID- 26547433
OWN - NLM
STAT- MEDLINE
DCOM- 20160912
LR  - 20151207
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 77
DP  - 2016 Mar 15
TI  - Functionalized gold nanoclusters as fluorescent labels for immunoassays: 
      Application to human serum immunoglobulin E determination.
PG  - 1055-61
LID - S0956-5663(15)30336-5 [pii]
LID - 10.1016/j.bios.2015.08.011 [doi]
AB  - A quantitative immunoassay for the determination of immunoglobulin E (IgE) in 
      human serum using gold nanoclusters (AuNCs) as fluorescent label was developed. 
      Water soluble AuNCs were synthesized using lipoic acid and then thoroughly 
      characterized. The obtained AuNCs have a particle size of 2.7 +/- 0.1 nm and 
      maximum fluorescence emission at 710 nm. The synthesized AuNCs showed very good 
      stability of the fluorescent signal with light exposure and at neutral and 
      slightly basic media. A covalent bioconjugation of these AuNCs with the desired 
      antibody was carried out by the carbodiimide reaction. After due optimization of 
      such bioconjugation reaction, a molar ratio 1:3 (antibody:AuNCs) was selected. 
      The bioconjugate maintained an intense luminescence emission, slightly 
      red-shifted as compared to the free AuNCs. Two typical immunoassay 
      configurations, competitive and sandwich, were assayed and their performance for 
      IgE determination critically compared. After the different immunoassay steps were 
      accomplished, the fluorescence emission of the bioconjugate was measured. While 
      the sandwich format provided a detection limit (DL) of 10 ng/mL and a linear 
      range between 25 and 565 ng/mL of IgE, the competitive format revealed a DL of 
      0.2 ng/mL with a linear range between 0.3 and 7.1 ng/mL The applicability of the 
      more sensitive competitive fluorescent immunoassay was assessed by successful 
      analysis of the IgE in human serum and comparison of results with those from a 
      commercial kit. The main advantages of the proposed AuNCs-based fluorimetric 
      method include a low DL and a simple immunoassay protocol involving few reagents.
CI  - Copyright (c) 2015. Published by Elsevier B.V.
FAU - Alonso, Maria Cruz
AU  - Alonso MC
AD  - Department of Physical and Analytical Chemistry, Faculty of Chemistry, University 
      of Oviedo, Julian Claveria, 8, 33006 Oviedo, Spain.
FAU - Trapiella-Alfonso, Laura
AU  - Trapiella-Alfonso L
AD  - Department of Physical and Analytical Chemistry, Faculty of Chemistry, University 
      of Oviedo, Julian Claveria, 8, 33006 Oviedo, Spain.
FAU - Fernandez, Jose M Costa
AU  - Fernandez JM
AD  - Department of Physical and Analytical Chemistry, Faculty of Chemistry, University 
      of Oviedo, Julian Claveria, 8, 33006 Oviedo, Spain. Electronic address: 
      jcostafe@uniovi.es.
FAU - Pereiro, Rosario
AU  - Pereiro R
AD  - Department of Physical and Analytical Chemistry, Faculty of Chemistry, University 
      of Oviedo, Julian Claveria, 8, 33006 Oviedo, Spain. Electronic address: 
      mrpereiro@uniovi.es.
FAU - Sanz-Medel, Alfredo
AU  - Sanz-Medel A
AD  - Department of Physical and Analytical Chemistry, Faculty of Chemistry, University 
      of Oviedo, Julian Claveria, 8, 33006 Oviedo, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150809
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Nanoconjugates)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Antibodies, Monoclonal/chemistry
MH  - Biosensing Techniques/*instrumentation
MH  - Blood Chemical Analysis/*instrumentation
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Fluorescent Dyes/chemical synthesis
MH  - Gold/*chemistry
MH  - Humans
MH  - Immunoassay/*instrumentation
MH  - Immunoglobulin E/*blood
MH  - Metal Nanoparticles/*chemistry/ultrastructure
MH  - Nanoconjugates/chemistry/ultrastructure
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - Competitive immunoassay
OT  - Fluorescence
OT  - Gold nanoclusters
OT  - Human serum
OT  - Immunoglobulin E
EDAT- 2015/11/09 06:00
MHDA- 2016/09/13 06:00
CRDT- 2015/11/09 06:00
PHST- 2015/06/03 00:00 [received]
PHST- 2015/07/30 00:00 [revised]
PHST- 2015/08/08 00:00 [accepted]
PHST- 2015/11/09 06:00 [entrez]
PHST- 2015/11/09 06:00 [pubmed]
PHST- 2016/09/13 06:00 [medline]
AID - S0956-5663(15)30336-5 [pii]
AID - 10.1016/j.bios.2015.08.011 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2016 Mar 15;77:1055-61. doi: 10.1016/j.bios.2015.08.011. 
      Epub 2015 Aug 9.