PMID- 2656018
OWN - NLM
STAT- MEDLINE
DCOM- 19890710
LR  - 20190820
IS  - 0090-1229 (Print)
IS  - 0090-1229 (Linking)
VI  - 52
IP  - 1
DP  - 1989 Jul
TI  - Immunophenotyping in a multicenter study: the Transfusion Safety Study 
      experience.
PG  - 38-47
AB  - The Transfusion Safety Study (TSS) is a cooperative investigation of factors that 
      determine the occurrence of and modify the expression of transfusion-transmitted 
      infections. A major component of its data is derived from lymphocyte 
      immunophenotyping using a large panel of monoclonal antibodies and two-color flow 
      cytometric analysis. The multicenter longitudinal character of TSS necessitates a 
      uniformity of instrumentation, reagents, and protocols, as well as an intensive 
      quality control program. The baseline assessment of a cohort of males 10 years of 
      age and over with congenital clotting disorders (CCD) exemplifies the approach 
      and some of the flow cytometry results. A comparison of anti-HIV-1 positive and 
      negative subjects shows that more of the loss of T4+ cells was attributable to a 
      decrease in the T4+4B4+ subset than the T4+2H4+ subset. There was an overall 
      increase in CD8 cells, with a significant increase in the I2+T8+ and Leu7+T8+ 
      cells, but a fall in NKH.1+T8+ cells. Monocytes, MO2+I2+ cells, increased. In CCD 
      patients under the age of 10, both anti-HIV-1 positive and negative, there were 
      absolute elevations in immunocytes, including CD4. There was also a distinctly 
      different distribution of CD4 subsets. The suppressor inducer subset, 2H4+T4+, 
      was increased relative to the helper inducer subset, 4B4+T4+, in the younger 
      subjects.
FAU - Fletcher, M A
AU  - Fletcher MA
AD  - University of Miami School of Medicine, Florida 33101.
FAU - Azen, S P
AU  - Azen SP
FAU - Adelsberg, B
AU  - Adelsberg B
FAU - Gjerset, G
AU  - Gjerset G
FAU - Hassett, J
AU  - Hassett J
FAU - Kaplan, J
AU  - Kaplan J
FAU - Niland, J C
AU  - Niland JC
FAU - Odom-Maryon, T
AU  - Odom-Maryon T
FAU - Parker, J W
AU  - Parker JW
FAU - Stites, D P
AU  - Stites DP
AU  - et al.
LA  - eng
GR  - N01-HB-4-7003/HB/NHLBI NIH HHS/United States
PT  - Clinical Trial
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Clin Immunol Immunopathol
JT  - Clinical immunology and immunopathology
JID - 0356637
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/*blood/immunology/transmission
MH  - Blood Coagulation Disorders/*blood/immunology
MH  - Child
MH  - *Communicable Disease Control
MH  - Flow Cytometry
MH  - *HIV-1
MH  - Humans
MH  - Longitudinal Studies
MH  - Lymphocytes/*classification
MH  - Male
MH  - Multicenter Studies as Topic
MH  - Phenotype
MH  - *Transfusion Reaction
EDAT- 1989/07/01 00:00
MHDA- 1989/07/01 00:01
CRDT- 1989/07/01 00:00
PHST- 1989/07/01 00:00 [pubmed]
PHST- 1989/07/01 00:01 [medline]
PHST- 1989/07/01 00:00 [entrez]
AID - 10.1016/0090-1229(89)90191-8 [doi]
PST - ppublish
SO  - Clin Immunol Immunopathol. 1989 Jul;52(1):38-47. doi: 
      10.1016/0090-1229(89)90191-8.