PMID- 26560124
OWN - NLM
STAT- MEDLINE
DCOM- 20160819
LR  - 20191210
IS  - 1471-2407 (Electronic)
IS  - 1471-2407 (Linking)
VI  - 15
DP  - 2015 Nov 11
TI  - CIP2A overexpression induces autoimmune response and enhances JNK signaling 
      pathway in human lung cancer.
PG  - 895
LID - 10.1186/s12885-015-1899-0 [doi]
LID - 895
AB  - BACKGROUND: Cancerous inhibitor of PP2A (CIP2A) is a recently characterized 
      oncoprotein, which promotes cancer cell proliferation. But the role of CIP2A in 
      lung cancer progression is still not well understood. METHODS: The expression 
      level of CIP2A in lung cancer tissues was examined by immunohistochemistry. 
      CIP2A-associated cell proliferation was performed by knock down or overexpression 
      of CIP2A in lung cancer cells. Phospho-array was used to screen kinase candidates 
      related to expression change of CIP2A. Western-blot and luciferase reporter assay 
      were used to validate phospho-array results. RESULTS: Overexpression of CIP2A in 
      lung cancer not only triggers immune response in lung cancer patients but also 
      promotes lung cancer cell proliferation. By phospho-array, several kinase 
      candidates were identified, one of which is c-Jun activated kinases (JNK). The 
      knock down of CIP2A decreased JNK phosphorylation, and the phosphorylation of 
      downstream transcriptional factors, ATF2 and c-Jun, whose transcriptional 
      activity were decreased as well. Furthermore, the expression level of CIP2A also 
      affected the phosphorylation of the upstream kinase of JNK, MKK4/MKK7. At last, 
      treatment with JNK inhibitor partially abolished CIP2A-induced cell 
      proliferation. CONCLUSION: CIP2A is a tumor-associated autoantigen in lung 
      cancer, which promote lung cancer proliferation partially through MKK4/7-JNK 
      signaling pathway.
FAU - Peng, Bo
AU  - Peng B
AD  - Border Biomedical Research Center & Department of Biological Sciences, The 
      University of Texas at El Paso, El Paso, TX, 79968, USA. bpeng2@miners.utep.edu.
FAU - Chai, Yurong
AU  - Chai Y
AD  - Border Biomedical Research Center & Department of Biological Sciences, The 
      University of Texas at El Paso, El Paso, TX, 79968, USA. yrchai@live.cn.
FAU - Li, Yang
AU  - Li Y
AD  - Border Biomedical Research Center & Department of Biological Sciences, The 
      University of Texas at El Paso, El Paso, TX, 79968, USA. yli5@miners.utep.edu.
FAU - Liu, Xinxin
AU  - Liu X
AD  - Border Biomedical Research Center & Department of Biological Sciences, The 
      University of Texas at El Paso, El Paso, TX, 79968, USA. xliu2@miners.utep.edu.
FAU - Zhang, Jianying
AU  - Zhang J
AD  - Border Biomedical Research Center & Department of Biological Sciences, The 
      University of Texas at El Paso, El Paso, TX, 79968, USA. jzhang@utep.edu.
LA  - eng
GR  - G12 MD007592/MD/NIMHD NIH HHS/United States
GR  - SC1 CA166016/CA/NCI NIH HHS/United States
GR  - 5G12MD007592/MD/NIMHD NIH HHS/United States
GR  - SC1CA166016/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20151111
PL  - England
TA  - BMC Cancer
JT  - BMC cancer
JID - 100967800
RN  - 0 (Autoantibodies)
RN  - 0 (Autoantigens)
RN  - 0 (CIP2A protein, human)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 7)
SB  - IM
MH  - Autoantibodies/blood/immunology
MH  - Autoantigens/*genetics/immunology/metabolism
MH  - Autoimmunity/*genetics
MH  - Case-Control Studies
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - *Gene Expression
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - JNK Mitogen-Activated Protein Kinases/*metabolism
MH  - Lung Neoplasms/*etiology/*metabolism
MH  - MAP Kinase Kinase 4/metabolism
MH  - MAP Kinase Kinase 7/metabolism
MH  - Membrane Proteins/*genetics/immunology/metabolism
MH  - Proto-Oncogene Proteins c-jun/metabolism
MH  - *Signal Transduction
PMC - PMC4642650
EDAT- 2015/11/13 06:00
MHDA- 2016/08/20 06:00
PMCR- 2015/11/11
CRDT- 2015/11/13 06:00
PHST- 2014/10/03 00:00 [received]
PHST- 2015/11/03 00:00 [accepted]
PHST- 2015/11/13 06:00 [entrez]
PHST- 2015/11/13 06:00 [pubmed]
PHST- 2016/08/20 06:00 [medline]
PHST- 2015/11/11 00:00 [pmc-release]
AID - 10.1186/s12885-015-1899-0 [pii]
AID - 1899 [pii]
AID - 10.1186/s12885-015-1899-0 [doi]
PST - epublish
SO  - BMC Cancer. 2015 Nov 11;15:895. doi: 10.1186/s12885-015-1899-0.