PMID- 26550465
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151109
LR  - 20200930
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 7
IP  - 9
DP  - 2015
TI  - Biological roles of human bone morphogenetic protein 9 in the bone 
      microenvironment of human breast cancer MDA-MB-231 cells.
PG  - 1660-74
AB  - Bone marrow stroma plays a critical role in the bone metastasis of breast cancer. 
      Bone marrow-derived mesenchymal stem cells (BMSC) are critical to facilitate 
      cancer progression. Human bone morphogenetic protein 9 (BMP9) is the most potent 
      osteogenic factor and one of bone-stored growth factors involved in both 
      promotion and inhibition of different cancers. However, it is unclear whether 
      BMP9 correlates with the bone metastasis of breast cancer. This study was to 
      evaluate the role of BMP9 in the interaction between BMSC and breast cancer cells 
      (BCC). To determine whether BMP9 is able to block the tumor promoting effect of 
      BMSC, an in vitro model was developed using breast cancer MDA-MB-231 cells 
      co-cultured with bone marrow-derived mesenchymal stem cells HS-5 with-BMP9 
      overexpression. The expressions of metastasis-related genes were detected to 
      identify important factors mediating the role of BMP9 in breast cancer cells. 
      Results showed BMP9 could inhibit invasion and promote apoptosis of MDA-MB-231 
      cells. The expressions of interleukin-6 (IL-6), matrix metalloproteinase-2 
      (MMP-2) and monocyte chemoattratctant protein-1 (MCP-1) decreased in the 
      MDA-MB-231 cells of BMP9 over-expression group, and the expressions of 
      epithelial-mesenchymal transition (EMT)-related molecules was also reduced. On 
      the other hand, the expression of stromal cell derived factor-1 (SDF-1) decreased 
      in HS-5 cells of BMP9 over-expression group. Taken together, BMP9 is able to 
      inhibit the migration and promote the apoptosis of breast cancer by regulating 
      the interaction between MDA-MB-231 cells and HS-5 cells in which SDF-1/CXCR4-PI3K 
      pathway and EMT are involved.
FAU - Wang, Wei
AU  - Wang W
AD  - Key Laboratory of Diagnostic Medicine of The Chinese Ministry of Education, 
      School of Clinical Diagnostic and Laboratory Medicine, Chongqing Medical 
      University No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China.
FAU - Weng, Yaguang
AU  - Weng Y
AD  - Key Laboratory of Diagnostic Medicine of The Chinese Ministry of Education, 
      School of Clinical Diagnostic and Laboratory Medicine, Chongqing Medical 
      University No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China.
FAU - Ren, Wei
AU  - Ren W
AD  - Department of General Surgery, The First Affiliated Hospital of Chongqing Medical 
      University No.1 Youyi Road, Yuzhong District, Chongqing 400042, China.
FAU - Zhang, Zhihui
AU  - Zhang Z
AD  - Key Laboratory of Diagnostic Medicine of The Chinese Ministry of Education, 
      School of Clinical Diagnostic and Laboratory Medicine, Chongqing Medical 
      University No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China.
FAU - Wang, Ting
AU  - Wang T
AD  - Key Laboratory of Diagnostic Medicine of The Chinese Ministry of Education, 
      School of Clinical Diagnostic and Laboratory Medicine, Chongqing Medical 
      University No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China.
FAU - Wang, Jinshu
AU  - Wang J
AD  - Key Laboratory of Diagnostic Medicine of The Chinese Ministry of Education, 
      School of Clinical Diagnostic and Laboratory Medicine, Chongqing Medical 
      University No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China.
FAU - Jiang, Yayun
AU  - Jiang Y
AD  - Key Laboratory of Diagnostic Medicine of The Chinese Ministry of Education, 
      School of Clinical Diagnostic and Laboratory Medicine, Chongqing Medical 
      University No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China.
FAU - Chen, Yingying
AU  - Chen Y
AD  - Key Laboratory of Diagnostic Medicine of The Chinese Ministry of Education, 
      School of Clinical Diagnostic and Laboratory Medicine, Chongqing Medical 
      University No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China.
FAU - Zhou, Lan
AU  - Zhou L
AD  - Key Laboratory of Diagnostic Medicine of The Chinese Ministry of Education, 
      School of Clinical Diagnostic and Laboratory Medicine, Chongqing Medical 
      University No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China.
FAU - He, Tongchuan
AU  - He T
AD  - Molecular Oncology Laboratory, Department of Surgery, University of Chicago 
      Medical Center Chicago, IL, USA.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Key Laboratory of Diagnostic Medicine of The Chinese Ministry of Education, 
      School of Clinical Diagnostic and Laboratory Medicine, Chongqing Medical 
      University No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China.
LA  - eng
PT  - Journal Article
DEP - 20150915
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC4626427
OTO - NOTNLM
OT  - Bone morphogenetic protein 9
OT  - SDF-1/CXCR4-PI3K pathway
OT  - bone metastasis
OT  - breast cancer
OT  - epithelial-mesenchymal transition
EDAT- 2015/11/10 06:00
MHDA- 2015/11/10 06:01
PMCR- 2015/09/15
CRDT- 2015/11/10 06:00
PHST- 2015/07/01 00:00 [received]
PHST- 2015/08/24 00:00 [accepted]
PHST- 2015/11/10 06:00 [entrez]
PHST- 2015/11/10 06:00 [pubmed]
PHST- 2015/11/10 06:01 [medline]
PHST- 2015/09/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2015 Sep 15;7(9):1660-74. eCollection 2015.