PMID- 265540
OWN - NLM
STAT- MEDLINE
DCOM- 19770520
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 74
IP  - 2
DP  - 1977 Feb
TI  - Purification and characterization of the DNA polymerase of human breast cancer 
      particles.
PG  - 764-8
AB  - Previous studies have identified human breast tumor particles possessing many of 
      the features characteristic of RNA tumor viruses. In addition to the expected 
      size (600 S) and density (1.16 g/ml) these include possession of an outer 
      membrane and an inner one surrounding a "core" containing a DNA polymerase and a 
      large-molecular-weight (70S) RNA possessing detectable homology to the RNAs of 
      the mouse mammary tumor virus (MMTV) and of the Mason-Pfizer monkey virus (MPMV). 
      We report here the purification and characterization of the DNA polymerase from 
      the human breast cancer particles. Its key properties are very similar to those 
      ofthe RNA-dependent DNA nucleotidyltransferase (reverse transcriptase) found in 
      MMTV and MPMV. Thus like these viral enzymes, the purified human breast cancer 
      DNA polymerase exhibits the following three features that together distinguish 
      the known viral reverse transcriptases from normal cellular DNA polymerases: (i) 
      a strong preference for oligo(dT)-poly(rA) over oligo(dT)-poly(dA) as a template 
      for the synthesis of poly(dT); (ii) the acceptance of the highly specific 
      oligo(dG)-poly(rCm) as a template for the formation of poly(dG); (iii) the 
      ability to use a viral RNA (AMV) as a template to fashion a faithful DNA 
      complementary copy; and (iv) its preference for Mg++ over Mn++. In summary, the 
      data described here on the enzyme of the human breast cancer particles add 
      further evidence of similarities to the viral agents associated with the 
      corresponding malignancies in the mouse and monkey models. To date, an enzyme 
      with these properties has not been detected in normal breast tissues or in benign 
      tumors of the breast.
FAU - Ohno, T
AU  - Ohno T
FAU - Sweet, R W
AU  - Sweet RW
FAU - Dejak, D
AU  - Dejak D
FAU - Spiegelman, S
AU  - Spiegelman S
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA, Neoplasm)
RN  - 42Z2K6ZL8P (Manganese)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
RN  - I38ZP9992A (Magnesium)
SB  - IM
MH  - Breast Neoplasms/*enzymology
MH  - DNA, Neoplasm
MH  - *DNA-Directed DNA Polymerase/isolation & purification/metabolism
MH  - Enzyme Activation
MH  - Female
MH  - Humans
MH  - Kinetics
MH  - Magnesium/pharmacology
MH  - Manganese/pharmacology
MH  - Nucleic Acid Hybridization
MH  - Subcellular Fractions/enzymology
MH  - Templates, Genetic
PMC - PMC392374
EDAT- 1977/02/01 00:00
MHDA- 1977/02/01 00:01
PMCR- 1977/08/01
CRDT- 1977/02/01 00:00
PHST- 1977/02/01 00:00 [pubmed]
PHST- 1977/02/01 00:01 [medline]
PHST- 1977/02/01 00:00 [entrez]
PHST- 1977/08/01 00:00 [pmc-release]
AID - 10.1073/pnas.74.2.764 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1977 Feb;74(2):764-8. doi: 10.1073/pnas.74.2.764.