PMID- 26552176
OWN - NLM
STAT- MEDLINE
DCOM- 20151217
LR  - 20151110
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 40
IP  - 11
DP  - 2015 Jun
TI  - [Effect of Gualou Xiebai Banxia decoction combined with Xuefu Zhuyu decoction on 
      myocardial apoptosis and relevant protein expressions in miniature swine phlegm 
      and blood stasis type coronary heart disease model].
PG  - 2174-9
AB  - There have been very few studies on the effect of Gualou Xiebai Banxia decoction 
      combined with Xuefu Zhuyu decoction in inhibiting apoptosis in myocardial 
      ischemial injury caused by coronary heart disease. In this experiment, Gualou 
      Xiebai Banxia decoction combined with-Xuefu Zhuyu decoction were used to 
      intervene the miniature swine phlegm and blood stasis type coronary heart disease 
      model, in order to observe the effect of the combined prescription on the 
      myocardial apoptosis and the expressions of Bcl-2, Bax, Caspase-3, Caspase-9 in 
      the model. Totally 15 Chinese experimental miniature swine were adopted and 
      randomly divided into the control group, the model group and the phlegm and 
      stasis-treating group. The model group and the stasis-treating group were fed 
      with high fat diets for two weeks, intervened with the coronary artery injury and 
      then given drugs and high fat diets for eight weeks. The control group was fed 
      with ordinary diets for 10 weeks, without the coronary artery injury. After the 
      experiment, myocardia at the juncture of infracted areas were collected and made 
      into formalin-fixed paraffin sections. The TDT-mediate dUTP nick end labeling 
      (TUNEL) assay was used to detect the myocardial apoptosis. The 
      immunohistochemistry (IHC) technique was applied to detect Bcl-2, Bax, Caspase-3, 
      Caspase-9 levels in myocardial tissues. According to the findings, the apoptosis 
      indexes (AI) for the control group, the model group and the phlegm and 
      stasis-treating group were 0.92%, 27.68%, 17.28%, respectively. The AI of the 
      phlegm and stasis-treating group was significantly lower than that of the model 
      group (P < 0.01). Compared with the model group, the phlegm and stasis-treating 
      group showed significantly higher Bcl-2 protein expression (P < 0.01) and lower 
      Bax, Caspase-3 and Caspase-9 protein expressions (P < 0.01). In conclusion, 
      Gualou Xiebai Banxia decoction combined with Xuefu Zhuyu decoction have a 
      significant protective effect against the myocardial apoptosis in miniature swine 
      phlegm and blood stasis type coronary heart disease model.
FAU - Yan, Ai-guo
AU  - Yan AG
FAU - Liu, Jian-xun
AU  - Liu JX
FAU - Li, Xin-zhi
AU  - Li XZ
FAU - Dong, Xiao-xia
AU  - Dong XX
FAU - Li, Hong-kun
AU  - Li HK
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Xue-Fu-Zhu-Yu decoction)
RN  - 0 (bcl-2-Associated X Protein)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Caspases/metabolism
MH  - Coronary Disease/*drug therapy
MH  - Disease Models, Animal
MH  - Drug Therapy, Combination
MH  - Drugs, Chinese Herbal/*administration & dosage
MH  - Female
MH  - Male
MH  - *Medicine, Chinese Traditional
MH  - Myocardium/pathology
MH  - *Phytotherapy
MH  - Proto-Oncogene Proteins c-bcl-2/analysis
MH  - Swine
MH  - Swine, Miniature
MH  - bcl-2-Associated X Protein/analysis
EDAT- 2015/11/11 06:00
MHDA- 2015/12/19 06:00
CRDT- 2015/11/11 06:00
PHST- 2015/11/11 06:00 [entrez]
PHST- 2015/11/11 06:00 [pubmed]
PHST- 2015/12/19 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2015 Jun;40(11):2174-9.