PMID- 26549829
OWN - NLM
STAT- MEDLINE
DCOM- 20161024
LR  - 20161230
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 228
DP  - 2016 Feb
TI  - A simple cytogenetic method to detect chromosomally integrated human 
      herpesvirus-6.
PG  - 74-8
LID - S0166-0934(15)00355-9 [pii]
LID - 10.1016/j.jviromet.2015.11.001 [doi]
AB  - Some healthy individuals carry human herpesvirus-6 (HHV-6) within a host 
      chromosome, which is called inherited chromosomally integrated human 
      herpesvirus-6 (iciHHV-6). Because iciHHV-6 is generally considered a 
      non-pathogenic condition, it is important to distinguish iciHHV-6 from HHV-6 
      reactivation in immunocompromised hosts because both conditions manifest high 
      copy numbers of the HHV-6 in peripheral blood mononuclear cells. Although 
      fluorescent in situ hybridization (FISH) is a reliable method for the diagnosis 
      of iciHHV-6, HHV-6-specific FISH probes are not commercially available. In our 
      present study, we established a simple PCR-based method for producing FISH probes 
      that can detect the chromosomal integration site of iciHHV-6 at high sensitivity. 
      Using these probes, we confirmed that HHV-6 signals were consistently located at 
      the telomeric region in all of the 13 iciHHV-6 individuals examined. 
      Interestingly, in all seven Japanese iciHHV-6A patients, signals were detected 
      exclusively on chromosome 22q. This method provides a simple and fast approach 
      for iciHHV-6 diagnosis in the clinical laboratory.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Ohye, Tamae
AU  - Ohye T
AD  - Division of Molecular Genetics, Institute for Comprehensive Medical Science, 
      Fujita Health University, Toyoake, Aichi 470-1192, Japan; Department of Clinical 
      Hematology, Faculty of Medical Technology, Fujita Health University, Toyoake, 
      Aichi 470-1192, Japan.
FAU - Kawamura, Yoshiki
AU  - Kawamura Y
AD  - Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, 
      Aichi 470-1192, Japan.
FAU - Inagaki, Hidehito
AU  - Inagaki H
AD  - Division of Molecular Genetics, Institute for Comprehensive Medical Science, 
      Fujita Health University, Toyoake, Aichi 470-1192, Japan.
FAU - Yoshikawa, Akiko
AU  - Yoshikawa A
AD  - Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, 
      Aichi 470-1192, Japan.
FAU - Ihira, Masaru
AU  - Ihira M
AD  - Faculty of Clinical Engineering, Fujita Health University School of Health 
      Sciences, Toyoake, Aichi 470-1192, Japan.
FAU - Yoshikawa, Tetsushi
AU  - Yoshikawa T
AD  - Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, 
      Aichi 470-1192, Japan.
FAU - Kurahashi, Hiroki
AU  - Kurahashi H
AD  - Division of Molecular Genetics, Institute for Comprehensive Medical Science, 
      Fujita Health University, Toyoake, Aichi 470-1192, Japan. Electronic address: 
      kura@fujita-hu.ac.jp.
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151106
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (DNA, Viral)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Cytogenetic Analysis/*methods
MH  - DNA, Viral/genetics
MH  - Herpesvirus 6, Human/*genetics/*isolation & purification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Oligonucleotide Probes
MH  - Polymerase Chain Reaction/methods
MH  - Sensitivity and Specificity
MH  - *Virus Integration
OTO - NOTNLM
OT  - Chromosomally integrated human herpesvirus-6
OT  - Chromosome
OT  - FISH
OT  - HHV-6 probe
OT  - PCR-based method
OT  - Telomere
EDAT- 2015/11/10 06:00
MHDA- 2016/10/25 06:00
CRDT- 2015/11/10 06:00
PHST- 2015/04/28 00:00 [received]
PHST- 2015/11/02 00:00 [revised]
PHST- 2015/11/03 00:00 [accepted]
PHST- 2015/11/10 06:00 [entrez]
PHST- 2015/11/10 06:00 [pubmed]
PHST- 2016/10/25 06:00 [medline]
AID - S0166-0934(15)00355-9 [pii]
AID - 10.1016/j.jviromet.2015.11.001 [doi]
PST - ppublish
SO  - J Virol Methods. 2016 Feb;228:74-8. doi: 10.1016/j.jviromet.2015.11.001. Epub 
      2015 Nov 6.