PMID- 26557913
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151111
LR  - 20181113
IS  - 1759-7706 (Print)
IS  - 1759-7714 (Electronic)
IS  - 1759-7706 (Linking)
VI  - 6
IP  - 6
DP  - 2015 Nov
TI  - Loss of heterozygosity at the human leukocyte antigen locus in thymic epithelial 
      tumors.
PG  - 749-53
LID - 10.1111/1759-7714.12252 [doi]
AB  - BACKGROUND: To study the relationship between loss of heterozygosity (LOH) at the 
      human leukocyte antigen (HLA) locus and the pathogenicity and clinicopathological 
      features of thymic epithelial tumors (TET). METHODS: Tumor and adjacent normal 
      tissues were isolated from 36 TET patients. Five microsatellite loci (D6S1666, 
      D6S265, D6S273, DS6276, and D6S291) within the HLA locus were amplified by 
      polymerase chain reaction. DNA sequencing was used to measure the frequency of 
      microsatellite LOH. RESULTS: LOH was identified in at least one locus in 83.6% of 
      TET patients. LOH frequency at D6S1666, D6S265, D6S273, D6S276, and D6S291 was 
      44.4%, 16.7%, 30.5%, 38.9%, and 36.1% respectively. There was no significant 
      association between LOH frequency in TET with tumor severity, or in the presence 
      or absence of myasthenia gravis. CONCLUSIONS: D6S1666, D6S265, D6S273, DS6S276, 
      and D6S29 are sensitive loci for studying microsatellite LOH in TET. LOH within 
      the HLA complex is implicated in the occurrence and development of TET, with the 
      HLA-DQA1 gene likely involved. However, an understanding of the relationship 
      between LOH and the clinicopathological features of TET requires a larger sample 
      size than that of the present study.
FAU - Chen, Yuan
AU  - Chen Y
AD  - Deparment of Cardiothoracic Surgery, Tianjin Medical University General Hospital 
      Tianjin, China.
FAU - Wang, Guojin
AU  - Wang G
AD  - Deparment of Hematology and Oncology, Tianjin Medical University General Hospital 
      Tianjin, China.
FAU - Zhang, Peng
AU  - Zhang P
AD  - Deparment of Cardiothoracic Surgery, Tianjin Medical University General Hospital 
      Tianjin, China.
FAU - Liu, Yimei
AU  - Liu Y
AD  - Deparment of Cardiothoracic Surgery, Tianjin Medical University General Hospital 
      Tianjin, China.
FAU - Yao, Yuanyuan
AU  - Yao Y
AD  - Deparment of Cardiothoracic Surgery, Tianjin Medical University General Hospital 
      Tianjin, China.
FAU - Wang, Hai
AU  - Wang H
AD  - Deparment of Cardiothoracic Surgery, Tianjin Medical University General Hospital 
      Tianjin, China.
FAU - Wang, Yuanguo
AU  - Wang Y
AD  - Deparment of Cardiothoracic Surgery, Tianjin Medical University General Hospital 
      Tianjin, China.
LA  - eng
PT  - Journal Article
DEP - 20150409
PL  - Singapore
TA  - Thorac Cancer
JT  - Thoracic cancer
JID - 101531441
PMC - PMC4632927
OTO - NOTNLM
OT  - Human leukocyte antigen (HLA)
OT  - loss of heterozygosity (LOH)
OT  - microsatellite
OT  - thymic epithelial tumor (TET)
EDAT- 2015/11/12 06:00
MHDA- 2015/11/12 06:01
PMCR- 2015/11/01
CRDT- 2015/11/12 06:00
PHST- 2014/11/08 00:00 [received]
PHST- 2015/01/05 00:00 [accepted]
PHST- 2015/11/12 06:00 [entrez]
PHST- 2015/11/12 06:00 [pubmed]
PHST- 2015/11/12 06:01 [medline]
PHST- 2015/11/01 00:00 [pmc-release]
AID - 10.1111/1759-7714.12252 [doi]
PST - ppublish
SO  - Thorac Cancer. 2015 Nov;6(6):749-53. doi: 10.1111/1759-7714.12252. Epub 2015 Apr 
      9.