PMID- 26550565
OWN - NLM
STAT- MEDLINE
DCOM- 20160803
LR  - 20240325
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2015
DP  - 2015
TI  - Bone regeneration by nanohydroxyapatite/chitosan/poly(lactide-co-glycolide) 
      scaffolds seeded with human umbilical cord mesenchymal stem cells in the 
      calvarial defects of the nude mice.
PG  - 261938
LID - 10.1155/2015/261938 [doi]
LID - 261938
AB  - In the preliminary study, we have found an excellent osteogenic property of 
      nanohydroxyapatite/chitosan/poly(lactide-co-glycolide) (nHA/CS/PLGA) scaffolds 
      seeded with human umbilical cord mesenchymal stem cells (hUCMSCs) in vitro and 
      subcutaneously in the nude mice. The aim of this study was to further evaluate 
      the osteogenic capacity of nHA/CS/PLGA scaffolds seeded with hUCMSCs in the 
      calvarial defects of the nude mice. Totally 108 nude mice were included and 
      divided into 6 groups: PLGA scaffolds + hUCMSCs; nHA/PLGA scaffolds + hUCMSCs; 
      CS/PLGA scaffolds + hUCMSCs; nHA/CS/PLGA scaffolds + hUCMSCs; nHA/CS/PLGA 
      scaffolds without seeding; the control group (no scaffolds) (n = 18). The 
      scaffolds were implanted into the calvarial defects of nude mice. The amount of 
      new bones was evaluated by fluorescence labeling, H&E staining, and Van Gieson 
      staining at 4 and 8 weeks, respectively. The results demonstrated that the amount 
      of new bones was significantly increased in the group of nHA/CS/PLGA scaffolds 
      seeded with hUCMSCs (p < 0.01). On the basis of previous studies in vitro and in 
      subcutaneous implantation of the nude mice, the results revealed that the nHA and 
      CS also enhanced the bone regeneration by nHA/CS/PLGA scaffolds seeded with 
      hUCMSCs in the calvarial defects of the nude mice at early stage.
FAU - Wang, Fei
AU  - Wang F
AD  - Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an 
      Jiaotong University, Xi'an 710004, China.
FAU - Su, Xiao-Xia
AU  - Su XX
AD  - Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an 
      Jiaotong University, Xi'an 710004, China.
FAU - Guo, Yu-Cheng
AU  - Guo YC
AD  - Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an 
      Jiaotong University, Xi'an 710004, China.
FAU - Li, Ang
AU  - Li A
AD  - Research Center for Stomatology, Stomatological Hospital, College of Medicine, 
      Xi'an Jiaotong University, Xi'an 710004, China.
FAU - Zhang, Yin-Cheng
AU  - Zhang YC
AD  - Department of Oral and Maxillofacial Surgery, Stomatological Hospital, College of 
      Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an 
      Jiaotong University, Xi'an 710004, China.
FAU - Qiao, Hu
AU  - Qiao H
AD  - Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an 
      Jiaotong University, Xi'an 710004, China.
FAU - Guan, Li-Min
AU  - Guan LM
AD  - Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an 
      Jiaotong University, Xi'an 710004, China.
FAU - Zou, Min
AU  - Zou M
AD  - Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an 
      Jiaotong University, Xi'an 710004, China.
FAU - Si, Xin-Qin
AU  - Si XQ
AD  - Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an 
      Jiaotong University, Xi'an 710004, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151013
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9012-76-4 (Chitosan)
RN  - 91D9GV0Z28 (Durapatite)
SB  - IM
MH  - Animals
MH  - Bone Regeneration/*physiology
MH  - Chitosan/chemistry
MH  - Cord Blood Stem Cell Transplantation/*instrumentation/methods
MH  - Durapatite/chemistry
MH  - Equipment Failure Analysis
MH  - Humans
MH  - Lactic Acid/chemistry
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*instrumentation
MH  - Mice
MH  - Mice, Nude
MH  - Nanocomposites/chemistry
MH  - Polyglycolic Acid/chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Prosthesis Design
MH  - Skull Fractures/*pathology/physiopathology/*surgery
MH  - *Tissue Scaffolds
MH  - Treatment Outcome
PMC - PMC4621339
EDAT- 2015/11/10 06:00
MHDA- 2016/08/04 06:00
PMCR- 2015/10/13
CRDT- 2015/11/10 06:00
PHST- 2015/05/25 00:00 [received]
PHST- 2015/09/11 00:00 [revised]
PHST- 2015/09/15 00:00 [accepted]
PHST- 2015/11/10 06:00 [entrez]
PHST- 2015/11/10 06:00 [pubmed]
PHST- 2016/08/04 06:00 [medline]
PHST- 2015/10/13 00:00 [pmc-release]
AID - 10.1155/2015/261938 [doi]
PST - ppublish
SO  - Biomed Res Int. 2015;2015:261938. doi: 10.1155/2015/261938. Epub 2015 Oct 13.