PMID- 26553563
OWN - NLM
STAT- MEDLINE
DCOM- 20160624
LR  - 20171116
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 168
IP  - 1-2
DP  - 2015 Nov 15
TI  - PD-L1 expression is increased in monocyte derived dendritic cells in response to 
      porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus 
      infections.
PG  - 24-9
LID - S0165-2427(15)00219-6 [pii]
LID - 10.1016/j.vetimm.2015.09.013 [doi]
AB  - Host immune system suppression is thought to be crucial in the development of 
      porcine circovirus associated diseases (PCVAD). Many immune suppressive 
      mechanisms have been studied in cases of PCVAD, however, the role of programmed 
      death ligand-1 (PD-L1) during porcine circovirus type 2 (PCV2) infection and 
      PCVAD development has yet to be determined. PD-L1 has become an important 
      research target because of its ability to interfere with effective T-cell 
      activity and proliferation during the course of an immune response. In this 
      study, porcine monocyte derived dendritic cells (MoDC) were infected with 
      different combinations of PCV2 and porcine reproductive and respiratory syndrome 
      virus (PRRSV) and evaluated for expression levels of PD-L1, as well as the 
      expression levels of swine major histocompatibility complexes 1 and 2 (SLA-1 and 
      SLA-2) as a measure of MoDC stimulatory capacity. PD-L1 expression levels were 
      also tested in MoDCs after treatment with interferon alpha (IFN-alpha) and beta 
      (IFN-beta). The results showed that the expression levels of PD-L1 were increased in 
      PCV2-infected MoDCs, as well as in PCV2 and PRRSV co-infected MoDCs. The MoDCs 
      infected with PRRSV only also showed a strain-dependent increase in PD-L1 
      expression. Both IFN-alpha and IFN-beta treatment also increased the expression levels 
      of PD-L1 in MoDCs. SLA-1 and 2 expression levels were increased by PCV2 
      infection, and altered in the PRRSV, and PCV2+PRRSV co-infected MoDCs in a 
      strain-dependent manner. These results indicate a potential immuno-suppressive 
      role for dendritic cells during PCV2 infection and the development of PCVAD and 
      will be helpful in more fully elucidating the underlying mechanisms leading to 
      clinical PCVAD.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Richmond, O
AU  - Richmond O
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, 
      VA, United States.
FAU - Cecere, T E
AU  - Cecere TE
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, 
      VA, United States. Electronic address: tcecere@vt.edu.
FAU - Erdogan, E
AU  - Erdogan E
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, 
      VA, United States.
FAU - Meng, X J
AU  - Meng XJ
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, 
      VA, United States.
FAU - Pineyro, P
AU  - Pineyro P
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, 
      VA, United States.
FAU - Subramaniam, S
AU  - Subramaniam S
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, 
      VA, United States.
FAU - Todd, S M
AU  - Todd SM
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, 
      VA, United States.
FAU - LeRoith, T
AU  - LeRoith T
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, 
      VA, United States.
LA  - eng
PT  - Journal Article
DEP - 20150928
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (B7-H1 Antigen)
RN  - 0 (Interferon Type I)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - B7-H1 Antigen/genetics/*metabolism
MH  - Circoviridae Infections/genetics/immunology/*veterinary
MH  - *Circovirus
MH  - Dendritic Cells/immunology
MH  - Immune Tolerance
MH  - In Vitro Techniques
MH  - Interferon Type I/administration & dosage
MH  - Major Histocompatibility Complex
MH  - Monocytes/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/genetics/*immunology
MH  - RNA, Messenger/genetics/metabolism
MH  - Sus scrofa
MH  - Swine
MH  - Swine Diseases/genetics/*immunology
MH  - Up-Regulation
OTO - NOTNLM
OT  - Co-infection
OT  - Monocyte derived dendritic cells (MoDC)
OT  - Porcine circovirus associated disease (PCVAD)
OT  - Porcine circovirus type 2 (PCV2)
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Programmed death ligand-1 (PD-L1)
EDAT- 2015/11/11 06:00
MHDA- 2016/06/25 06:00
CRDT- 2015/11/11 06:00
PHST- 2015/06/24 00:00 [received]
PHST- 2015/09/17 00:00 [revised]
PHST- 2015/09/22 00:00 [accepted]
PHST- 2015/11/11 06:00 [entrez]
PHST- 2015/11/11 06:00 [pubmed]
PHST- 2016/06/25 06:00 [medline]
AID - S0165-2427(15)00219-6 [pii]
AID - 10.1016/j.vetimm.2015.09.013 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2015 Nov 15;168(1-2):24-9. doi: 
      10.1016/j.vetimm.2015.09.013. Epub 2015 Sep 28.