PMID- 26550577
OWN - NLM
STAT- MEDLINE
DCOM- 20160802
LR  - 20181113
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2015
DP  - 2015
TI  - Glutamine may repress the weak LPS and enhance the strong heat shock induction of 
      monocyte and lymphocyte HSP72 proteins but may not modulate the HSP72 mRNA in 
      patients with sepsis or trauma.
PG  - 806042
LID - 10.1155/2015/806042 [doi]
LID - 806042
AB  - OBJECTIVE: We assessed the lipopolysaccharide (LPS) or heat shock (HS) induction 
      of heat shock protein-72 (HSP72) in peripheral blood mononuclear cells (PBMCs) of 
      patients with severe sepsis (SS) or trauma-related systemic inflammatory response 
      syndrome (SIRS), compared to healthy individuals (H); we also investigated any 
      pre- or posttreatment modulating glutamine (Gln) effect. METHODS: SS (11), SIRS 
      (10), and H (19) PBMCs were incubated with 1 mug/mL LPS or 43 degrees HS. Gln 10 mM was 
      either added 1 h before or 1 h after induction or was not added at all. We 
      measured monocyte (m), lymphocyte (l), mRNA HSP72, HSP72 polymorphisms, 
      interleukins (ILs), monocyte chemoattractant protein-1 (MCP-1), and cortisol 
      levels. RESULTS: Baseline lHSP72 was higher in SS (p < 0.03), and mHSP72 in SIRS 
      (p < 0.02), compared to H. Only HS induced l/mHSP72/mRNA HSP72; LPS induced IL-6, 
      IL-8, IL-10, and MCP-1. Induced mRNA was related to l/mHSP72, and was related 
      negatively to cytokines. Intracellular l/mHSP72/HSP72 mRNA was related to serum 
      ILs, not being influenced by cortisol, illness severity, and HSP72 polymorphisms. 
      Gln did not induce mRNA in any group but modified l/mHSP72 after LPS/HS induction 
      unpredictably. CONCLUSIONS: HSP72 mRNA and l/mHSP72 are higher among critically 
      ill patients, further induced by HS, not by LPS. HSP72 proteins and HSP72 mRNA 
      are related to serum ILs and are negatively related to supernatant cytokines, not 
      being influenced by HSP72 polymorphisms, cortisol, or illness severity. Gln may 
      depress l/mHSP72 after LPS exposure and enhance them after HS induction, but it 
      may not affect early induced HSP72 mRNA.
FAU - Briassouli, Efrossini
AU  - Briassouli E
AD  - First Department of Propaedeutic Internal Medicine, University of Athens, Athens, 
      Greece.
FAU - Tzanoudaki, Marianna
AU  - Tzanoudaki M
AD  - Department of Immunology-Histocompatibility, Specialized Center & Referral Center 
      for Primary Immunodeficiencies-Paediatric Immunology, "Aghia Sophia" Children's 
      Hospital, Athens, Greece.
FAU - Goukos, Dimitris
AU  - Goukos D
AD  - First Department of Propaedeutic Internal Medicine, University of Athens, Athens, 
      Greece.
FAU - Routsi, Christina
AU  - Routsi C
AD  - First Critical Care Department, Evangelismos Hospital, University of Athens, 
      Athens, Greece.
FAU - Nanas, Serafim
AU  - Nanas S
AD  - First Critical Care Department, Evangelismos Hospital, University of Athens, 
      Athens, Greece.
FAU - Vardas, Kostas
AU  - Vardas K
AD  - First Critical Care Department, Evangelismos Hospital, University of Athens, 
      Athens, Greece.
FAU - Apostolou, Kleovoulos
AU  - Apostolou K
AD  - First Critical Care Department, Evangelismos Hospital, University of Athens, 
      Athens, Greece.
FAU - Kanariou, Maria
AU  - Kanariou M
AD  - Department of Immunology-Histocompatibility, Specialized Center & Referral Center 
      for Primary Immunodeficiencies-Paediatric Immunology, "Aghia Sophia" Children's 
      Hospital, Athens, Greece.
FAU - Daikos, George
AU  - Daikos G
AD  - First Department of Propaedeutic Internal Medicine, University of Athens, Athens, 
      Greece.
FAU - Briassoulis, George
AU  - Briassoulis G
AD  - Pediatric Intensive Care Unit, University Hospital, University of Crete, 71500 
      Heraklion, Greece.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151013
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (HSP72 Heat-Shock Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0RH81L854J (Glutamine)
SB  - IM
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Glutamine/*administration & dosage
MH  - HSP72 Heat-Shock Proteins/genetics/*immunology
MH  - Heat-Shock Response
MH  - Humans
MH  - Lipopolysaccharides/administration & dosage
MH  - Lymphocytes/drug effects/*immunology
MH  - Monocytes/drug effects/*immunology
MH  - RNA, Messenger/metabolism
MH  - Sepsis/*immunology/pathology
MH  - Systemic Inflammatory Response Syndrome/*immunology/pathology
MH  - Wounds and Injuries/immunology/pathology
PMC - PMC4621332
EDAT- 2015/11/10 06:00
MHDA- 2016/08/03 06:00
PMCR- 2015/10/13
CRDT- 2015/11/10 06:00
PHST- 2015/06/17 00:00 [received]
PHST- 2015/08/23 00:00 [revised]
PHST- 2015/08/31 00:00 [accepted]
PHST- 2015/11/10 06:00 [entrez]
PHST- 2015/11/10 06:00 [pubmed]
PHST- 2016/08/03 06:00 [medline]
PHST- 2015/10/13 00:00 [pmc-release]
AID - 10.1155/2015/806042 [doi]
PST - ppublish
SO  - Biomed Res Int. 2015;2015:806042. doi: 10.1155/2015/806042. Epub 2015 Oct 13.