PMID- 26554788
OWN - NLM
STAT- MEDLINE
DCOM- 20160218
LR  - 20181113
IS  - 1536-5964 (Electronic)
IS  - 0025-7974 (Print)
IS  - 0025-7974 (Linking)
VI  - 94
IP  - 44
DP  - 2015 Nov
TI  - Immunologic Monitoring of T-Lymphocyte Subsets and Hla-Dr-Positive Monocytes in 
      Kidney Transplant Recipients: A Prospective, Observational Cohort Study.
PG  - e1902
LID - 10.1097/MD.0000000000001902 [doi]
LID - e1902
AB  - The clinical significance of circulating T-lymphocyte subsets and human leukocyte 
      antigen (HLA)-DR-positive monocytes in the peripheral blood of kidney transplant 
      recipients (KTRs) remains unclear. We examined the efficacy of enumerating these 
      cells for the immunologic monitoring of KTRs.Blood samples were obtained before 
      transplantation, 2 weeks after transplantation and at diagnosis, and 2 weeks 
      after treating biopsy-proven acute cellular rejection and cytomegalovirus (CMV) 
      infection. Serial flow cytometric analysis was performed using peripheral blood 
      obtained from 123 patients to identify the frequencies of HLA-DR, CD3, CD4, CD8, 
      and CD25 T-lymphocytes and HLA-DR-positive monocytes.Frequencies of CD4CD25/CD4 T 
      cells, CD8CD25/CD8 T cells, and HLA-DR-positive monocytes were significantly 
      lower at 2 weeks after transplantation than before transplantation (all 
      P < 0.001). This decrease was not correlated with clinical parameters. The 
      frequency of CD4CD25/CD4 T cells was significantly higher in KTRs with acute 
      rejection than in KTRs at 2 weeks after transplantation (9.10% [range 4.30-25.6%] 
      vs 5.10% [range 0.10-33.3%]; P = 0.024). However, no significant differences were 
      observed between stable KTRs and KTRs with CMV infection. Analysis of the 
      receiver operating characteristic curve adjusted by covariates showed that acute 
      rejection could be predicted with 75.0% sensitivity and 68.4% specificity by 
      setting the cutoff value of CD4CD25/CD4 T cell frequency as 5.8%.Circulating 
      T-lymphocyte and monocyte subsets showed significant and consistent changes in 
      their frequencies after immunosuppression. Of the various immune cells examined, 
      circulating levels of CD4CD25 T cells might be a useful noninvasive immunologic 
      indicator for detecting acute rejection.
FAU - Cho, Jang-Hee
AU  - Cho JH
AD  - From the Department of Internal Medicine (J-HC, Y-DY, EK, H-YJ, J-YC, S-HP, Y-LK, 
      C-DK); Department of Statistics (HMJ); Department of Surgery (H-KK, SH); and 
      Department of Clinical Pathology, Kyungpook National University Hospital, Daegu, 
      Korea (D-IW).
FAU - Yoon, Young-Deuk
AU  - Yoon YD
FAU - Jang, Hye Min
AU  - Jang HM
FAU - Kwon, Eugene
AU  - Kwon E
FAU - Jung, Hee-Yeon
AU  - Jung HY
FAU - Choi, Ji-Young
AU  - Choi JY
FAU - Park, Sun-Hee
AU  - Park SH
FAU - Kim, Yong-Lim
AU  - Kim YL
FAU - Kim, Hyung-Kee
AU  - Kim HK
FAU - Huh, Seung
AU  - Huh S
FAU - Won, Dong-Il
AU  - Won DI
FAU - Kim, Chan-Duck
AU  - Kim CD
LA  - eng
PT  - Journal Article
PT  - Observational Study
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Medicine (Baltimore)
JT  - Medicine
JID - 2985248R
RN  - 0 (HLA-DR Antigens)
SB  - IM
EIN - Medicine (Baltimore). 2016 Jan 22;95(3):e8921. PMID: 31265609
MH  - Adult
MH  - Aged
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Female
MH  - Flow Cytometry
MH  - Follow-Up Studies
MH  - Graft Rejection/*immunology/pathology
MH  - HLA-DR Antigens/*immunology
MH  - Humans
MH  - *Immunity, Cellular
MH  - *Kidney Transplantation
MH  - Lymphocyte Activation
MH  - Lymphocyte Count
MH  - Male
MH  - Middle Aged
MH  - Monitoring, Immunologic/*methods
MH  - Prospective Studies
MH  - T-Lymphocyte Subsets/*immunology
MH  - Young Adult
PMC - PMC4915889
COIS- The authors declare that they do not have any competing interests.
EDAT- 2015/11/12 06:00
MHDA- 2016/02/19 06:00
PMCR- 2015/11/06
CRDT- 2015/11/12 06:00
PHST- 2015/11/12 06:00 [entrez]
PHST- 2015/11/12 06:00 [pubmed]
PHST- 2016/02/19 06:00 [medline]
PHST- 2015/11/06 00:00 [pmc-release]
AID - 00005792-201511030-00028 [pii]
AID - 10.1097/MD.0000000000001902 [doi]
PST - ppublish
SO  - Medicine (Baltimore). 2015 Nov;94(44):e1902. doi: 10.1097/MD.0000000000001902.