PMID- 26556288
OWN - NLM
STAT- MEDLINE
DCOM- 20160926
LR  - 20151216
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 7
IP  - 49
DP  - 2015 Dec 16
TI  - Bioinert Anodic Alumina Nanotubes for Targeting of Endoplasmic Reticulum Stress 
      and Autophagic Signaling: A Combinatorial Nanotube-Based Drug Delivery System for 
      Enhancing Cancer Therapy.
PG  - 27140-51
LID - 10.1021/acsami.5b07557 [doi]
AB  - Although nanoparticle-based targeted delivery systems have gained promising 
      achievements for cancer therapy, the development of sophisticated strategies with 
      effective combinatorial therapies remains an enduring challenge. Herein, we 
      report the fabrication of a novel nanomaterial, so-called anodic alumina 
      nanotubes (AANTs) for proof-of-concept cancer therapy by targeting cell signaling 
      networks. This strategy is to target autophagic and endoplasmic reticulum (ER) 
      stress signaling by using thapsigargin (TG)-loaded AANTs cotreated with an 
      autophagy inhibitor 3-methyladenine (3-MA). We first show that AANTs are nontoxic 
      and can activate autophagy in different cell types including human fibroblast 
      cells (HFF), human monocyte cells (THP-1), and human breast cancer cells (MDA-MB 
      231-TXSA). Treatment with 3-MA at a nontoxic dose reduced the level of autophagy 
      induced by AANTs, and consequently sensitized breast cancer cells to 
      AANTs-induced cellular stresses. To target autophagic and ER stress signaling 
      networking, breast cancer cells were treated with 3-MA together with AANTs loaded 
      with the prototype ER stress inducer TG. We demonstrated that 3-MA enhanced the 
      cancer cell killing effect of AANTs loaded with TG. This effect was associated 
      with enhanced ER stress signaling due to the combination effect of TG and 3-MA. 
      These findings not only demonstrate the excellent biocompatibility of AANTs as 
      novel biomaterials but also provide new opportunities for developing ER- and 
      autophagy-targeted delivery systems for future clinical cancer therapy.
FAU - Wang, Ye
AU  - Wang Y
AD  - School of Chemical Engineering and double daggerDiscipline of Surgery, Basil Hetzel 
      Institute, The University of Adelaide , Adelaide, South Australia 5005, 
      Australia.
FAU - Kaur, Gagandeep
AU  - Kaur G
AD  - School of Chemical Engineering and double daggerDiscipline of Surgery, Basil Hetzel 
      Institute, The University of Adelaide , Adelaide, South Australia 5005, 
      Australia.
FAU - Chen, Yuting
AU  - Chen Y
AD  - School of Chemical Engineering and double daggerDiscipline of Surgery, Basil Hetzel 
      Institute, The University of Adelaide , Adelaide, South Australia 5005, 
      Australia.
FAU - Santos, Abel
AU  - Santos A
AD  - School of Chemical Engineering and double daggerDiscipline of Surgery, Basil Hetzel 
      Institute, The University of Adelaide , Adelaide, South Australia 5005, 
      Australia.
FAU - Losic, Dusan
AU  - Losic D
AD  - School of Chemical Engineering and double daggerDiscipline of Surgery, Basil Hetzel 
      Institute, The University of Adelaide , Adelaide, South Australia 5005, 
      Australia.
FAU - Evdokiou, Andreas
AU  - Evdokiou A
AD  - School of Chemical Engineering and double daggerDiscipline of Surgery, Basil Hetzel 
      Institute, The University of Adelaide , Adelaide, South Australia 5005, 
      Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151201
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 333DO1RDJY (Serotonin)
RN  - 7440-57-5 (Gold)
RN  - LMI26O6933 (Aluminum Oxide)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Aluminum Oxide/*chemistry
MH  - Animals
MH  - Autophagy
MH  - Catalysis
MH  - Dopamine/metabolism
MH  - Endoplasmic Reticulum Stress/physiology
MH  - Gold/*chemistry
MH  - Humans
MH  - Metal Nanoparticles/*chemistry
MH  - Nanotubes/*chemistry
MH  - Serotonin/metabolism
MH  - Signal Transduction/physiology
OTO - NOTNLM
OT  - anodic alumina nanotubes
OT  - autophagy
OT  - cell signaling
OT  - drug delivery
OT  - endoplasmic reticulum stress
OT  - nanotoxicity
EDAT- 2015/11/12 06:00
MHDA- 2016/09/27 06:00
CRDT- 2015/11/12 06:00
PHST- 2015/11/12 06:00 [entrez]
PHST- 2015/11/12 06:00 [pubmed]
PHST- 2016/09/27 06:00 [medline]
AID - 10.1021/acsami.5b07557 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2015 Dec 16;7(49):27140-51. doi: 
      10.1021/acsami.5b07557. Epub 2015 Dec 1.