PMID- 26550219
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151109
LR  - 20200930
IS  - 1940-5901 (Print)
IS  - 1940-5901 (Electronic)
IS  - 1940-5901 (Linking)
VI  - 8
IP  - 8
DP  - 2015
TI  - Effects of combined thymosin and hydrocortisone on immune response in septic 
      mice.
PG  - 12989-94
AB  - This study is to investigate the effects of the thymosin alpha1 (Talpha1) and 
      hydrocortisone (HC) combination treatment on the immune responses in septic mice. 
      According to different treatments, mice were divided into the control group (n = 
      18), the sepsis model group (n = 18), the Talpha1 group (n = 18), the HC group (n = 
      18), and the Talpha1+HC group (n = 18). Septic mouse model was established by the 
      intraperitoneal injection of lipopolysaccharide (LPS). At 72 h after modeling, 
      flow cytometry was used to analyze the dendritic cell (DC) numbers in peripheral 
      blood and the expressions of MHC II and CD86. Tumor necrosis factor-alpha (TNF-alpha) 
      level was measured by ELISA. Treatments of Talpha1 and/or HC dramatically increased 
      the survival rates of LPS-induced septic mice. Flow cytometry showed that, the DC 
      numbers in peripheral blood were significantly decreased in the sepsis model 
      group, which could be dramatically elevated by Talpha1 treatment alone and in 
      combination with HC (Talpha1+HC). However, the DCs were undetectable in the HC group. 
      In addition, the MHC II expression level was decreased in the sepsis model group, 
      which was further declined in the Talpha1 and Talpha1+HC groups. The expression level of 
      CD86 was elevated in the model group, which could be significantly down-regulated 
      by the treatments of Talpha1 and Talpha1+HC. ELISA showed that, the peripheral blood 
      TNF-alpha level in the HC group was lower than in the sepsis model group. Compared 
      with the sepsis model group, the TNF-alpha levels were significantly elevated in the 
      Talpha1 and Talpha1+HC groups. Talpha1 and HC combination treatment could improve the immune 
      function and regulate the inflammatory response to increase the survival rates of 
      LPS-induced septic mice.
FAU - Zhang, Daquan
AU  - Zhang D
AD  - The Second Department of Critical Care Medicine, People's Hospital of Xinjiang 
      Uygur Autonomous Region Urumqi 830001, Xinjiang, China.
FAU - Zhou, Yi
AU  - Zhou Y
AD  - Intensive Care Unit, The Eleventh Hospital of PLA Yining 835000, Xinjiang, China.
FAU - Cheng, Qinghong
AU  - Cheng Q
AD  - Department of Critical Care Medicine, The First Affiliated Hospital of Shihezi 
      University Shihezi 832000, Xinjiang, China.
LA  - eng
PT  - Journal Article
DEP - 20150815
PL  - United States
TA  - Int J Clin Exp Med
JT  - International journal of clinical and experimental medicine
JID - 101471010
PMC - PMC4612904
OTO - NOTNLM
OT  - Sepsis
OT  - dendritic cells
OT  - hydrocortisone
OT  - immune response
OT  - thymosin
EDAT- 2015/11/10 06:00
MHDA- 2015/11/10 06:01
PMCR- 2015/08/15
CRDT- 2015/11/10 06:00
PHST- 2015/05/13 00:00 [received]
PHST- 2015/07/03 00:00 [accepted]
PHST- 2015/11/10 06:00 [entrez]
PHST- 2015/11/10 06:00 [pubmed]
PHST- 2015/11/10 06:01 [medline]
PHST- 2015/08/15 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Med. 2015 Aug 15;8(8):12989-94. eCollection 2015.