PMID- 26548659
OWN - NLM
STAT- MEDLINE
DCOM- 20160926
LR  - 20211203
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 13
IP  - 1
DP  - 2016 Jan
TI  - NMDA receptors promote neurogenesis in the neonatal rat subventricular zone 
      following hypoxic‑ischemic injury.
PG  - 206-12
LID - 10.3892/mmr.2015.4501 [doi]
AB  - Evidence suggests the involvement of N‑methyl‑D‑ aspartate receptors (NMDAR) in 
      the regulation of neurogenesis. Functional properties of NMDAR are strongly 
      influenced by the type of NR2 subunits in the receptor complex. NR2A‑ and 
      NR2B‑containing receptors are expressed in neonatal forebrain regions, such as 
      the subventricular zone (SVZ). The aim of the present study was to examine the 
      effect of the protein expression of hypoxic‑ischemic injury NMDAR subunits 2A and 
      2B in the SVZ of neonatal rats. Expression of these and other proteins of 
      interest was performed using immunohistochemistry. The results showed that NR2A 
      expression was decreased at 6 h after hypoxic‑ischemic injury. By contrast, a 
      significant increase in NR2B expression was observed at 24 h after 
      hypoxic‑ischemic injury, induced by the clamping of the right common carotid 
      artery. The functional effect of NMDAR subunits on neurogenesis was also examined 
      by quantifying Nestin and doublecortin (DCX), the microtubule‑associated protein 
      expressed only in immature neurons. In addition, the effects of selective 
      non‑competitive NMDAR antagonist MK‑801 (0.5 mg/kg), NR2B antagonist Ro25‑6981 (5 
      mg/kg), and NR2A antagonist NVP‑AAM077 (5 mg/kg) administered 30 min prior to the 
      hypoxic‑ischemic injury were examined. The number of Nestin‑ and DCX‑positive 
      cells increased significantly 48 h after hypoxic‑ischemic injury, which was 
      reverted by the MK‑801 and Ro25‑6981 antagonists. Notably, NVP‑AAM077 had no 
      significant effect on the expression of Nestin and DCX. In conclusion, the 
      results of the present study demonstrate that hypoxia‑ischemia inhibited the 
      expression of NR2A, but promoted the expression of NR2B. Furthermore, NMDAR 
      promoted neurogenesis in the SVZ of neonatal brains.
FAU - Lai, Qingwei
AU  - Lai Q
AD  - Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 
      Jiangsu 221002, P.R. China.
FAU - Hu, Peng
AU  - Hu P
AD  - Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 
      Jiangsu 221002, P.R. China.
FAU - Li, Qingyun
AU  - Li Q
AD  - Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 
      Jiangsu 221002, P.R. China.
FAU - Li, Xinyu
AU  - Li X
AD  - Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 
      Jiangsu 221002, P.R. China.
FAU - Yuan, Rui
AU  - Yuan R
AD  - Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 
      Jiangsu 221002, P.R. China.
FAU - Tang, Xiaohong
AU  - Tang X
AD  - Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 
      Jiangsu 221002, P.R. China.
FAU - Wang, Wei
AU  - Wang W
AD  - Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 
      Jiangsu 221002, P.R. China.
FAU - Li, Xiaoquan
AU  - Li X
AD  - Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 
      Jiangsu 221002, P.R. China.
FAU - Fan, Hongbin
AU  - Fan H
AD  - Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 
      Jiangsu 221002, P.R. China.
FAU - Yin, Xiaoxing
AU  - Yin X
AD  - Department of Clinical Pharmacology, School of Pharmacy, Xuzhou Medical College, 
      Xuzhou, Jiangsu 221004, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151104
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Dcx protein, rat)
RN  - 0 (Doublecortin Domain Proteins)
RN  - 0 (Doublecortin Protein)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Nestin)
RN  - 0 (Neuropeptides)
RN  - 0 (Protein Subunits)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain Ischemia/*complications/pathology
MH  - Cell Count
MH  - Doublecortin Domain Proteins
MH  - Doublecortin Protein
MH  - Female
MH  - Hypoxia/*complications/pathology
MH  - Immunohistochemistry
MH  - Lateral Ventricles/*metabolism/*pathology
MH  - Male
MH  - Microtubule-Associated Proteins/metabolism
MH  - Nestin/metabolism
MH  - *Neurogenesis
MH  - Neuropeptides/metabolism
MH  - Protein Subunits/metabolism
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors/*metabolism
PMC - PMC4686072
EDAT- 2015/11/10 06:00
MHDA- 2016/09/27 06:00
PMCR- 2015/11/04
CRDT- 2015/11/10 06:00
PHST- 2015/08/21 00:00 [received]
PHST- 2015/11/02 00:00 [accepted]
PHST- 2015/11/10 06:00 [entrez]
PHST- 2015/11/10 06:00 [pubmed]
PHST- 2016/09/27 06:00 [medline]
PHST- 2015/11/04 00:00 [pmc-release]
AID - mmr-13-01-0206 [pii]
AID - 10.3892/mmr.2015.4501 [doi]
PST - ppublish
SO  - Mol Med Rep. 2016 Jan;13(1):206-12. doi: 10.3892/mmr.2015.4501. Epub 2015 Nov 4.