PMID- 26555398
OWN - NLM
STAT- MEDLINE
DCOM- 20161017
LR  - 20220318
IS  - 1573-7365 (Electronic)
IS  - 0885-7490 (Linking)
VI  - 31
IP  - 1
DP  - 2016 Feb
TI  - Behavioural and biochemical changes in maternally separated Sprague-Dawley rats 
      exposed to restraint stress.
PG  - 121-33
LID - 10.1007/s11011-015-9757-y [doi]
AB  - Early life adversity has been associated with the development of various 
      neuropsychiatric disorders in adulthood such as depression and anxiety. The aim 
      of this study was to determine if stress during adulthood can exaggerate the 
      depression-/anxiety-like behaviour observed in the widely accepted maternally 
      separated (MS) Sprague-Dawley (SD) rat model of depression. A further aim was to 
      determine whether the behavioural changes were accompanied by changes in 
      hippocampal brain-derived neurotrophic factor (BDNF) and the protein profile of 
      the prefrontal cortex (PFC). Depression-/anxiety-like behaviour was measured in 
      the elevated plus maze, open field and forced swim test (FST) in the MS SD rats 
      exposed to chronic restraint stress in adulthood. As expected, MS increased 
      immobility of SD rats in the FST but restraint stress did not enhance this effect 
      of MS on SD rats. A proteomic analysis of the PFC revealed a decrease in 
      actin-related proteins in MS and non-separated rats subjected to restraint stress 
      as well as a decrease in mitochondrial energy-related proteins in the stressed 
      rat groups. Since MS during early development causes a disruption in the 
      hypothalamic-pituitary-adrenal axis and long-term changes in the response to 
      subsequent stress, it may have prevented restraint stress from exerting its 
      effects on behaviour. Moreover, the decrease in proteins related to mitochondrial 
      energy metabolism in MS rats with or without subsequent restraint stress may be 
      related to stress per se and not depression-like behaviour, because rats 
      subjected to restraint stress displayed similar decreases in energy-related 
      proteins and spent less time immobile in the FST than control rats.
FAU - van Zyl, P J
AU  - van Zyl PJ
AD  - Department of Human Biology, Faculty of Health Sciences, University of Cape Town, 
      Observatory, 7925, South Africa. vanzyl.jurgens@gmail.com.
FAU - Dimatelis, J J
AU  - Dimatelis JJ
AD  - Department of Human Biology, Faculty of Health Sciences, University of Cape Town, 
      Observatory, 7925, South Africa.
FAU - Russell, V A
AU  - Russell VA
AD  - Department of Human Biology, Faculty of Health Sciences, University of Cape Town, 
      Observatory, 7925, South Africa.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151111
PL  - United States
TA  - Metab Brain Dis
JT  - Metabolic brain disease
JID - 8610370
RN  - 0 (Actins)
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Anxiety, Separation/*metabolism/*psychology
MH  - *Behavior, Animal
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Energy Metabolism
MH  - Female
MH  - Male
MH  - *Maternal Deprivation
MH  - Mitochondria/metabolism
MH  - Prefrontal Cortex/metabolism
MH  - Proteomics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Restraint, Physical
MH  - Stress, Psychological/*metabolism/*psychology
OTO - NOTNLM
OT  - Behaviour
OT  - Depression
OT  - Maternal separation
OT  - Proteomics
OT  - Restraint stress
EDAT- 2015/11/12 06:00
MHDA- 2016/10/19 06:00
CRDT- 2015/11/12 06:00
PHST- 2015/06/30 00:00 [received]
PHST- 2015/10/27 00:00 [accepted]
PHST- 2015/11/12 06:00 [entrez]
PHST- 2015/11/12 06:00 [pubmed]
PHST- 2016/10/19 06:00 [medline]
AID - 10.1007/s11011-015-9757-y [pii]
AID - 10.1007/s11011-015-9757-y [doi]
PST - ppublish
SO  - Metab Brain Dis. 2016 Feb;31(1):121-33. doi: 10.1007/s11011-015-9757-y. Epub 2015 
      Nov 11.