PMID- 26550451
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151109
LR  - 20200930
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 7
IP  - 9
DP  - 2015
TI  - Silencing c-Kit expression in human DCs suppresses Th2, Th17 response but 
      enhances Th1 response.
PG  - 1499-509
AB  - Dendritic cells (DCs) are integral to the differentiation of T helper cells into 
      T helper type 1 TH1, TH2 and TH17 subsets. RNA interference (RNAi), which causes 
      the degradation of any RNA in a sequence specific manner, is a 
      posttranscriptional gene silencing mechanism. Targeting the c-Kit in DCs has been 
      used as an approach to enhance antitumor immunity. Here, we shwed that 
      transfection of DCs with siRNA specific for c-Kit gene can significantly knock 
      down c-Kit. When exposed to TNF-alpha, immature DCs transfected with c-Kit siRNA can 
      differentiate into mature DCs without reducing viability or IL-12p70 production. 
      The c-Kit siRNA-treated DCs exhibited an increased allostimulatory capacity in a 
      lymphocyte proliferation assay. Furthermore, c-Kit siRNA-transfected DCs enhanced 
      TH1 responses by increasing IFN-gamma and decreasing IL-4 production, and much 
      stronger cytotoxic activity was observed when DCs were co-transfected with c-Kit 
      siRNA and an endogenous tumor antigen in vitro. Our findings indicate that 
      silencing the c-Kit gene in DCs with siRNA may offer a potential approach to 
      enhance antitumor immunotherapy.
FAU - Yang, Bin
AU  - Yang B
AD  - Department of Dermatology, Wuhan General Hospital of Guangzhou Command Wuluo Road 
      627, Wuhan 430070, Hubei Province, China.
FAU - Yang, Qin
AU  - Yang Q
AD  - Department of Medical Laboratory, Wuhan General Hospital of Guangzhou Command 
      Wuluo Road 627, Wuhan 430070, Hubei Province, China.
FAU - Huang, Qianchuan
AU  - Huang Q
AD  - Department of Medical Laboratory, Wuhan General Hospital of Guangzhou Command 
      Wuluo Road 627, Wuhan 430070, Hubei Province, China.
FAU - Yan, Hongbo
AU  - Yan H
AD  - Department of Dermatology, Wuhan General Hospital of Guangzhou Command Wuluo Road 
      627, Wuhan 430070, Hubei Province, China.
FAU - Sun, Ting
AU  - Sun T
AD  - Department of Dermatology, Wuhan General Hospital of Guangzhou Command Wuluo Road 
      627, Wuhan 430070, Hubei Province, China.
FAU - Tong, Hui
AU  - Tong H
AD  - Department of Dermatology, Wuhan General Hospital of Guangzhou Command Wuluo Road 
      627, Wuhan 430070, Hubei Province, China.
LA  - eng
PT  - Journal Article
DEP - 20150915
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC4626413
OTO - NOTNLM
OT  - Small interfering RNA
OT  - anti-tumor immunity
OT  - c-Kit
OT  - dendritic cells
EDAT- 2015/11/10 06:00
MHDA- 2015/11/10 06:01
PMCR- 2015/09/15
CRDT- 2015/11/10 06:00
PHST- 2015/07/07 00:00 [received]
PHST- 2015/09/13 00:00 [accepted]
PHST- 2015/11/10 06:00 [entrez]
PHST- 2015/11/10 06:00 [pubmed]
PHST- 2015/11/10 06:01 [medline]
PHST- 2015/09/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2015 Sep 15;7(9):1499-509. eCollection 2015.