PMID- 265582
OWN - NLM
STAT- MEDLINE
DCOM- 19770512
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 74
IP  - 3
DP  - 1977 Mar
TI  - Molecular weight and valence of the cell-surface receptor for immunoglobulin E.
PG  - 869-72
AB  - The molecular weight of the active solubilized cell-surface receptor for 
      immunoglobulin E (IgE) was measured in nonionic detergent. The diffusion 
      coefficient was estimated by gel filtration, the partial specific volume was 
      estimated from the differential sedimentation in sucrose gradients prepared from 
      H2O and D2O, and the sedimentation constant was estimated from the same 
      centrifugation experiments. The receptor has an apparent molecular weight of 
      130,000. Its high partial specific volume (0.81 cm3/g) suggests that bound 
      detergent contributes significantly to the mass. The molecular weights of the 
      receptor-IgE complex and of unbound IgE determined similarly were 310,000 and 
      200,000 respectively, clearly showing that the receptor is univalent. The 
      implications of these results for the subunit structure of the receptor, 
      receptor-membrane integration, and a possible mechanism of receptor triggering 
      are discussed.
FAU - Newman, S A
AU  - Newman SA
FAU - Rossi, G
AU  - Rossi G
FAU - Metzger, H
AU  - Metzger H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Detergents)
RN  - 0 (Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Basophils/immunology
MH  - *Binding Sites
MH  - Detergents
MH  - Diffusion
MH  - Glycoproteins/metabolism
MH  - Immunoglobulin E/*metabolism
MH  - *Membrane Proteins/metabolism
MH  - Models, Structural
MH  - Molecular Weight
MH  - Protein Conformation
MH  - Rats
MH  - Solubility
MH  - Ultracentrifugation
PMC - PMC430509
EDAT- 1977/03/01 00:00
MHDA- 1977/03/01 00:01
PMCR- 1977/09/01
CRDT- 1977/03/01 00:00
PHST- 1977/03/01 00:00 [pubmed]
PHST- 1977/03/01 00:01 [medline]
PHST- 1977/03/01 00:00 [entrez]
PHST- 1977/09/01 00:00 [pmc-release]
AID - 10.1073/pnas.74.3.869 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1977 Mar;74(3):869-72. doi: 10.1073/pnas.74.3.869.