PMID- 26548707
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20220129
IS  - 1931-8405 (Electronic)
IS  - 0889-2229 (Linking)
VI  - 32
IP  - 3
DP  - 2016 Mar
TI  - Macrophage Inflammatory Protein-1 Beta and Interferon Gamma Responses in Ugandans 
      with HIV-1 Acute/Early Infections.
PG  - 237-46
LID - 10.1089/AID.2015.0157 [doi]
AB  - Control of HIV replication through CD4(+) and CD8(+) T cells might be possible, 
      but the functional and phenotypic characteristics of such cells are not defined. 
      Among cytokines produced by T cells, CCR5 ligands, including macrophage 
      inflammatory protein-1 beta (MIP-1beta), compete for the CCR5 coreceptor with HIV, 
      promoting CCR5 internalization and decreasing its availability for virus binding. 
      Interferon (IFN)-gamma also has some antiviral activity and has been used as a 
      read-out for T cell immunogenicity. We used cultured ELISpot assays to compare 
      the relative contribution of MIP-1beta and IFN-gamma to HIV-specific responses. The 
      magnitude of responses was 1.36 times higher for MIP-1beta compared to IFN-gamma. The 
      breadth of the MIP-1beta response (45.41%) was significantly higher than IFN-gamma 
      (36.88%), with considerable overlap between the peptide pools that stimulated 
      both MIP-1beta and IFN-gamma production. Subtype A and D cross-reactive responses were 
      observed both at stimulation and test level, but MIP-1beta and IFN-gamma responses 
      displayed different effect patterns. We conclude that the MIP-1beta ELISpot would be 
      a useful complement to the evaluation of the immunogenicity of HIV vaccines and 
      the activity of adjuvants.
FAU - Obuku, Andrew Ekii
AU  - Obuku AE
AD  - Medical Research Council/Uganda Virus Research Institute , Uganda Research Unit 
      on AIDS, Entebbe, Uganda .
FAU - Bugembe, Daniel L
AU  - Bugembe DL
AD  - Medical Research Council/Uganda Virus Research Institute , Uganda Research Unit 
      on AIDS, Entebbe, Uganda .
FAU - Musinguzi, Kenneth
AU  - Musinguzi K
AD  - Medical Research Council/Uganda Virus Research Institute , Uganda Research Unit 
      on AIDS, Entebbe, Uganda .
FAU - Watera, Christine
AU  - Watera C
AD  - Medical Research Council/Uganda Virus Research Institute , Uganda Research Unit 
      on AIDS, Entebbe, Uganda .
FAU - Serwanga, Jennifer
AU  - Serwanga J
AD  - Medical Research Council/Uganda Virus Research Institute , Uganda Research Unit 
      on AIDS, Entebbe, Uganda .
FAU - Ndembi, Nicaise
AU  - Ndembi N
AD  - Medical Research Council/Uganda Virus Research Institute , Uganda Research Unit 
      on AIDS, Entebbe, Uganda .
FAU - Levin, Jonathan
AU  - Levin J
AD  - Medical Research Council/Uganda Virus Research Institute , Uganda Research Unit 
      on AIDS, Entebbe, Uganda .
FAU - Kaleebu, Pontiano
AU  - Kaleebu P
AD  - Medical Research Council/Uganda Virus Research Institute , Uganda Research Unit 
      on AIDS, Entebbe, Uganda .
FAU - Pala, Pietro
AU  - Pala P
AD  - Medical Research Council/Uganda Virus Research Institute , Uganda Research Unit 
      on AIDS, Entebbe, Uganda .
LA  - eng
GR  - MC_UP_A910_1025/MRC_/Medical Research Council/United Kingdom
GR  - WT078927MA/WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151209
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (Chemokine CCL4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Cells, Cultured
MH  - Chemokine CCL4/*metabolism
MH  - Enzyme-Linked Immunospot Assay
MH  - Female
MH  - HIV Infections/*immunology/virology
MH  - HIV-1/*immunology/isolation & purification
MH  - Humans
MH  - Interferon-gamma/*metabolism
MH  - Leukocytes, Mononuclear/*immunology
MH  - Male
MH  - Middle Aged
MH  - Prospective Studies
MH  - Uganda
EDAT- 2015/11/10 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/11/10 06:00
PHST- 2015/11/10 06:00 [entrez]
PHST- 2015/11/10 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1089/AID.2015.0157 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 2016 Mar;32(3):237-46. doi: 10.1089/AID.2015.0157. 
      Epub 2015 Dec 9.