PMID- 26556105
OWN - NLM
STAT- MEDLINE
DCOM- 20160914
LR  - 20151130
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 77
DP  - 2016 Jan
TI  - Majoon ushba, a polyherbal compound ameliorates rheumatoid arthritis via 
      regulating inflammatory and bone remodeling markers in rats.
PG  - 115-26
LID - S1043-4666(15)30090-9 [pii]
LID - 10.1016/j.cyto.2015.11.002 [doi]
AB  - The present study was aimed to investigate the anti-arthritic effect of majoon 
      ushba (MU) and its underlying mechanism in adjuvant induced arthritis (AIA) rats. 
      Arthritis was induced by intradermal injection of complete freund's adjuvant 
      (0.1ml) into the right hind paw of the Wistar albino rats. MU (1000mg/kg/b.wt) 
      and methotrexate (3mg/kg/b.wt) were administered from day 11 to day 18th for 
      8days after adjuvant induction. We have found that MU treatment significantly 
      increased the level of anti-inflammatory cytokine (IL-10) and inhibited the over 
      production of pro-inflammatory cytokines (TNF-alpha, IL-1beta, and IL-6) and monocyte 
      chemoattractant protein-1 (MCP-1) (ELISA) in the serum of adjuvant-induced 
      arthritic rats. The mRNA expression of pro-inflammatory cytokines (TNF-alpha, IL-1beta, 
      IL-6, and IL-17), inflammatory enzymes (inducible nitric oxide synthase (iNOS) 
      and cyclo-oxygenase-2 (COX-2)), MCP-1, receptor activator of nuclear factor-kB 
      ligand (RANKL) and transcription factors (NF-small ka, CyrillicB and AP-1) (Real-Time PCR) was 
      found significantly downregulated in the synovial tissues of MU treated arthritic 
      rats. In addition, the protein expression of NF-small ka, CyrillicB, IL-17, COX-2, and RANKL 
      (western blotting and immunohistochemistry analysis) was found reduced. On the 
      other hand, osteoprotegerin (OPG), a bone remodeling marker was found to be 
      elevated in synovial tissues of MU treated arthritic rats. Furthermore, MU 
      treatment prevented body weight loss and reduced the joint paw edema, cell 
      infiltration, cartilage and bone degradation as evidenced by the 
      histopathological and radiological analysis. In conclusion, our current findings 
      provide scientific evidence for the traditional claim of MU as an anti-arthritic 
      drug.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Ganesan, Ramamoorthi
AU  - Ganesan R
AD  - Immunopathology Lab, School of Bio Sciences and Technology, VIT University, 
      Vellore 632 014, Tamil Nadu, India.
FAU - Doss, Hari Madhuri
AU  - Doss HM
AD  - Immunopathology Lab, School of Bio Sciences and Technology, VIT University, 
      Vellore 632 014, Tamil Nadu, India.
FAU - Rasool, Mahaboobkhan
AU  - Rasool M
AD  - Immunopathology Lab, School of Bio Sciences and Technology, VIT University, 
      Vellore 632 014, Tamil Nadu, India. Electronic address: mkr474@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151108
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Preparations)
RN  - 0 (majoon ushba)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Animals
MH  - Arthritis, Experimental/genetics/metabolism/*prevention & control
MH  - Arthritis, Rheumatoid/genetics/metabolism/*prevention & control
MH  - Biomarkers/blood/*metabolism
MH  - Blotting, Western
MH  - Bone Remodeling/*drug effects
MH  - Chemokine CCL2/genetics/metabolism
MH  - Cyclooxygenase 2/genetics/metabolism
MH  - Cytokines/blood/genetics/metabolism
MH  - Edema/prevention & control
MH  - Female
MH  - Gene Expression/drug effects
MH  - Hindlimb/drug effects/metabolism/pathology
MH  - Immunohistochemistry
MH  - Inflammation Mediators/blood/*metabolism
MH  - Male
MH  - NF-kappa B/genetics/metabolism
MH  - Nitric Oxide Synthase Type II/genetics/metabolism
MH  - Phytotherapy/methods
MH  - Plant Preparations/*pharmacology
MH  - Plants, Medicinal/chemistry
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
OTO - NOTNLM
OT  - Inflammatory enzymes
OT  - Majoon ushba
OT  - Pro-inflammatory cytokines
OT  - Rheumatoid arthritis
EDAT- 2015/11/12 06:00
MHDA- 2016/09/15 06:00
CRDT- 2015/11/12 06:00
PHST- 2015/07/03 00:00 [received]
PHST- 2015/10/20 00:00 [revised]
PHST- 2015/11/02 00:00 [accepted]
PHST- 2015/11/12 06:00 [entrez]
PHST- 2015/11/12 06:00 [pubmed]
PHST- 2016/09/15 06:00 [medline]
AID - S1043-4666(15)30090-9 [pii]
AID - 10.1016/j.cyto.2015.11.002 [doi]
PST - ppublish
SO  - Cytokine. 2016 Jan;77:115-26. doi: 10.1016/j.cyto.2015.11.002. Epub 2015 Nov 8.