PMID- 26547711
OWN - NLM
STAT- MEDLINE
DCOM- 20160512
LR  - 20151109
IS  - 1735-1502 (Print)
IS  - 1735-1502 (Linking)
VI  - 14
IP  - 4
DP  - 2015 Aug
TI  - Constructing Chimeric Antigen for Precise Screening of HTLV-I Infection.
PG  - 427-36
AB  - Individual preparation of two human T-cell lymphotropic virus type I (HTLV-I) 
      diagnostic GST fused peptides (MTA-1 and GD21) is time-consuming and expensive. 
      The aim of this study was to design a novel single chimeric antigen (SCA) to 
      obviate separate expression of proteins and reduce the cost of reagent 
      preparation. Structural protein fragments, including immunodominant B cell linear 
      epitopes, were selected and different SCAs were designed. Tertiary structure, 
      epitope exposure, solubility and stability were calculated for each SCA and 
      compared with each other. The synthetic DNA encoding the interested SCA was 
      sub-cloned into pET32a expression vector, expressed as a soluble form in 
      Escherichia coli BL21 (DE3) cells and purified under native condition using 
      affinity chromatography. The SDS-PAGE results indicated that thioredoxin-fused 
      SCA was successfully expressed as a soluble form in E. coli BL21 (DE3) cells. The 
      results of ELISA confirmed that SCA reacted with anti-HTLV-I antibodies in a 
      concentration-dependent manner. Our results indicated that the designed SCA may 
      be a good candidate for the screening of HTLV-I carriers with 
      antigen-antibody-based tests.
FAU - Heydari Zarnagh, Hafez
AU  - Heydari Zarnagh H
AD  - Department of Clinical Biochemistry, Faculty of Medicine, Sabzevar University of 
      Medical Science, Sabzevar, Iran.
FAU - Hassanpour, Kazem
AU  - Hassanpour K
AD  - Department of Pediatrics, Faculty of Medicine, Sabzevar University of Medical 
      Science, Sabzevar, Iran.
FAU - Rasaee, Mohammad Javad
AU  - Rasaee MJ
AD  - Department of Biotechnology, Faculty of Medical Science, Tarbiat Modares 
      University, Tehran, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Allergy Asthma Immunol
JT  - Iranian journal of allergy, asthma, and immunology
JID - 101146178
RN  - 0 (Antigens, Viral)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Antigens, Viral/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - HTLV-I Infections/*diagnosis
MH  - Human T-lymphotropic virus 1/*immunology
MH  - Humans
MH  - Plasmids
MH  - Recombinant Fusion Proteins/*immunology
MH  - Solubility
OTO - NOTNLM
OT  - Antigenicity
OT  - Chimeric protein
OT  - ELISA
OT  - HTLV-I
EDAT- 2015/11/10 06:00
MHDA- 2016/05/14 06:00
CRDT- 2015/11/09 06:00
PHST- 2015/10/20 00:00 [accepted]
PHST- 2015/11/09 06:00 [entrez]
PHST- 2015/11/10 06:00 [pubmed]
PHST- 2016/05/14 06:00 [medline]
PST - ppublish
SO  - Iran J Allergy Asthma Immunol. 2015 Aug;14(4):427-36.