PMID- 26547579
OWN - NLM
STAT- MEDLINE
DCOM- 20160523
LR  - 20160123
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 161
IP  - 2
DP  - 2016 Feb
TI  - Protein kinase R (PKR) plays a pro-viral role in porcine reproductive and 
      respiratory syndrome virus (PRRSV) replication by modulating viral gene 
      transcription.
PG  - 327-33
LID - 10.1007/s00705-015-2671-0 [doi]
AB  - Protein kinase R (PKR) is involved in apoptotic cell death and antiviral 
      activities in response to many virus infections. To reveal the role of PKR in the 
      replication of porcine reproductive and respiratory syndrome virus (PRRSV), we 
      first examined the kinetics of PKR phosphorylation during PRRSV infection. The 
      results showed that PRRSV transiently activates PKR at 12 and 24 h postinfection. 
      Surprisingly, eIF-2alpha, the well-known downstream target of PKR, was significantly 
      phosphorylated compared to mock-infected cells only at 48 and 72 h postinfection. 
      Reduced viral gene transcription, viral protein synthesis, and virus titer were 
      detected in cells transfected with PKR silencing RNA prior to PRRSV infection 
      compared to control silencing RNA transfected cells, indicating a role of PKR in 
      facilitating virus replication. Overall, our data suggest that PKR is not a major 
      contributor to the phosphorylation of eIF-2alpha during PRRSV infection, but it plays 
      a pro-viral role in PRRSV replication by modulating primarily viral gene 
      transcription.
FAU - Wang, Xiuqing
AU  - Wang X
AUID- ORCID: 0000-0003-0491-4937
AD  - Department of Biology and Microbiology, South Dakota State University, Brookings, 
      SD, 57007, USA. xiuqing.wang@sdstate.edu.
FAU - Zhang, Hanmo
AU  - Zhang H
AD  - Department of Biology and Microbiology, South Dakota State University, Brookings, 
      SD, 57007, USA.
FAU - Abel, Alex M
AU  - Abel AM
AD  - Department of Biology and Microbiology, South Dakota State University, Brookings, 
      SD, 57007, USA.
FAU - Nelson, Eric
AU  - Nelson E
AD  - Department of Veterinary and Biomedical Sciences, South Dakota State University, 
      Brookings, SD, 57007, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20151107
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (eIF-2 Kinase)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Epithelial Cells/virology
MH  - Haplorhini
MH  - *Host-Pathogen Interactions
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Protein Kinases
MH  - *Transcription, Genetic
MH  - *Virus Replication
MH  - eIF-2 Kinase/*metabolism
EDAT- 2015/11/09 06:00
MHDA- 2016/05/24 06:00
CRDT- 2015/11/09 06:00
PHST- 2015/07/21 00:00 [received]
PHST- 2015/10/31 00:00 [accepted]
PHST- 2015/11/09 06:00 [entrez]
PHST- 2015/11/09 06:00 [pubmed]
PHST- 2016/05/24 06:00 [medline]
AID - 10.1007/s00705-015-2671-0 [pii]
AID - 10.1007/s00705-015-2671-0 [doi]
PST - ppublish
SO  - Arch Virol. 2016 Feb;161(2):327-33. doi: 10.1007/s00705-015-2671-0. Epub 2015 Nov 
      7.