PMID- 26555162
OWN - NLM
STAT- MEDLINE
DCOM- 20161111
LR  - 20170812
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 213
DP  - 2016 Feb 2
TI  - Evaluation of the use of non-pathogenic porcine circovirus type 1 as a vaccine 
      delivery virus vector to express antigenic epitopes of porcine reproductive and 
      respiratory syndrome virus.
PG  - 100-108
LID - S0168-1702(15)30112-X [pii]
LID - 10.1016/j.virusres.2015.11.005 [doi]
AB  - We previously demonstrated that the C-terminus of the capsid gene of porcine 
      circovirus type 2 (PCV2) is an immune reactive epitope displayed on the surface 
      of virions. Insertion of foreign epitope tags in the C-terminus produced 
      infectious virions that elicited humoral immune responses against both PCV2 
      capsid and the inserted epitope tags, whereas mutation in the N terminus impaired 
      viral replication. Since the non-pathogenic porcine circovirus type 1 (PCV1) 
      shares similar genomic organization and significant sequence identity with 
      pathogenic PCV2, in this study we evaluated whether PCV1 can serve as a vaccine 
      delivery virus vector. Four different antigenic determinants of porcine 
      reproductive and respiratory syndrome virus (PRRSV) were inserted in the 
      C-terminus of the PCV1 capsid gene, the infectivity and immunogenicity of the 
      resulting viruses are determined. We showed that an insertion of 12 (PRRSV-GP2 
      epitope II, PRRSV-GP3 epitope I, and PRRSV-GP5 epitope I), and 14 (PRRSV-GP5 
      epitope IV) amino acid residues did not affect PCV1 replication. We successfully 
      rescued and characterized four chimeric PCV1 viruses expressing PRRSV linear 
      antigenic determinants (GP2 epitope II: aa 40-51, ASPSHVGWWSFA; GP3 epitope I: aa 
      61-72, QAAAEAYEPGRS; GP5 epitope I: aa 35-46, SSSNLQLIYNLT; and GP5 epitope IV: 
      aa 187-200, TPVTRVSAEQWGRP). We demonstrated that all chimeric viruses were 
      stable and infectious in vitro and three chimeric viruses were infectious in 
      vivo. An immunogenicity study in pigs revealed that PCV1-VR2385EPI chimeric 
      viruses elicited neutralizing antibodies against PRRSV-VR2385. The results have 
      important implications for further evaluating PCV1 as a potential vaccine 
      delivery vector.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Pineyro, Pablo E
AU  - Pineyro PE
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA 24060, USA; Department of Veterinary Diagnostic and Production 
      Animal Medicine, Iowa State University College of Veterinary Medicine, Ames, IA 
      5001, USA.
FAU - Kenney, Scott P
AU  - Kenney SP
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA 24060, USA.
FAU - Gimenez-Lirola, Luis G
AU  - Gimenez-Lirola LG
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State 
      University College of Veterinary Medicine, Ames, IA 5001, USA.
FAU - Opriessnig, Tanja
AU  - Opriessnig T
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State 
      University College of Veterinary Medicine, Ames, IA 5001, USA; The Roslin 
      Institute and The Royal (Dick) School of Veterinary Studies, University of 
      Edinburgh, Edinburgh, United Kingdom.
FAU - Tian, Debin
AU  - Tian D
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA 24060, USA.
FAU - Heffron, C Lynn
AU  - Heffron CL
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA 24060, USA.
FAU - Meng, Xiang-Jin
AU  - Meng XJ
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA 24060, USA. Electronic address: xjmeng@vt.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151107
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Drug Carriers)
RN  - 0 (Epitopes)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/blood
MH  - Antibodies, Viral/blood
MH  - Antigens, Viral/genetics/*immunology
MH  - Circovirus/*genetics/physiology
MH  - *Drug Carriers
MH  - Epitopes/genetics/*immunology
MH  - Genetic Vectors
MH  - Genomic Instability
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Swine
MH  - Vaccines, Synthetic/genetics/immunology
MH  - Viral Vaccines/*genetics/*immunology
MH  - Virus Replication
OTO - NOTNLM
OT  - Antigenic epitopes
OT  - Porcine circovirus type 1 (PCV1)
OT  - Porcine circovirus type 2 (PCV2)
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Vaccine delivery vector
EDAT- 2015/11/12 06:00
MHDA- 2016/11/12 06:00
CRDT- 2015/11/12 06:00
PHST- 2015/08/22 00:00 [received]
PHST- 2015/11/02 00:00 [revised]
PHST- 2015/11/04 00:00 [accepted]
PHST- 2015/11/12 06:00 [entrez]
PHST- 2015/11/12 06:00 [pubmed]
PHST- 2016/11/12 06:00 [medline]
AID - S0168-1702(15)30112-X [pii]
AID - 10.1016/j.virusres.2015.11.005 [doi]
PST - ppublish
SO  - Virus Res. 2016 Feb 2;213:100-108. doi: 10.1016/j.virusres.2015.11.005. Epub 2015 
      Nov 7.